An embedded lipid in the multidrug transporter LmrP suggests a mechanism for polyspecificity

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Evidence for a trap-and-flip mechanism in a proton-dependent lipid transporter

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Investigating the Lipid Selectivity of Membrane Proteins in Heterogeneous Nanodiscs

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Structures of the Mycobacterium tuberculosis efflux pump EfpA reveal the mechanisms of transport and inhibition

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Nanodiscs and mass spectrometry: Making membranes fly

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Interaction of Hoechst 33342 with POPC Membranes at Different pH Values

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Major facilitator superfamily efflux pumps in human pathogens: Role in multidrug resistance and beyond

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Cryo‐EM structure of antibacterial efflux transporter QacA from Staphylococcus aureus reveals a novel extracellular loop with allosteric role

Majumder, P., Ahmed, S., Ahuja, P., Athreya, A., Ranjan, R. D., & Penmatsa, A. (2023) · The EMBO Journal · doi:10.15252/embj.2023113418 · Cited by 15 · see citing works

HDX-guided EPR spectroscopy to interrogate membrane protein dynamics

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Structural Insights into the Binding and Degradation Mechanisms of Protoporphyrin IX by the Translocator Protein TSPO

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Role of environmental specificity in CASP results

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Complexities of a protonatable substrate in measurements of Hoechst 33342 transport by multidrug transporter LmrP

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Conserved binding site in the N-lobe of prokaryotic MATE transporters suggests a role for Na+ in ion-coupled drug efflux

Castellano, S., Claxton, D. P., Ficici, E., Kusakizako, T., Stix, R., Zhou, W., Nureki, O., Mchaourab, H. S., & Faraldo‐Gómez, J. D. (2021) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2021.100262 · Cited by 11 · see citing works

Assessing the binding properties of <scp>CASP14</scp> targets and models

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Mutagenesis and functional analysis of SotB: A multidrug transporter of the major facilitator superfamily from Escherichia coli

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Electron paramagnetic resonance spectroscopy on G-protein-coupled receptors: Adopting strategies from related model systems

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Drug-bound outward-facing conformation of a heterodimeric ABC exporter suggests a putative mechanism of drug translocation

Tang, Q., Sinclair, M., Bisignano, P., Zhang, Y., Tajkhorshid, E., & Mchaourab, H. S. (2025) · Nature Communications · doi:10.1038/s41467-025-65318-6 · Cited by 8 · see citing works

Inverse PCR-based detection reveal novel mobile genetic elements and their associated genes in the human oral metagenome

Tansirichaiya, S., Winje, E., Wigand, J., & Al‐Haroni, M. (2022) · BMC Oral Health · doi:10.1186/s12903-022-02209-y · Cited by 6 · see citing works

Coupling the role of lipids to the conformational dynamics of the ABC transporter P-glycoprotein

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DEERefiner-assisted structural refinement using pulsed dipolar spectroscopy: a study on multidrug transporter LmrP

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Multidrug resistance and major facilitator superfamily antimicrobial efflux pumps of the ESKAPEE pathogen Staphylococcus aureus

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The conserved lysine residue in transmembrane helix 5 is pivotal for the cytoplasmic gating of the L- amino acid transporters

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Understanding, inhibiting, and engineering membrane transporters with high-throughput mutational screens

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Structural mass spectrometry approaches to understand multidrug efflux systems

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Drug-bound and -free outward-facing structures of a multidrug ABC exporter point to a swing mechanismPreprint

Chaptal, V., Zampieri, V., Wiseman, B., Orelle, C., Martin, J., Nguyen, K.-A., Magnard, S., Gobet, A., Cesare, M. D., Javed, W., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.03.12.435132 · Cited by 4 · see citing works

Modeling Protein Conformations by Guiding AlphaFold2 with Distance Distributions. Application to Double Electron Electron Resonance (DEER) SpectroscopyPreprint

Wu, T.-Q., Stein, R. A., Kao, T.-Y., Brown, B., & Mchaourab, H. S. (2024) · bioRxiv · doi:10.1101/2024.10.30.621127 · Cited by 3 · see citing works

Diverse regulation of functional dimerization of a sugar transporter by different interfacial lipids

Zhang, Y., Zhao, W., Duan, M., Shen, Y., Li, J., Xie, H., Tong, Q., Zhao, Y., & Yang, J. (2026) · Nature Communications · doi:10.1038/s41467-026-69804-3 · Cited by 3 · see citing works

Darobactin B Stabilises a Lateral‐Closed Conformation of the BAM Complex in E. coli Cells

Haysom, S. F., Machin, J. M., Whitehouse, J. M., Horne, J. E., Fenn, K. L., Ma, Y., Mkami, H. E., Böhringer, N., Schäberle, T. F., Ranson, N. A., et al. (2023) · Angewandte Chemie · doi:10.1002/ange.202218783 · Cited by 3 · see citing works

