The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Prime genome editing in rice and wheat
Abstract
Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.
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In the citing paper
“Prime editing allows for all types of desired base substitutions, small insertions up to 44 bp and deletions up to 88 bp at selected target sites with the use of CRSIPR/Cas9 nickase-reverse transcriptase fusions (Anzalone et al., 2019;Lin et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Lin et al., 2020
In the citing paper
“Plant prime-editing systems have been used in rice and wheat, but not in barley (Lin et al., 2020;Xu et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Lin et al., 2020
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Cites
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437
Self-citationSearch-and-replace genome editing without double-strand breaks or donor DNA
Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767
Self-citationProgrammable base editing of A•T to G•C in genomic DNA without DNA cleavage
Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017) · Nature · doi:10.1038/nature24644 · Cited by 4,184
Self-citationWang, Y., Cheng, X., Shan, Q., Zhang, Y., Liu, J., Gao, C., & Qiu, J.-L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2969 · Cited by 2,072
Self-citationTargeted genome modification of crop plants using a CRISPR-Cas system
Shan, Q., Wang, Y., Li, J., Zhang, Y., Chen, K., Liang, Z., Zhang, K., Liu, J., Xi, J. J., Qiu, J.-L., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2650 · Cited by 1,810
Self-citationGenomic variation in 3,010 diverse accessions of Asian cultivated rice
Wang, W., Mauleon, R., Hu, Z., Chebotarov, D., Tai, S., Wu, Z., Li, M., Zheng, T., Fuentes, R. R., Zhang, F., et al. (2018) · Nature · doi:10.1038/s41586-018-0063-9 · Cited by 1,766
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture
Chen, K., Wang, Y., Zhang, R., Zhang, H., & Gao, C. (2019) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050718-100049 · Cited by 1,696
Self-citationA CRISPR/Cas9 toolkit for multiplex genome editing in plants
Xing, H.-L., Dong, L., Wang, Z.-P., Zhang, H.-Y., Han, C.-Y., Liu, B., Wang, X.-C., & Chen, Q.-J. (2014) · BMC Plant Biology · doi:10.1186/s12870-014-0327-y · Cited by 1,684
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems
Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, K. Y., et al. (2016) · Science · doi:10.1126/science.aaf8729 · Cited by 1,349
Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion
Zong, Y., Wang, Y., Li, C., Zhang, R., Chen, K., Ran, Y., Qiu, J., Wang, D., & Gao, C. (2017) · Nature Biotechnology · doi:10.1038/nbt.3811 · Cited by 896
Self-citationSvitashev, S. K., Young, J. K., Schwartz, C., Gao, H., Falco, S. C., & Cigan, A. M. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.15.00793 · Cited by 758
Kim, Y. B., Komor, A. C., Levy, J. M., Packer, M. S., Zhao, K. T., & Liu, D. R. (2017) · Nature Biotechnology · doi:10.1038/nbt.3803 · Cited by 758
Self-citationA Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants
Čermák, T., Curtin, S. J., Gil‐Humanes, J., Čegan, R., Kono, T. J. Y., Konečná, E., Belanto, J. J., Starker, C. G., Mathre, J. W., Greenstein, R. L., et al. (2017) · The Plant Cell · doi:10.1105/tpc.16.00922 · Cited by 668
Gao, Y., & Zhao, Y. (2013) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12152 · Cited by 574
Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Li, C., Zong, Y., Wang, Y., Jin, S., Zhang, D., Song, Q., Zhang, R., & Gao, C. (2018) · Genome biology · doi:10.1186/s13059-018-1443-z · Cited by 507
Self-citationPrecision genome engineering and agriculture: opportunities and regulatory challenges
Voytas, D. F., & Gao, C. (2014) · PLoS Biology · doi:10.1371/journal.pbio.1001877 · Cited by 458
