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Cited by 12CRISPR and Genetic EngineeringBacteriophages and microbial interactionsBacterial Genetics and Biotechnology

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Cas1 mediates the interference stage in a phage-encoded CRISPR–Cas system

Open access
Zhang, Laixing, Wang, Hao, Zeng, Jianwei, Cao, Xueli, Gao, Zhengyu, Liu, Zihe, Li, Feixue, Wang, Jiawei, Zhang, Yi, Yang, Maojun, Feng, Yue·2024·Nature Chemical Biology
DOI 10.1038/s41589-024-01659-5

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Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems are prokaryotic adaptive immune systems against invading phages and other mobile genetic elements. Notably, some phages, including the Vibrio cholerae-infecting ICP1 (International Center for Diarrheal Disease Research, Bangladesh cholera phage 1), harbor CRISPR-Cas systems to counteract host defenses. Nevertheless, ICP1 Cas8f lacks the helical bundle domain essential for recruitment of helicase-nuclease Cas2/3 during target DNA cleavage and how this system accomplishes the interference stage remains unknown. Here, we found that Cas1, a highly conserved component known to exclusively work in the adaptation stage, also mediates the interference stage through connecting Cas2/3 to the DNA-bound CRISPR-associated complex for antiviral defense (Cascade; CRISPR system yersinia, Csy) of the ICP1 CRISPR-Cas system. A series of structures of Csy, Csy-dsDNA (double-stranded DNA), Cas1-Cas2/3 and Csy-dsDNA-Cas1-Cas2/3 complexes reveal the whole process of Cas1-mediated target DNA cleavage by the ICP1 CRISPR-Cas system. Together, these data support an unprecedented model in which Cas1 mediates the interference stage in a phage-encoded CRISPR-Cas system and the study also sheds light on a unique model of primed adaptation.

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Metadata

Authors
Zhang, L., Wang, H., Zeng, J., Cao, X., Gao, Z., Liu, Z., Li, F., Wang, J., Zhang, Y., Yang, M., & Feng, Y.
Affiliations
  • Center for Life Sciences (CN)
  • State Key Laboratory of Chemical Resource Engineering (CN)
  • Southern University of Science and Technology (CN)
Corresponding author
  • Zhang, Yi
  • Yang, Maojun
  • Feng, Yue
ORCID
Venue
Nature Chemical Biology
Publisher
Springer Science and Business Media LLC
Published
2024-07-08
Volume
20
Issue
11
Pages
1471-1481
ISSN
  • 1552-4450 (ISSN-L)
  • 1552-4469
DOI
10.1038/s41589-024-01659-5
OpenAlex
W4400422905
Cited by
12
References
3
Citations by year
2026: 5 · 2025: 7
FWCI
1.64
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Chemical Biology (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • Tsinghua University
  • Southern University of Science and Technology
  • National Key Research and Development Program of China
  • Institute of Microbiology, Chinese Academy of Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
CRISPR-Cas, Cas1, Vibrio cholerae, cascades, phage defense, adaptive immunity
MeSH terms
Bacteriophages, DNA, Vibrio cholerae, CRISPR-Cas Systems, CRISPR-Associated Proteins

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