Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea
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Abstract The cochlea is innervated by type I and type II afferent neurons. Type I afferents are myelinated, larger diameter neurons that send a single dendrite to contact a single inner hair cell, whereas unmyelinated type II afferents are fewer in number and receive input from many outer hair cells. This strikingly differentiated innervation pattern strongly suggests specialized functions. Those functions could be investigated with specific genetic markers that enable labeling and manipulating each afferent class without significantly affecting the other. Here three mouse models were characterized and tested for specific labeling of either type I or type II cochlear afferents. Nos1 CreER mice showed selective labeling of type I afferent fibers, Slc6a4-GFP mice labeled type II fibers with a slight preference for the apical cochlea, and Drd2-Cre mice selectively labeled type II afferent neurons nearer the cochlear base. In conjunction with the Th 2A-CreER and CGRPα-EGFP lines described previously for labeling type II fibers, the mouse lines reported here comprise a promising toolkit for genetic manipulations of type I and type II cochlear afferent fibers.
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Type II afferents express neuropeptides such as CGRP, which in somatic C-fibers modulates the activity of immune cells that proliferate after trauma.
“Type II afferents express neuropeptides such as CGRP (37, 38, 54), which in somatic C-fibers (55) modulates the activity of immune cells that proliferate after trauma (56).”
From Damage-evoked signals in cochlear neurons and supporting cells (2024) · cites this paper as [38]
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Prior acoustic trauma alters type II afferent activity in the mouse cochlea
Nowak, N., Wood, M. B., Glowatzki, E., & Fuchs, P. A. (2021) · eneuro · doi:10.1523/eneuro.0383-21.2021 · Cited by 13 · see citing works
“Medial olivocochlear neurons as confirmed by Tuj1 staining showed no appreciable expression of GCaMP6f; but, there was some expression in lateral olivocochlear neurons (LOCs) in Th;GCaMP6f and Drd2;GCaMP6f animals as described previously for tdTomato expression (Fig. 1C; Vyas et al., 2019; Wu et al., 2020).”
BackgroundMethodInfluentialSelf-citationDamage-evoked signals in cochlear neurons and supporting cells
Wood, M. B., Nowak, N., & Fuchs, P. A. (2024) · Frontiers in Neurology · doi:10.3389/fneur.2024.1361747 · Cited by 7 · see citing works
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- Authors
- Vyas, P., Wu, J. S., Jimenez, A., Glowatzki, E., & Fuchs, P. A.
- Affiliations
- Johns Hopkins University (US)
- Corresponding author
- Glowatzki, Elisabeth
- Fuchs, Paul Albert
- Venue
- Scientific Reports
- Published
- 2019-04-03
- Volume
- 9
- Issue
- 1
- Pages
- 5549-5549
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC6447598
- OpenAlex
- W2927955698
- Cited by
- 26
- References
- 65
- Citations by year
- 2026: 6 · 2025: 2 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 6 · 2020: 6 · 2019: 1
- FWCI
- 2.29
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- U.S. Department of Health & Human Services | NIH | National Institute on Deafness and Other Communication Disorders (R01DC006476, R01DC012957, R01DC016559, DC005211)
- The John Mitchell, Jr. Trust, the David M. Rubenstein Fund for Hearing Research and the John E. Bordley Professorship
- Topics
- Life Sciences › Neuroscience › Sensory Systems › Hearing, Cochlea, Tinnitus, Genetics
- Life Sciences › Neuroscience › Neurology › Vestibular and auditory disorders
- Life Sciences › Neuroscience › Cognitive Neuroscience › Hearing Loss and Rehabilitation
- Keywords
- transgenic mouse lines, CreER, serotonin transporter, DRD2, inner hair cells, outer hair cells, cochlear innervation, genetic labeling
- MeSH terms
- Animals, Cochlea, Mice, Inbred C57BL, Mice, Transgenic, Myelin Sheath, Nerve Fibers, Neurons, Afferent, Biomarkers, Receptors, Dopamine D2, Hair Cells, Auditory, Outer, Green Fluorescent Proteins, Serotonin Plasma Membrane Transport Proteins, Nitric Oxide Synthase Type I
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