The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Plant gene editing through de novo induction of meristems
Open accessAbstract
Plant gene editing is typically performed by delivering reagents such as Cas9 and single guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole plants by exposure to various hormones. The creation of edited plants through tissue culture is often inefficient, time-consuming, works for only limited species and genotypes, and causes unintended changes to the genome and epigenome. Here we report two methods to generate gene-edited dicotyledonous plants through de novo meristem induction. Developmental regulators and gene-editing reagents are delivered to somatic cells of whole plants. This induces meristems that produce shoots with targeted DNA modifications, and gene edits are transmitted to the next generation. The de novo induction of gene-edited meristems sidesteps the need for tissue culture and promises to overcome a bottleneck in plant gene editing.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Injection of Agrobacterium tumefaciens carrying the development regulators WUS2 and SHOOT MERISTEMLESS (STM) into tobacco seedlings led to the development of gene-edited shoots with heritable mutations (Maher et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Maher et al., 2020
In the citing paper
“De novo induction of geneedited meristems also avoids the tissue culture step and can be induced in aseptically grown seedlings and soil-grown plants (Maher et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Maher et al., 2020
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana
Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 22,014
Isolation of plant DNA from fresh tissue
Doyle, J. J. (1990) · Focus eBooks · Cited by 10,802
Easy quantitative assessment of genome editing by sequence trace decomposition
Brinkman, E. K., Chen, T., Amendola, M., & van Steensel, B. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku936 · Cited by 2,390
Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
Gelvin, S. B. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.1.16-37.2003 · Cited by 1,383
Comparison of Sequencing Utility Programs
Aronesty, E. (2013) · The Open Bioinformatics Journal · doi:10.2174/1875036201307010001 · Cited by 1,144
Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation
Lowe, K., Wu, E., Wang, N., Hoerster, G., Hastings, C., Cho, M.-J., Scelonge, C., Lenderts, B., Chamberlin, M., Cushatt, J., et al. (2016) · The Plant Cell · doi:10.1105/tpc.16.00124 · Cited by 881
Advancing Crop Transformation in the Era of Genome Editing
Altpeter, F., Springer, N. M., Bartley, L., Blechl, A. E., Brutnell, T. P., Citovsky, V., Conrad, L. J., Gelvin, S. B., Jackson, D. P., Kausch, A. P., et al. (2016) · The Plant Cell · doi:10.1105/tpc.16.00196 · Cited by 707
Self-citationA Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants
Čermák, T., Curtin, S. J., Gil‐Humanes, J., Čegan, R., Kono, T. J. Y., Konečná, E., Belanto, J. J., Starker, C. G., Mathre, J. W., Greenstein, R. L., et al. (2017) · The Plant Cell · doi:10.1105/tpc.16.00922 · Cited by 668
Self-citationGenetic instability of plant tissue cultures: breakdown of normal controls.
Phillips, R. L., Kaeppler, S. M., & Olhoft, P. M. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.12.5222 · Cited by 582
DNA Replicons for Plant Genome Engineering
Baltes, N. J., Gil‐Humanes, J., Čermák, T., Atkins, P., & Voytas, D. F. (2014) · The Plant Cell · doi:10.1105/tpc.113.119792 · Cited by 552
Self-citation
Cited by
Sorted by citations
Items 201–300 of 539
Kshetry, A. O., Ghose, K., Alok, A., Devkar, V., Raman, V., Stupar, R. M., Herrera‐Estrella, L., Zhang, F., & Patil, G. (2025) · Molecular Plant · doi:10.1016/j.molp.2025.09.017 · Cited by 33
CRISPR Genome Editing Technology: A Powerful Tool Applied to Developing Agribusiness
Maximiano, M. R., Távora, F. T. P. K., Prado, G. S., Dias, S. C., Mehta, Â., & Franco, O. L. (2021) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.1c01062 · Cited by 25
Duan, H., Maren, N. A., Ranney, T. G., & Liu, W. (2022) · Ornamental Plant Research · doi:10.48130/opr-2022-0004 · Cited by 23
Liu, S., Li, L., Deng, Y., Bai, Y., Sun, C., Huang, S., Zhou, J., Liyu, S., Yang, X., Li, L., et al. (2022) · Horticulture Research · doi:10.1093/hr/uhac044 · Cited by 23
Subburaj, S., Zanatta, C. B., Nunn, J. A. L., Hoepers, A. M., Nodari, R. O., & Agapito-Tenfen, S. Z. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.939997 · Cited by 25
CRISPR/Cas mediated genome editing in potato: Past achievements and future directions
Tuncel, A., & Qi, Y. (2022) · Plant Science · doi:10.1016/j.plantsci.2022.111474 · Cited by 25
Recent Progress in Genetic Transformation and Gene Editing Technology in Cucurbit Crops
Feng, J., Wang, N., Yang, L., Wang, H. H., Zhang, W., Wang, H., & Chai, S. (2023) · Agronomy · doi:10.3390/agronomy13030755 · Cited by 26
Potential applications of the CRISPR/Cas technology for genetic improvement of yam ( Dioscorea spp.)
