The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
Open accessAbstract
Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/Cas9 being a highly precise tool, as we did not detect off-target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.
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Cites
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Self-citationFillatti, J. J., Kiser, J., Rose, R., & Comai, L. (1987) · Nature Biotechnology · doi:10.1038/nbt0787-726 · Cited by 538
Gene-edited CRISPR mushroom escapes US regulation
Waltz, E. (2016) · Nature · doi:10.1038/nature.2016.19754 · Cited by 532
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Self-citationPan, C., Ye, L., Qin, L., Liu, X., He, Y., Wang, J., Chen, L., & Lu, G. (2016) · Scientific Reports · doi:10.1038/srep24765 · Cited by 375
Magical mystery tour: MLO proteins in plant immunity and beyond
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Cited by
Applications of CRISPR–Cas in agriculture and plant biotechnology
Zhu, H., Li, C., & Gao, C. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00288-9 · Cited by 870
The plant immune system: From discovery to deployment
Jones, J. D. G., Staskawicz, B. J., & Dangl, J. L. (2024) · Cell · doi:10.1016/j.cell.2024.03.045 · Cited by 544
Applications and potential of genome editing in crop improvement
Zhang, Y., Massel, K., Godwin, I. D., & Gao, C. (2018) · Genome Biology · doi:10.1186/s13059-018-1586-y · Cited by 437
Panno, S., Davino, S., Caruso, A. G., Bertacca, S., Crnogorac, A., Mandić, A., Noris, E., & Matić, S. (2021) · Agronomy · doi:10.3390/agronomy11112188 · Cited by 382
CRISPR/Cas: A powerful tool for gene function study and crop improvement
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Metadata
- Authors
- Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D., & Kamoun, S.
- Venue
- Scientific Reports
- Published
- 2017-03-28
- Volume
- 7
- Issue
- 1
- Pages
- 482-482
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2603054892
- Cited by
- 757
- References
- 14
- FWCI
- 21.74
- Type
- journal article
- Language
- en
- Open access
- gold
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- Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D., & Kamoun, S. (2017). Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Scientific Reports. https://doi.org/10.1038/s41598-017-00578-x
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