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SLC25A51 is a mammalian mitochondrial NAD(+) transporter
Open accessAbstract
Mitochondria require nicotinamide adenine dinucleotide (NAD+) to carry out the fundamental processes that fuel respiration and mediate cellular energy transduction. Mitochondrial NAD+ transporters have been identified in yeast and plants 1,2, but their existence in mammals remains controversial 3-5. Here we demonstrate that mammalian mitochondria can take up intact NAD+, and identify SLC25A51 (also known as MCART1)-an essential 6,7 mitochondrial protein of previously unknown function-as a mammalian mitochondrial NAD+ transporter. Loss of SLC25A51 decreases mitochondrial-but not whole-cell-NAD+ content, impairs mitochondrial respiration, and blocks the uptake of NAD+ into isolated mitochondria. Conversely, overexpression of SLC25A51 or SLC25A52 (a nearly identical paralogue of SLC25A51) increases mitochondrial NAD+ levels and restores NAD+ uptake into yeast mitochondria lacking endogenous NAD+ transporters. Together, these findings identify SLC25A51 as a mammalian transporter capable of importing NAD+ into mitochondria.
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“Notably, while NAD+ itself can be imported into mitochondria through SLC25A51–MCART1 (98, 99, 100), NADH is not efficiently transported.”
From Regulation and function of the mammalian tricarboxylic acid cycle (2023) · cites this paper as [100]
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Metadata
- Authors
- Luongo, T. S., Eller, J. M., Lu, M.-J., Niere, M., Raith, F., Perry, C., Bornstein, M. R., Oliphint, P., Wang, L., McReynolds, M. R., Migaud, M. E., Rabinowitz, J. D., Johnson, F. B., Johnsson, K., Ziegler, M., Cambronne, X. A., & Baur, J. A.
- Affiliations
- University of Pennsylvania (US)
- The University of Texas at Austin (US)
- University of Bergen (NO)
- Heidelberg University (DE)
- Princeton University (US)
- USA Mitchell Cancer Institute (US)
- Max Planck Institute for Medical Research (DE)
- Corresponding author
- Cambronne, Xiaolu A.
- Baur, Joseph A.
- ORCID
- Venue
- Nature
- Published
- 2020-09-09
- Volume
- 588
- Issue
- 7836
- Pages
- 174-179
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC7718333
- OpenAlex
- W3084174900
- Cited by
- 308
- References
- 44
- Citations by year
- 2026: 39 · 2025: 60 · 2024: 56 · 2023: 56 · 2022: 48 · 2021: 46 · 2020: 3
- FWCI
- 12.41
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- NORA - Norwegian Open Research Archives (submittedVersion, other-oa)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- National Institutes of Health (TL1TR001880)
- National Heart, Lung, and Blood Institute (F32HL145923)
- National Institute of General Medical Sciences (DP2GM126897)
- National Institute of Diabetes and Digestive and Kidney Diseases (P30DK019525, R01DK098656)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (T32AR053461)
- National Center for Advancing Translational Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Keywords
- mitochondrial respiration, yeast mitochondria
- MeSH terms
- Animals, Biological Transport, Cell Line, Genetic Complementation Test, Humans, Mitochondria, NAD, Saccharomyces cerevisiae, Cell Respiration, Mitochondrial Proteins, Organic Cation Transport Proteins, Saccharomyces cerevisiae Proteins, Nucleotide Transport Proteins, Mice
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