Syombua, E. D., Tripathi, J. N., Obiero, G. O., Nguu, E. K., Yang, B., Wang, K., & Tripathi, L. (2021) · Food and Energy Security · doi:10.1002/fes3.330 · Cited by 24
Kim, S. Y., Wengier, D. L., Ragland, C. J., & Sattely, E. S. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00289 · Cited by 22
Liu, W., Zheng, T., Yang, Y., Li, P., Qiu, L., Li, L., Wang, J., Cheng, T., & Zhang, Q. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.781343 · Cited by 21
Wan, L., Wang, Z., Zhang, X., Zeng, H., Ren, J. M., Zhang, N., Sun, Y., & Tang, M. (2023) · Genes · doi:10.3390/genes14071432 · Cited by 21
Karthik, K., Mishra, P., Bajpai, A., Sharma, M., Rao, U., & Sreevathsa, R. (2020) · Physiology and Molecular Biology of Plants · doi:10.1007/s12298-020-00887-y · Cited by 21
Crandall, S. G., Spychalla, J., Crouch, U. T., Acevedo, F. E., Naegele, R. P., & Miles, T. D. (2022) · Plant Disease · doi:10.1094/pdis-04-21-0695-fe · Cited by 20
“CRISPR gene editing is being developed for grape and could be a promising alternative or supplement to traditional breeding methods (Maher et al. 2019) (Figs.”
Nanotechnology and CRISPR/Cas9 system for sustainable agriculture
Khanna, K., Ohri, P., & Bhardwaj, R. (2023) · Environmental Science and Pollution Research · doi:10.1007/s11356-023-26482-8 · Cited by 22
Suspension culture of stem cells established of Calendula officinalis L.
Kaňuková, Š., Lenkavská, K., Gubišová, M., & Kraic, J. (2024) · Scientific Reports · doi:10.1038/s41598-023-50945-0 · Cited by 20
“Shoots and roots can be regenerated through de novo-formed meristems 52 and by gene editing after the concomitant expression of developmental regulators and gene-editing reagents 53 .”
BackgroundVersatile in vitro assay to recognize Cas9‐induced mutations
Bente, H., Scheid, O. M., & Donà, M. (2020) · Plant Direct · doi:10.1002/pld3.269 · Cited by 20
Morphogenic Regulators and Their Application in Improving Plant Transformation
Nalapalli, S., Tunc‐Ozdemir, M., Sun, Y., Elumalai Sivamani, A., & Que, Q. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1068-8_3 · Cited by 21
Karunarathne, S., Walker, E., Sharma, D., Li, C., & Han, Y. (2023) · Journal of Zhejiang University-SCIENCE B · doi:10.1631/jzus.b2200552 · Cited by 18
“Injection of Agrobacterium tumefaciens carrying the develop‐ ment regulators WUS2 and SHOOT MERISTEMLESS ( STM ) into tobacco seedlings led to the development of gene-edited shoots with heritable mutations (Maher et al., 2020).”
Moonsub, K., Seesuriyachan, P., Boonyawan, D., & Wattanutchariya, W. (2024) · Processes · doi:10.3390/pr12030567 · Cited by 19
Crop genome editing through tissue-culture-independent transformation methods
Sebiani-Calvo, A., Hernández-Soto, A., Hensel, G., & Gatica-Arias, A. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2024.1490295 · Cited by 19
Lokya, V., Singh, S., Chaudhary, R., Jangra, A., & Tiwari, S. (2025) · Plant Molecular Biology · doi:10.1007/s11103-025-01600-x · Cited by 21
“Several studies have deployed smaller Cas9 variants, such as Cas12a/Cpf1 and TnpB, through viral vectors and Agrobacterium to overcome the genetic cargo constraints and off-target effects (Acharya et al. 2019; Maher et al. 2020; Karmakar et al. 2024).”