Self-citationEfficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A
Zong, Y., Song, Q., Li, C., Jin, S., Zhang, D., Wang, Y., Qiu, J., & Gao, C. (2018) · Nature Biotechnology · doi:10.1038/nbt.4261 · Cited by 440
Self-citationRapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs
Shan, Q., Wang, Y., Chen, K., Liang, Z., Li, J., Zhang, Y., Zhang, K., Liu, J., Voytas, D. F., Zheng, X., et al. (2013) · Molecular Plant · doi:10.1093/mp/sss162 · Cited by 288
Self-citationGene targeting in plants: 25 years later
Puchta, H., & Fauser, F. (2013) · The International Journal of Developmental Biology · doi:10.1387/ijdb.130194hp · Cited by 175
Current and future editing reagent delivery systems for plant genome editing
Ran, Y., Liang, Z., & Gao, C. (2017) · Science China Life Sciences · doi:10.1007/s11427-017-9022-1 · Cited by 163
Self-citationLim, D., & Maas, W. K. (1989) · Cell · doi:10.1016/0092-8674(89)90693-4 · Cited by 149
Teleseismic S wave microseisms
Nishida, K., & Takagi, R. (2016) · Science · doi:10.1126/science.aaf7573 · Cited by 125
Plant, Á. L., Covey, S. N., & Grierson, D. S. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.23.8305 · Cited by 40
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Self-citationGenome engineering for crop improvement and future agriculture
Gao, C. (2021) · Cell · doi:10.1016/j.cell.2021.01.005 · Cited by 1,070
“…RT template in the pegRNA, using other RTs, processing the pegRNAs with a ribozyme, raising the culture temperature to favor reverse transcription (Lin et al., 2020), using enhanced promoters for pegRNA expression (Jiang et al., 2020), and enriching for transformed cells (Xu et al., 2020a, 2020b).”
InfluentialSelf-citationApplications of CRISPR–Cas in agriculture and plant biotechnology
Zhu, H., Li, C., & Gao, C. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00288-9 · Cited by 870
Self-citationEnhanced prime editing systems by manipulating cellular determinants of editing outcomes
Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C.-D., Nelson, J. W., Newby, G. A., Şahin, M., et al. (2021) · Cell · doi:10.1016/j.cell.2021.09.018 · Cited by 841
“, 2021), rice and wheat (Lin et al., 2020), zebrafish (Petri et al.”
BackgroundSelf-citationPrime editing for precise and highly versatile genome manipulation
Chen, P. J., & Liu, D. R. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-022-00541-1 · Cited by 644
Self-citation
Metadata
- Authors
- Lin, Q., Zong, Y., Xue, C., Wang, S., Jin, S., Zhu, Z., Wang, Y., Anzalone, A. V., Raguram, A., Doman, J. L., Liu, D. R., & Gao, C.
- Affiliations
- Chinese Academy of Sciences (CN)
- Broad Institute (US)
- Corresponding author
- Gao, Caixia
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2020-03-16
- Volume
- 38
- Issue
- 5
- Pages
- 582-585
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W3012016445
- Semantic Scholar
- 8cfc62e789552485d639d0542c1caaaf730db03d
- Cited by
- 873
- References
- 23
- Citations by year
- 2026: 105 · 2025: 118 · 2024: 106 · 2023: 135 · 2022: 146 · 2021: 175 · 2020: 86
- FWCI
- 44.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- National Human Genome Research Institute (RM1HG009490)
- National Institute of General Medical Sciences (R35GM118062)
- National Institute of Allergy and Infectious Diseases (U01AI142756)
- National Institute of Biomedical Imaging and Bioengineering (R01EB022376)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- prime editing, CRISPR/Cas9 nickase, reverse transcriptase, pegRNA, plant genome editing, rice, wheat, protoplast transformation, point mutations, indels, codon optimization, promoter optimization, plant regeneration
- MeSH terms
- Gene Editing, Deoxyribonuclease I, Oryza, Triticum, Genome, Plant, CRISPR-Cas Systems
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