Cited by 541CRISPR and Genetic EngineeringPlant tissue culture and regenerationPlant Molecular Biology Research

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Plant gene editing through de novo induction of meristems

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Maher, Michael F., Nasti, Ryan A., Vollbrecht, Macy, Starker, Colby G., Clark, Matthew D., Voytas, Daniel F.·2020·Nature Biotechnology

DOI 10.1038/s41587-019-0337-2PMC PMC6954279

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Abstract

Plant gene editing is typically performed by delivering reagents such as Cas9 and single guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole plants by exposure to various hormones. The creation of edited plants through tissue culture is often inefficient, time-consuming, works for only limited species and genotypes, and causes unintended changes to the genome and epigenome. Here we report two methods to generate gene-edited dicotyledonous plants through de novo meristem induction. Developmental regulators and gene-editing reagents are delivered to somatic cells of whole plants. This induces meristems that produce shoots with targeted DNA modifications, and gene edits are transmitted to the next generation. The de novo induction of gene-edited meristems sidesteps the need for tissue culture and promises to overcome a bottleneck in plant gene editing.

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Authors: Maher, M. F., Nasti, R. A., Vollbrecht, M., Starker, C. G., Clark, M. D., & Voytas, D. F.
Affiliations: University of Minnesota (US)
Corresponding author: Voytas, Daniel F.
ORCID: Maher, Michael F.
Nasti, Ryan A.
Vollbrecht, Macy
Starker, Colby G.
Clark, Matthew D.
Voytas, Daniel F.
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2019-12-16
Volume: 38
Issue: 1
Pages: 84-89
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/s41587-019-0337-2
PMC: PMC6954279
OpenAlex: W2995711654
Cited by: 541
References: 27
Citations by year: 2026: 64 · 2025: 86 · 2024: 68 · 2023: 78 · 2022: 94 · 2021: 93 · 2020: 55 · 2019: 2
FWCI: 20.08
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (T32-GM008347)
Hackett Fund of the University of Minnesota TechAccel
TechAccel
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords: Cas9, single guide RNA, somatic cells, dicotyledonous plants, transgenerational inheritance, developmental regulators, epigenome, genome editing
MeSH terms: Gene Editing, CRISPR-Associated Protein 9, Base Sequence, Mutation, Plant Proteins, Soil, Nicotiana, Meristem, Plant Shoots, Transgenes, Plants, Genetically Modified, Seedlings

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Plant gene editing through de novo induction of meristems

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