Progress and challenges in applying CRISPR/Cas techniques to the genome editing of trees
Pak, S., & Li, C. (2022) · Forestry Research · doi:10.48130/fr-2022-0006 · Cited by 20
CRISPR-edited plants by grafting
Hu, J., & Gao, C. (2023) · Nature Biotechnology · doi:10.1038/s41587-022-01516-7 · Cited by 19
CRISPR gene editing to improve crop resistance to parasitic plants
Jhu, M.-Y., Ellison, E. E., & Sinha, N. R. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1289416 · Cited by 20
CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products
Nuccio, M. L., Claeys, H., & Heyndrickx, K. S. (2021) · Molecular Breeding · doi:10.1007/s11032-021-01200-9 · Cited by 21
Maize tissue culture, transformation, and genome editing
Kausch, A. P., Nelson‐Vasilchik, K., Tilelli, M., & Hague, J. P. (2021) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-021-10196-y · Cited by 19
Critical considerations and computational tools in plant genome editing
Saha, D., Panda, A. K., & Datta, S. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e41135 · Cited by 18
Genome Editing in Crop Plant Research—Alignment of Expectations and Current Developments
Hüdig, M., Laibach, N., & Hein, A.-C. (2022) · Plants · doi:10.3390/plants11020212 · Cited by 18
“Methods, such as in vitro assembled ribonucleoprotein particles (RNPs), virus-mediated delivery of RNA templates, nanoparticles, or agrobacterium-mediated vesicle delivery, have been established for several plant species [49,50]; adapted guide RNA usage [40] and approaches, such as de novo meristemisation, aim to avoi…”
BackgroundBiological and Molecular Components for Genetically Engineering Biosensors in Plants
Liu, Y., Yuan, G., Hassan, M. M., Abraham, P. E., Mitchell, J. C., Jacobson, D., Tuskan, G. A., Khakhar, A., Medford, J. I., Zhao, C., et al. (2022) · BioDesign Research · doi:10.34133/2022/9863496 · Cited by 18
“In addition, developmental regulators have been used together with gene editing reagents to generate gene-edited plants through de novo meristem induction, thereby, bypassing the tissue culture process [106].”
MethodShelake, R. M., Jadhav, A. M., Bhosale, P. B., & Kim, J. (2023) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2023.108070 · Cited by 18
Genome editing based trait improvement in crops: current perspective, challenges and opportunities
Singh, S., Chaudhary, R., Lokya, V., & Tiwari, S. (2024) · The Nucleus · doi:10.1007/s13237-024-00472-8 · Cited by 18
The evolving landscape of precise DNA insertion in plants
Vu, T. V., Nguyen, N. T., Kim, J., Sung, Y. W., Chung, W. S., & Kim, J. (2025) · Nature Communications · doi:10.1038/s41467-025-66715-7 · Cited by 21
“The process of inserting DNA into plants is often constrained by the dependence on these methods, which typically limit applicability to a narrow range of genotypes that are capable of forming callus and regenerating somatic shoots 143,144 .”
CRISPR/Cas9: an advanced platform for root and tuber crops improvement
Divya, K., Thangaraj, M., & Radhika, N. K. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1242510 · Cited by 20
MIR396-GRF/GIF enhances in planta shoot regeneration of Dendrobium catenatum
Yang, Z., Zhao, M., Zhang, X., Gu, L., Jian, L., Ming, F., Wang, M., & Wang, Z. (2024) · BMC Genomics · doi:10.1186/s12864-024-10360-9 · Cited by 17
“Another strategy was to use different combinations of DG s to induce de novo meristems in dicots without tissue culture [30].”
MethodRoychowdhury, R., Das, S. P., Das, S., Biswas, S., Patel, M. K., Kumar, A., Sarker, U., Pal, S., Das, R., Yogendra, K. N., et al. (2025) · Functional & Integrative Genomics · doi:10.1007/s10142-025-01533-0 · Cited by 20
Advances in CRISPR/Cas9-mediated genome editing on vegetable crops
Tian, S., Xing, S., & Xu, Y. (2021) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-021-10187-z · Cited by 17
“Another strategy is to bypass the tissue culture stage and directly produce genome-edited plants by co-expressing developmental regulatory genes (Maher et al. 2020).”