Cryo-EM structure of an elusive pre-transport intermediate of the multidrug ABC transporter BmrCD reveals modes of asymmetric drug bindingPreprint

Thaker, T. M., Mishra, S., Zhou, W., Faraldo‐Gómez, J. D., Mchaourab, H. S., & Tomasiak, T. M. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.03.13.435271 · Cited by 3 · see citing works

Tension mediated mechanical activation and pocket delipidation lead to an analogous MscL statePreprint

Wang, B., Lane, B. J., Kapsalis, C., Ault, J. R., Sobott, F., Mkami, H. E., Calabrese, A. N., Kalli, A. C., & Pliotas, C. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.04.01.438050 · Cited by 3 · see citing works

Spontaneous Suppressors against Debilitating Transmembrane Mutants of CaMdr1 Disclose Novel Interdomain Communication via Signature Motifs of the Major Facilitator Superfamily

Sharma, S., Banerjee, A., Moreno, A., Redhu, A. K., Falson, P., & Prasad, R. (2022) · Journal of Fungi · doi:10.3390/jof8050538 · Cited by 1 · see citing works

Fluctuating hydrogen-bond network of the Hv1 ion channel

Jain, H., Lazaratos, M., Pohl, P., & Bondar, A. (2025) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2025.10.021 · Cited by 1 · see citing works

Streptococcus pyogenes Lineage ST62/emm87: The International Spread of This Potentially Invasive Lineage

Martini, C. L., Silva, D. N. S., Viana, A. S., Planet, P. J., Figueiredo, A. M. S., & Ferreira‐Carvalho, B. T. (2023) · Antibiotics · doi:10.3390/antibiotics12101530 · Cited by 1 · see citing works

Evidence for a ‘trap-and-flip’ mechanism in a proton-dependent lipid transporterPreprint

Lambert, E., Mehdipour, A. R., Schmidt, A., Hummer, G., & Pérez, C. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.08.05.453402 · Cited by 1 · see citing works

CryoEM structure of QacA, an antibacterial efflux transporter from Staphylococcus aureusPreprint

Majumder, P., Ahmed, S., Ahuja, P., Athreya, A., Ranjan, R. D., & Penmatsa, A. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.07.09.499445 · Cited by 1 · see citing works

Proton-driven alternating access in a spinster transporter, an emerging family of broad-specificity efflux pumpsPreprint

Dastvan, R., Rasouli, A., Dehghani‐Ghahnaviyeh, S., Gies, S. L., & Tajkhorshid, E. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.03.03.482761

Faculty Opinions recommendation of Highly accurate protein structure prediction with AlphaFold.

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Faculty Opinions recommendation of Highly accurate protein structure prediction with AlphaFold.

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Conserved Lysine in transmembrane helix 5 is key for the inner gating of the LAT transporter BasCPreprint

Fort, J., Nicolàs-Aragó, A., Maggi, L., Molledo, M. M., Kapiki, D., Zijlstra, N., Bodoy, S., Pardon, E., Steyaert, J., Llorca, Ó., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.03.26.586791

Antibiotic-Specific Conformational Landscapes of a Multidrug TransporterPreprint

Maklad, H. R. M. A., Kache, T., Roth, A., Mamkaeva, M. A., Govaerts, C., Hendrix, J., & Martens, C. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.05.17.654648

Additional file 1 of Inverse PCR-based detection reveal novel mobile genetic elements and their associated genes in the human oral metagenome

Tansirichaiya, S., Winje, E., Wigand, J., & Al‐Haroni, M. (2022) · Figshare · doi:10.6084/m9.figshare.19915446

Conformational dynamics of the MdfA multidrug efflux pump and structural mechanisms underlying substrate efflux: A review

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Miniprotein inhibitors of the Staphylococcus aureus efflux transporter NorAPreprint

Mishra, P., Chazin-Gray, A. M., Lamon, G., Kim, D. E., Baker, D. A., & Traaseth, N. J. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.03.05.709893

Structural basis of drug efflux by the staphylococcal efflux pump QacAPreprint

Jodaitis, L., Sutton, P., Hutchin, A., Dashtbani-Roozbehani, A., Wallant, K. C. ’., Pardon, E., Steyaert, J., Martens, C., O’Mara, M. L., Brown, M. H., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.04.10.717755

FAIRsharing record for: AlphaFold Protein Structure Database

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Structural basis of multidrug transport by LmrP, a secondary multidrug transporter

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A three-dimensional model for the substrate binding domain of the multidrug ATP binding cassette transporter LmrA. Mol Pharmacol 66:1169–1179

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Local conformational plasticity underlies ligand recognition and proton coupling in MFS multidrug transportersPreprint

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Relocation of active site carboxylates in major facilitator superfamily multidrug transporter LmrP reveals plasticity in proton interactions

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A Three-Dimensional Model for the Substrate Binding Domain of the Multidrug ATP Binding Cassette Transporter LmrA

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