Cited by 361DNA Repair MechanismsGenomics and Chromatin DynamicsRNA Research and Splicing

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Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors

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Pessina, Fabio, Giavazzi, Fabio, Yin, Yandong, Gioia, Ubaldo, Vitelli, Valerio, Galbiati, Alessandro, Barozzi, Sara, Garre, Massimiliano, Oldani, Amanda, Flaus, Andrew, Cerbino, Roberto, Parazzoli, Dario, Rothenberg, Eli, d’Adda di Fagagna, Fabrizio·2019·Nature Cell Biology

DOI 10.1038/s41556-019-0392-4PMC PMC6859070

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Abstract

Damage-induced long non-coding RNAs (dilncRNA) synthesized at DNA double-strand breaks (DSBs) by RNA polymerase II are necessary for DNA-damage-response (DDR) focus formation. We demonstrate that induction of DSBs results in the assembly of functional promoters that include a complete RNA polymerase II preinitiation complex, MED1 and CDK9. Absence or inactivation of these factors causes a reduction in DDR foci both in vivo and in an in vitro system that reconstitutes DDR events on nucleosomes. We also show that dilncRNAs drive molecular crowding of DDR proteins, such as 53BP1, into foci that exhibit liquid-liquid phase-separation condensate properties. We propose that the assembly of DSB-induced transcriptional promoters drives RNA synthesis, which stimulates phase separation of DDR factors in the shape of foci.

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Authors: Pessina, F., Giavazzi, F., Yin, Y., Gioia, U., Vitelli, V., Galbiati, A., Barozzi, S., Garre, M., Oldani, A., Flaus, A., Cerbino, R., Parazzoli, D., Rothenberg, E., & d’Adda di Fagagna, F.
Affiliations: IFOM (IT)
University of Milan (IT)
New York University (US)
Ollscoil na Gaillimhe – University of Galway (IE)
ORCID: Giavazzi, Fabio
Yin, Yandong
Gioia, Ubaldo
Galbiati, Alessandro
Barozzi, Sara
Flaus, Andrew
Cerbino, Roberto
Parazzoli, Dario
Rothenberg, Eli
d’Adda di Fagagna, Fabrizio
Venue: Nature Cell Biology
Publisher: Springer Science and Business Media LLC
Published: 2019-09-30
Volume: 21
Issue: 10
Pages: 1286-1299
ISSN: 1465-7392 (ISSN-L)
1476-4679
DOI: 10.1038/s41556-019-0392-4
PMC: PMC6859070
OpenAlex: W2975196800
Cited by: 360
References: 86
Citations by year: 2026: 31 · 2025: 48 · 2024: 53 · 2023: 49 · 2022: 63 · 2021: 71 · 2020: 42 · 2019: 2
FWCI: 14.72
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Nature Cell Biology (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) (submittedVersion, other-oa)
Funders: Marie Curie international mobility fellowship part of Structured International Post Doc Program
Fondazione Cariplo (2016-0998)
Regione Lombardia
Associazione Italiana per la Ricerca sul Cancro (MFAG 2018-22083)
AriSLA
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords: DNA double-strand breaks, MED1, CDK9, 53BP1, liquid-liquid phase separation, condensate, macromolecular crowding, nucleosome reconstitution, transcriptional promoter
MeSH terms: Tumor Suppressor p53-Binding Protein 1, DNA, DNA Repair, Gene Expression Regulation, HeLa Cells, Histones, Humans, Osteoblasts, Promoter Regions, Genetic, RNA Polymerase II, Transcription, Genetic, Signal Transduction, Cyclin-Dependent Kinase 9, Cell Line, Tumor, DNA Breaks, Double-Stranded, Mediator Complex Subunit 1, HEK293 Cells, RNA, Long Noncoding, Ataxia Telangiectasia Mutated Proteins

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Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors

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