BackgroundUnlocking the potential of genome editing in agriculture with tissue culture‐free techniques
Hu, M., & Liu, D. (2025) · New Phytologist · doi:10.1111/nph.70046 · Cited by 16
Advances and prospects of large DNA fragment editing in plants
Zhao, Y., Liang, Y., Ni, Z., Sun, Q., Zong, Y., & Wang, Y. (2025) · Nature Plants · doi:10.1038/s41477-025-02160-0 · Cited by 17
“Finally, novel approaches, such as de novo meristem induction and viral delivery systems, could completely bypass the regeneration bottleneck 116–118 .”
Reprogramming plant specialized metabolism by manipulating protein kinases
Lyu, R., Singh, S. K., Liu, Y., Patra, B., Zhou, Y., Wang, B., Pattanaik, S., & Yuan, L. (2021) · aBIOTECH · doi:10.1007/s42994-021-00053-2 · Cited by 16
“…plant paper), hairy roots, cell cultures, or modified Agrobacterium-mediated gene delivery and Agricultural Information Institute, Chinese Academy of Agricultural Sciences 2021 234 aBIOTECH (2021) 2:226–239 virus-based gene silencing are effective alternatives (Ma et al. 2020; Maher et al. 2020).”
BackgroundImproving crops through transgenic breeding—Technological advances and prospects
Gantait, S., Mukherjee, E., Jogam, P., Babu, K. H., Jain, S. M., & Suprasanna, P. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-90795-8.00011-4 · Cited by 17
Updates and Applications of CRISPR/Cas Technology in Plants
Vidya, N., & Arun, M. (2023) · Journal of Plant Biology · doi:10.1007/s12374-023-09383-8 · Cited by 18
Yarra, R., & Krysan, P. J. (2023) · Planta · doi:10.1007/s00425-023-04096-1 · Cited by 16
Hao, S., Zhang, Y., Li, R., Qu, P., & Cheng, C. (2023) · Scientia Horticulturae · doi:10.1016/j.scienta.2023.112693 · Cited by 16
Rehman, R. S., Zafar, S. A., Ali, M., Pasha, A. N., Naveed, M. S., Waseem, M., Ahmad, M., & Raza, A. (2022) · Asian Plant Research Journal · doi:10.9734/aprj/2022/v9i130197 · Cited by 16
Development of high‐throughput tissue culture‐free plant transformation systems
Zhong, H., Elumalai Sivamani, A., Li, C., Liu, W., Dong, S., & Que, Q. (2024) · The Plant Journal · doi:10.1111/tpj.17163 · Cited by 15
Emerging frontiers in sorghum genetic engineering
Nigam, D., Devkar, V., Dhiman, P., Shakoor, S., Liu, D., Patil, G., & Jiao, Y. (2025) · The Plant Journal · doi:10.1111/tpj.17244 · Cited by 15
Nanomaterial-based gene delivery in plants: an upcoming genetic revolution?
Kandhol, N., Dash, P. K., Singh, V. P., Deshmukh, R. K., Dhankher, O. P., Tran, L. P., White, J. C., & Tripathi, D. K. (2025) · Trends in Plant Science · doi:10.1016/j.tplants.2025.04.012 · Cited by 15
Woody Plant Transformation: Current Status, Challenges, and Future Perspectives
Maharjan, B., Islam, M. T., Muzaffar, A., Tschaplinski, T. J., Tuskan, G. A., Chen, J., & Yang, X. (2025) · Plants · doi:10.3390/plants14223420 · Cited by 21
“The Fast-TrACC (fast-treated Agrobacterium co-culture at the seedling stage) approach has produced bioluminescent shoots in grapes [77].”
