Cited by 65Electrochemical sensors and biosensorsNeutrophil, Myeloperoxidase and Oxidative MechanismsOccupational and environmental lung diseases

Nanosensor detection of reactive oxygen and nitrogen species leakage in frustrated phagocytosis of nanofibres

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Qi, Yu-Ting, Zhang, Fu-Li, Tian, Si-Yu, Wu, Hui-Qian, Zhao, Yi, Zhang, Xin-Wei, Liu, Yan-Ling, Fu, Pingqing +2 more·2024·Nature Nanotechnology

Abstract

Exposure to widely used inert fibrous nanomaterials (for example, glass fibres or carbon nanotubes) may result in asbestos-like lung pathologies, becoming an important environmental and health concern. However, the origin of the pathogenesis of such fibres has not yet been clearly established. Here we report an electrochemical nanosensor that is used to monitor and quantitatively characterize the flux and dynamics of reactive species release during the frustrated phagocytosis of glass nanofibres by single macrophages. We show the existence of an intense prolonged release of reactive oxygen and nitrogen species by single macrophages near their phagocytic cups. This continued massive leakage of reactive oxygen and nitrogen species damages peripheral cells and eventually translates into chronic inflammation and lung injury, as seen during in vitro co-culture and in vivo experiments.

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  • Macrophages tend to stall uptake of fibres longer than their own size if the cell membrane cannot sufficiently be deformed to enclose the whole fibre, leaving the cell penetrated (frustrated phagocytosis).

    “Macrophages tend to stall the uptake of fibres longer than their own size if the cell membrane cannot sufficiently be deformed to enclose the whole fibre. This so-called ‘frustrated phagocytosis’ leaves the cell in a penetrated state [11, 12].”

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Authors
Qi, Y.-T., Zhang, F.-L., Tian, S.-Y., Wu, H.-Q., Zhao, Y., Zhang, X.-W., Liu, Y.-L., Fu, P., Amatore, C., & Huang, W.-H.
Affiliations
  • Wuhan University (CN)
  • Tianjin University (CN)
  • Xiamen University (CN)
Corresponding author
  • Amatore, Christian
  • Huang, Wei-Hua
Venue
Nature Nanotechnology
Published
2024-01-03
Volume
19
Issue
4
Pages
524-533
ISSN
  • 1748-3387 (ISSN-L)
  • 1748-3395
OpenAlex
W4390570325
Cited by
65
References
50
Citations by year
2026: 24 · 2025: 28 · 2024: 13
FWCI
6.73
Type
journal article
Language
en
Open access
Locations
  • Nature Nanotechnology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
Funders
National Natural Science Foundation of China (22090050, 22090051, 21721005, 21725504, 22122408, 42221001)
Topics
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Occupational and environmental lung diseases
Keywords
reactive oxygen species, reactive nitrogen species, frustrated phagocytosis, electrochemical nanosensor, macrophages, chronic inflammation, phagocytic cup
MeSH terms
Macrophages, Oxygen, Phagocytosis, Reactive Oxygen Species, Nanotubes, Carbon, Nanofibers

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