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Cited by 840Adipose Tissue and MetabolismSingle-cell and spatial transcriptomicsAdipokines, Inflammation, and Metabolic Diseases

A single-cell atlas of human and mouse white adipose tissue

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Emont, Margo P., Jacobs, Christopher, Essene, Adam L., Pant, Deepti, Tenen, Danielle, Colleluori, Georgia, Di Vincenzo, Angelica, Jørgensen, Anja M. +30 more·2022·Nature
DOI 10.1038/s41586-022-04518-2PMC PMC9504827

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Abstract

White adipose tissue, once regarded as morphologically and functionally bland, is now recognized to be dynamic, plastic and heterogenous, and is involved in a wide array of biological processes including energy homeostasis, glucose and lipid handling, blood pressure control and host defence 1. High-fat feeding and other metabolic stressors cause marked changes in adipose morphology, physiology and cellular composition 1, and alterations in adiposity are associated with insulin resistance, dyslipidemia and type 2 diabetes 2. Here we provide detailed cellular atlases of human and mouse subcutaneous and visceral white fat at single-cell resolution across a range of body weight. We identify subpopulations of adipocytes, adipose stem and progenitor cells, vascular and immune cells and demonstrate commonalities and differences across species and dietary conditions. We link specific cell types to increased risk of metabolic disease and provide an initial blueprint for a comprehensive set of interactions between individual cell types in the adipose niche in leanness and obesity. These data comprise an extensive resource for the exploration of genes, traits and cell types in the function of white adipose tissue across species, depots and nutritional conditions.

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Cited by

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Authors
Emont, M. P., Jacobs, C., Essene, A. L., Pant, D., Tenen, D., Colleluori, G., Di Vincenzo, A., Jørgensen, A. M., Dashti, H., Stefek, A., McGonagle, E., Strobel, S., Laber, S., Agrawal, S., Westcott, G. P., Kar, A., Veregge, M. L., Gulko, A., Srinivasan, H., Kramer, Z., De Filippis, E., Merkel, E., Ducie, J., Boyd, C. G., Gourash, W., Courcoulas, A., Lin, S. J., Lee, B. T., Morris, D., Tobias, A., Khera, A. V., Claussnitzer, M., Pers, T. H., Giordano, A., Ashenberg, O., Regev, A., Tsai, L. T., & Rosen, E. D.
Affiliations
  • Broad Institute (US)
  • Beth Israel Deaconess Medical Center (US)
  • Marche Polytechnic University (IT)
  • University of Copenhagen (DK)
  • University of Pittsburgh Medical Center (US)
Corresponding author
Rosen, Evan D.
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2022-03-16
Volume
603
Issue
7903
Pages
926-933
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/s41586-022-04518-2
PMC
PMC9504827
OpenAlex
W4221010612
Cited by
840
References
16
Citations by year
2026: 193 · 2025: 218 · 2024: 202 · 2023: 161 · 2022: 65
FWCI
100.17
Type
journal article
Language
en
Update notices
correction (2023-07-26)
Open access
Licence
Locations
  • Nature (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • Università Politecnica delle Marche (Università Politecnica delle Marche) (submittedVersion, other-oa)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Funders
  • Foundation for the National Institutes of Health (RC2 DK116691)
  • American Heart Association
  • Boston Area Diabetes Endocrinology Research Center (5P30DK057521)
  • Broad Institute
  • Sarnoff Cardiovascular Research Foundation
  • Lundbeckfonden (R190 2014-3904)
  • Novo Nordisk (NNF18CC0034900)
  • Novo Nordisk Fonden (NNF 18 CC0034900)
  • Novo Nordisk Foundation Center for Basic Metabolic Research (NNF 18 CC0034900)
  • H. Lundbeck A/S
  • National Institutes of Health
  • Boston Nutrition Obesity Research Center, Boston University
  • National Human Genome Research Institute (K08HG010155, U01HG011719)
  • National Institute of Diabetes and Digestive and Kidney Diseases (P30DK057521, UM1DK126185, P30DK046200, F32DK124914, R01DK087092, DK116691, R01DK102173, T32DK007516, RC2DK116691)
Topics
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
white adipose tissue, single-cell atlas, adipocytes, subcutaneous adipose tissue, visceral fat, obesity, leanness, high-fat diet, metabolic disorders, type 2 diabetes, insulin resistance, human-mouse comparison
MeSH terms
Adipose Tissue, Animals, Atlases as Topic, Diabetes Mellitus, Type 2, Humans, Insulin Resistance, Metabolic Diseases, Obesity, Adiposity, Mice, Adipose Tissue, White

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