Fast-TrACC: A Rapid Method for Delivering and Testing Gene Editing Reagents in Somatic Plant Cells
Nasti, R. A., Zinselmeier, M. H., Vollbrecht, M., Maher, M. F., & Voytas, D. F. (2021) · Frontiers in Genome Editing · doi:10.3389/fgeed.2020.621710 · Cited by 15
Self-citationLiu, L., Lindsay, P. L., & Jackson, D. P. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22105167 · Cited by 15
A case study of using an efficient CRISPR/Cas9 system to develop variegated lettuce
Nguyen, C. D., Li, J., Mou, B., Gong, H., & Huo, H. (2021) · Vegetable Research · doi:10.48130/vr-2021-0004 · Cited by 15
Nuzzo, F., Gambino, G., & Perrone, I. (2022) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2022.10.027 · Cited by 15
Viral vectors as carriers of genome-editing reagents
Awan, M. J. A., Akram, A., Amin, I. U., & Mansoor, S. (2023) · Trends in Plant Science · doi:10.1016/j.tplants.2023.05.010 · Cited by 15
Wang, X., Gu, X., Xu, Z., Yin, Z., Yang, X., Lin, R., Quannan, Z., Huang, H., & Huang, T. (2023) · Forests · doi:10.3390/f14091891 · Cited by 18
Tiedge, K., Muchlinski, A., & Zerbe, P. (2020) · Synthetic Biology · doi:10.1093/synbio/ysaa005 · Cited by 15
“limitations in regenerating transgenic plants from tissue culture (27, 94).”
BackgroundCao, X., Qin, Z., Fan, P., Wang, S., Meng, X., Wan, H., Yang, W., Chen, S., Yao, H., Chen, W., et al. (2025) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2025.03.038 · Cited by 14
Efficient base editing in tomato using a highly expressed transient system
Yuan, S., Kawasaki, S., Abdellatif, I. M. Y., Nishida, K., Kondo, A., Ariizumi, T., Ezura, H., & Miura, K. (2021) · Plant Cell Reports · doi:10.1007/s00299-021-02662-z · Cited by 14
Identifying Molecular Chechkpoints for Adventitious Root Induction: Are We Ready to Fill the Gaps?
Abarca, D. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.621032 · Cited by 14
CRISPR/Cas in Grapevine Genome Editing: The Best Is Yet to Come
Ren, C., Mohamed, M. S. M., Aini, N., Kuang, Y., & Liang, Z. (2024) · Horticulturae · doi:10.3390/horticulturae10090965 · Cited by 18
“A proof-of-concept study had been conducted in grapevine, and transgenic plants with luciferase luminescence were successfully produced [175].”
Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing
Han, X., Deng, Z., Liu, H., & Ji, X. (2025) · Plants · doi:10.3390/plants14060889 · Cited by 18
“Maher et al. [56,57] reported a groundbreaking approach where, after removing the original meris-tematic tissue in tobacco ( Nicotiana benthamiana ), Agrobacterium was injected at the cut site to co-express the GE system and DRs.”
Pars Pro Toto: Every Single Cell Matters
Christiaens, F., Canher, B., Lanssens, F., Bisht, A., Stael, S., Veylder, L. D., & Heyman, J. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.656825 · Cited by 14
“The knowledge concerning this de novo shoot formation following callus induction was translated into an elegant tool allowing the generation of CRISPR-edited plants from tobacco leaves (Maher et al., 2020).”
BackgroundEnhancing HR Frequency for Precise Genome Editing in Plants
Chen, H., Neubauer, M., & Wang, J. P. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.883421 · Cited by 13
Development of plant cytosine base editors with the Cas12a system
Wang, H., Liang, J., Chen, L., Deng, B., Gu, D., Liu, X., Jin, S., Xu, R., Qin, R., Zhu, Y., et al. (2023) · The Crop Journal · doi:10.1016/j.cj.2023.03.002 · Cited by 13
Applications of CRISPR/Cas9 Technology in Ornamental Plants
Liu, H., Wang, Z., Liu, Y., Wei, Y., Hu, Z., Wu, X., Zheng, C., & Wang, C. (2023) · Plant Molecular Biology Reporter · doi:10.1007/s11105-023-01417-2 · Cited by 13
“Some studies have shown that de novo induction of gene editing meristems without tissue culture is expected to solve the drawbacks of traditional transformation methods (Maher et al. 2020).”
Powdery Mildew of Bigleaf Hydrangea: Biology, Control, and Breeding Strategies for Resistance
Jennings, C., Baysal-Gurel, F., & Alexander, L. W. (2024) · Horticulturae · doi:10.3390/horticulturae10030216 · Cited by 16
“New genomic tools for bigleaf hydrangea will enable modern breeding strategies that can effectively be used to produce a bigleaf hydrangea cultivar with durable powdery mildew resistance.”
BackgroundCRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future
Zhang, F., Neik, T. X., Thomas, W. J. W., & Batley, J. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24108623 · Cited by 17
“Gene-edited shoots are induced, which eventually grow into gene-edited plants [156].”
