The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker
Open accessAbstract
Exosomes are extracellular vesicles derived from the endosomal compartment that are potentially involved in intercellular communication. Here, we found that frequently used biomarkers of exosomes are heterogeneous, and do not exhibit universal utility across different cell types. To uncover ubiquitous and abundant proteins, we used an unbiased and quantitative proteomic approach based on super-stable isotope labeling with amino acids in cell culture (super-SILAC), coupled to high-resolution mass spectrometry. In total, 1,212 proteins were quantified in the proteome of exosomes, irrespective of the cellular source or isolation method. A cohort of 22 proteins was universally enriched. Fifteen proteins were consistently depleted in the proteome of exosomes compared to cells. Among the enriched proteins, we identified biogenesis-related proteins, GTPases and membrane proteins, such as CD47 and ITGB1. The cohort of depleted proteins in exosomes was predominantly composed of nuclear proteins. We identified syntenin-1 as a consistently abundant protein in exosomes from different cellular origins. Syntenin-1 is also present in exosomes across different species and biofluids, highlighting its potential use as a putative universal biomarker of exosomes. Our study provides a comprehensive quantitative atlas of core proteins ubiquitous to exosomes that can serve as a resource for the scientific community. Using an unbiased and quantitative proteomic approach, Kugeratski et al. identified the core constituents of exosomes and found that syntenin-1 was the most abundant component, making it a putative universal biomarker.
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Extracellular Vesicles and Nanoparticles: Emerging Complexities
Jeppesen, D. K., Zhang, Q., Franklin, J. L., & Coffey, R. J. (2023) · Trends in Cell Biology · doi:10.1016/j.tcb.2023.01.002 · Cited by 705
The role of extracellular vesicles in cancer
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Self-citationCurrent advance of nanotechnology in diagnosis and treatment for malignant tumors
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Regulation of cargo selection in exosome biogenesis and its biomedical applications in cancer
Lee, Y. J., Shin, K. J., & Chae, Y. C. (2024) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01209-y · Cited by 427
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Metadata
- Authors
- Kugeratski, F. G., Hodge, K., Lilla, S., McAndrews, K. M., Zhou, X., Hwang, R. F., Zanivan, S., & Kalluri, R.
- Venue
- Nature Cell Biology
- Published
- 2021-06
- Volume
- 23
- Issue
- 6
- Pages
- 631-641
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W3168265815
- Cited by
- 488
- References
- 58
- FWCI
- 20.80
- Type
- journal article
- Language
- en
- Open access
- green
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- Kugeratski, F. G., Hodge, K., Lilla, S., McAndrews, K. M., Zhou, X., Hwang, R. F., Zanivan, S., & Kalluri, R. (2021). Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker. Nature Cell Biology. https://doi.org/10.1038/s41556-021-00693-y
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