Nanosensor detection of reactive oxygen and nitrogen species leakage in frustrated phagocytosis of nanofibres
Open accessAbstract
Exposure to widely used inert fibrous nanomaterials (for example, glass fibres or carbon nanotubes) may result in asbestos-like lung pathologies, becoming an important environmental and health concern. However, the origin of the pathogenesis of such fibres has not yet been clearly established. Here we report an electrochemical nanosensor that is used to monitor and quantitatively characterize the flux and dynamics of reactive species release during the frustrated phagocytosis of glass nanofibres by single macrophages. We show the existence of an intense prolonged release of reactive oxygen and nitrogen species by single macrophages near their phagocytic cups. This continued massive leakage of reactive oxygen and nitrogen species damages peripheral cells and eventually translates into chronic inflammation and lung injury, as seen during in vitro co-culture and in vivo experiments.
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Macrophages tend to stall uptake of fibres longer than their own size if the cell membrane cannot sufficiently be deformed to enclose the whole fibre, leaving the cell penetrated (frustrated phagocytosis).
“Macrophages tend to stall the uptake of fibres longer than their own size if the cell membrane cannot sufficiently be deformed to enclose the whole fibre. This so-called ‘frustrated phagocytosis’ leaves the cell in a penetrated state [11, 12].”
From Macrophages bend long fibres with flexural rigidity lower than 3 mN·nm2 to avoid frustrated phagocytosis (2026) · cites this paper as [12]
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- Authors
- Qi, Y.-T., Zhang, F.-L., Tian, S.-Y., Wu, H.-Q., Zhao, Y., Zhang, X.-W., Liu, Y.-L., Fu, P., Amatore, C., & Huang, W.-H.
- Affiliations
- Wuhan University (CN)
- Tianjin University (CN)
- Xiamen University (CN)
- Corresponding author
- Amatore, Christian
- Huang, Wei-Hua
- ORCID
- Venue
- Nature Nanotechnology
- Published
- 2024-01-03
- Volume
- 19
- Issue
- 4
- Pages
- 524-533
- ISSN
- 1748-3387 (ISSN-L)
- 1748-3395
- OpenAlex
- W4390570325
- Cited by
- 65
- References
- 50
- Citations by year
- 2026: 24 · 2025: 28 · 2024: 13
- FWCI
- 6.73
- Type
- journal article
- Language
- en
- Open access
- green
- hal.science
- Licence
- Locations
- Nature Nanotechnology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Funders
- National Natural Science Foundation of China (22090050, 22090051, 21721005, 21725504, 22122408, 42221001)
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Occupational and environmental lung diseases
- Keywords
- reactive oxygen species, reactive nitrogen species, frustrated phagocytosis, electrochemical nanosensor, macrophages, chronic inflammation, phagocytic cup
- MeSH terms
- Macrophages, Oxygen, Phagocytosis, Reactive Oxygen Species, Nanotubes, Carbon, Nanofibers
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