BackgroundUsing precision phenotyping to inform de novo domestication
Fernie, A. R., Alseekh, S., Liu, J., & Yan, J. (2021) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiab160 · Cited by 13
“The second is the recent finding by the group of Dan Voytas that gene-edited dicots can be generated via de novo meristem induction (Maher et al., 2020).”
BackgroundNano-assisted delivery tools for plant genetic engineering: a review on recent developments
Kumar, P., Rajput, V. D., Singh, A. K., Agrawal, S., Das, R., Minkina, T., Shukla, P. K., Wong, M. H., Kaushik, A. K., Albukhaty, S., et al. (2024) · Environmental Science and Pollution Research · doi:10.1007/s11356-024-35806-1 · Cited by 12
“The Bbm and Wus2 genes’ ability to increase transfection efficiency and encourage crop regeneration is one potential remedy (Lowe et al. 2018; Maher et al. 2020).”
Liu, L., Shu, Y., Wang, Y., Liu, M., Xu, S., Lu, X., Zhang, Y., Yu, L., Tao, Z., Wang, J., et al. (2025) · Horticulture Research · doi:10.1093/hr/uhaf117 · Cited by 12
“For instance, strong upregulation of ZmWUS2 has been found to be less effective in promoting de novo meristem induction in tobacco and leaf-based regeneration in monocots compared to mild upregulation [19, 20].”
A graft that crafts nontransgenic and genome-edited plants
Awan, M. J. A., Aslam, M. Q., Naqvi, R. Z., Amin, I. U., & Mansoor, S. (2023) · Trends in Plant Science · doi:10.1016/j.tplants.2023.03.020 · Cited by 12
Role of Strigolactones in the Host Specificity of Broomrapes and Witchweeds
Huizinga, S., & Bouwmeester, H. J. (2023) · Plant and Cell Physiology · doi:10.1093/pcp/pcad058 · Cited by 12
“However, only last year researchers managed to regenerate fertile plants (Maher et al. 2020, Nelson 2021).”
BackgroundYakovleva, D. V., Efremova, E. P., Smirnov, K. V., Simonova, V. Y., Konstantinov, Z. S., Tvorogova, V. E., & Lutova, L. A. (2024) · Plants · doi:10.3390/plants13020223 · Cited by 12
Morey, K. J., & Khakhar, A. (2024) · New Phytologist · doi:10.1111/nph.19650 · Cited by 12
Zhang, D., Li, Z., Htwe, Y. M., Shi, P., Wei, X., Nie, H., Nin, J., Wu, L., Khan, F. S., Yu, Q., et al. (2024) · Industrial Crops and Products · doi:10.1016/j.indcrop.2024.118338 · Cited by 12
Li, Z., Qian, W., Qiu, S., Wang, W., Jiang, M., Hu, X., Huang, H., & Lin, E. (2024) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2024.108570 · Cited by 12
Eliminating tissue culture from plant gene editing in the near future: A wish or reality?
Babaeianjelodar, N., Trivedi, J., & Uhde‐Stone, C. (2025) · Current Plant Biology · doi:10.1016/j.cpb.2025.100433 · Cited by 12
Tan, J., Wang, Y., Chen, S., Lin, Z., Zhao, Y., Xue, Y., Luo, Y., Liu, Y., & Zhu, Q. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23031588 · Cited by 12
“Genetic modification of meristem (SAM) allows the transmission of modifications to flowers and seeds, and ultimately, to future generations [20].”
BackgroundInfluentialApplications and challenges of harnessing genome editing in oilseed crops
Vaikuntapu, P. R., & Kumar, V. D. (2023) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-022-00821-1 · Cited by 12
“…particle bombardment delivery of CRISPR/Cas reagents (Demirer et al. 2020; Banakar et al. 2020), de-novo meristematic microinjection of developmental regulators with gene-editing reagents (Maher et al. 2020), use of viral vectors (Ellison et al. 2020) and CRISPR-combo system (Pan et al. 2022).”
Nagle, M. F., Yuan, J., Kaur, D., Ma, C., Peremyslova, E., Jiang, Y., de Rivera, A. N., Jawdy, S. S., Chen, J., Feng, K., et al. (2024) · G3 Genes Genomes Genetics · doi:10.1093/g3journal/jkae026 · Cited by 12
“…spp. (Deng et al. 2009; Liu et al. 2018; Pan et al. 2022 ), in many cases with the same MRs or their homologs proving effective, and suggesting at least some level of mechanistic conservation across both in vitro and in planta path - ways (Deng et al. 2009; Maher et al. 2020; Lian et al. 2022).”
BackgroundMajumder, S., Sarkar, C., Datta, K., & Datta, S. K. (2020) · Industrial Crops and Products · doi:10.1016/j.indcrop.2020.112620 · Cited by 12
Liu, Y., Liu, S., Deng, Y., You, C., Zhang, W., Zhou, J., Chen, X., Gao, L., & Tang, Y. (2020) · Environmental and Experimental Botany · doi:10.1016/j.envexpbot.2020.104207 · Cited by 12
Multigene engineering in plants: Technologies, applications, and future prospects
Rajput, R., Boone, B. A., Mandlik, R., Islam, M. T., Qi, Y., Wang, J., Barrangou, R., Sozzani, R., Eckert, C. A., Tuskan, G. A., et al. (2025) · Biotechnology Advances · doi:10.1016/j.biotechadv.2025.108697 · Cited by 11
Acha, G., Vergara, R., Muñoz, M., Mora, R., Aguirre, C., Muñoz, M., Kalazich, J., & Humberto Prieto, E. (2021) · Plants · doi:10.3390/plants10091882 · Cited by 11
“However, success in protoplast protocols is far from routine in the current plant tissue culture state of the art, and regular gene transfer procedures, based mostly on regeneration protocols from multicellular explants such as leaves, internodes, and somatic embryos, represent a conditioning factor for gene editing w…”
BackgroundThe Secrets of Meristems Initiation: Axillary Meristem Initiation and Floral Meristem Initiation
Yang, Q., Yuan, C., Cong, T., & Zhang, Q. (2023) · Plants · doi:10.3390/plants12091879 · Cited by 11
“…role in maintaining the meris-tematic ability of the AM or FM [7], and, based on the totipotency of plant cells [145], the ectopic expression of specific DRs in leaf cells can change their developmental fate, re-induce meristem, and ultimately obtain transgenic plants without tissue culture [8].”
BackgroundInfluentialZhang, X., Liang, S., Luo, B., Zhou, Z., Bao, J., Fang, R., Wang, F., Song, X., Liao, Z., Chen, G. J., et al. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241311190 · Cited by 11
“In the case of tobacco plants transfected by ZmWus2, developmental abnormalities like curled leaves were observed [16].”
BackgroundDirected mutagenesis in plants through genome editing using guide RNA library
Dutta, S., Paul, D., Das, A., Kumar, S., Roy, A., Barua, A., & Chinnusamy, V. (2024) · The Nucleus · doi:10.1007/s13237-024-00481-7 · Cited by 11
Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies
Wittmer, J., & Heidstra, R. (2024) · Journal of Experimental Botany · doi:10.1093/jxb/erae264 · Cited by 11
Cell-penetrating peptide: A powerful delivery tool for DNA-free crop genome editing
Wu, H., Zhang, K., Zhang, Z., Wang, J., Jia, P., Cong, L., Li, J., Duan, Y., Ke, F., Zhang, F., et al. (2022) · Plant Science · doi:10.1016/j.plantsci.2022.111436 · Cited by 11
Genome editing of medicinal plants: Advances, challenges, and prospects
Chen, W., Shi, Y., Lv, Z., & Chen, W. (2026) · Journal of Integrative Plant Biology · doi:10.1111/jipb.70110 · Cited by 10
Engineering Conditional Transgene Expression in Nicotiana benthamiana
Garcia‐Perez, E., Vázquez‐Vilar, M., & Orzáez, D. (2025) · Plant Biotechnology Journal · doi:10.1111/pbi.70350 · Cited by 10
“However, innovations, such as the use of morphogenic regulator genes (Cody et al. 2017; Gordon-Kamm et al. 2019) and transformation of embryonic axes or meristems (Ganguly et al. 2020; Maher et al. 2020), have significantly accelerated this process.”
Alok, A., Raman, V., D’Agostino, L. W., Kshetry, A. O., Mohan, K., Wang, C., Gunapati, S., Stupar, R. M., Patil, G. B., & Zhang, F. (2025) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiaf640 · Cited by 10
Tackling Lentil Biotic Stresses in the Genomic Era
de la Vega, M. P., Garcı́a, P., Gutierrez-Gonzalez, J. J., & de Miera, L. E. S. (2022) · Springer eBooks · doi:10.1007/978-3-030-91043-3_5 · Cited by 10
Unlocking Nature’s Clock: CRISPR Technology in Flowering Time Engineering
Hodaei, A., & Werbrouck, S. (2023) · Plants · doi:10.3390/plants12234020 · Cited by 10
“Recent advances include improvements in delivery using improved Agrobacterium strains [208,209], viral delivery [210], improvements in regeneration using morphogenic regulators [211,212], and new approaches to use multiplex gene editing to study many genes simultaneously [213,214].”
BackgroundDebernardi, J. M., Tricoli, D. M., Ercoli, M. F., Hayta, Ş., Ronald, P. C., Palatnik, J. F., & Dubcovsky, J. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.08.23.263905 · Cited by 10
Barragán‐Borrero, V., Lopes, A. D. S., Batista, E. D. R., Höfer, M., Elias, R., Chakraborty, A., Ponce-Mañe, A., Descombes, C., Diezma‐Navas, L., Petraki, L., et al. (2026) · New Phytologist · doi:10.1111/nph.70919 · Cited by 9
“…of GRF-GIF chimeric morphogenic regulators, shown to significantly boost transformation, editing, and regeneration efficiencies in plants (Debernardi et al ., 2020; Maher et al ., 2020; Vandeputte et al ., 2024; Swinnen et al ., 2025), could further boost these processes in SP162 and other genotypes.”
Genetic transformation and gene delivery strategies in Carica papaya L.
Tuo, D., Ma, C., Yan, P., Kong, H., Zhou, P., Guo, A., Zhao, H., & Shen, W. (2023) · Tropical Plants · doi:10.48130/tp-2023-0005 · Cited by 9
Bulle, M., Sheri, V., Aileni, M., & Zhang, B. (2023) · Plants · doi:10.3390/plants12193448 · Cited by 10
“…Baby Boom ( BBM ), and Wuschel2 ( WUS2 ), has been shown to improve plant regeneration, thus, resulting in an increase in nuclear transformation efficiency [104–106], but their effect on plastid in vitro regeneration efficiency remains to be demonstrated in recalcitrant species, such as chili pepper.”
Novel Delivery Methods for CRISPR-Based Plant Genome Editing
Prestwich, B. D., Cardi, T., Bakhsh, A., Nicolia, A., & Bhati, K. K. (2023) · Springer eBooks · doi:10.1007/978-3-031-46150-7_3 · Cited by 9
Jin, K., Tian, N., Ferreira, J., Sandhu, D., Xiao, L., Gu, M., Luo, Y., Zhang, X., Liu, G., Liu, Z., et al. (2022) · Biomolecules · doi:10.3390/biom12050688 · Cited by 9
“PMKV060 was donated by Daniel Voytas [36] (Addgene plasmid #133315; (accessed on 16 January 2020) http://n2t.net/addgene:133315; RRID: Addgene_133315).”
MethodReducing costs, improving profits: A low-cost culture media for woody plants micropropagation
Castro-Camba, R., Vielba, J. M., Covelo, P., Vidal, N., & Sánchez, C. (2023) · Journal of Forest Science · doi:10.17221/56/2023-jfs · Cited by 8
Metadata
- Authors
- Maher, M. F., Nasti, R. A., Vollbrecht, M., Starker, C. G., Clark, M. D., & Voytas, D. F.
- Affiliations
- University of Minnesota (US)
- Corresponding author
- Voytas, Daniel F.
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2019-12-16
- Volume
- 38
- Issue
- 1
- Pages
- 84-89
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC6954279
- OpenAlex
- W2995711654
- Cited by
- 541
- References
- 27
- Citations by year
- 2026: 64 · 2025: 86 · 2024: 68 · 2023: 78 · 2022: 94 · 2021: 93 · 2020: 55 · 2019: 2
- FWCI
- 20.08
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (T32-GM008347)
- Hackett Fund of the University of Minnesota TechAccel
- TechAccel
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- Cas9, single guide RNA, somatic cells, dicotyledonous plants, transgenerational inheritance, developmental regulators, epigenome, genome editing
- MeSH terms
- Gene Editing, CRISPR-Associated Protein 9, Base Sequence, Mutation, Plant Proteins, Soil, Nicotiana, Meristem, Plant Shoots, Transgenes, Plants, Genetically Modified, Seedlings
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
