A rapid smartphone-based lactate dehydrogenase test for neonatal diagnostics at the point of care
Open accessAbstract
Abstract There is a growing recognition of the importance of point-of-care tests (POCTs) for detecting critical neonatal illnesses to reduce the mortality rate in newborns, especially in low-income countries, which account for 98 percent of reported neonatal deaths. Lactate dehydrogenase (LDH) is a marker of cellular damage as a result of hypoxia-ischemia in affected organs. Here, we describe and test a POC LDH test direct from whole blood to provide early indication of serious illness in the neonate. The sample-in-result-out POC platform is specifically designed to meet the needs at resource-limited settings. Plasma is separated from whole blood on filter paper with dried-down reagents for colorimetric reaction, combined with software for analysis using a smartphone. The method was clinically tested in newborns in two different settings. In a clinical cohort of newborns of Stockholm (n = 62) and Hanoi (n = 26), the value of R using Pearson’s correlation test was 0.91 (p < 0.01) and the R2 = 0.83 between the two methods. The mean LDH (±SD) for the reference method vs. the POC-LDH was 551 (±280) U/L and 552 (±249) U/L respectively, indicating the clinical value of LDH values measured in minutes with the POC was comparable with standardized laboratory analyses.
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Equipment for point-of-care LDH analysis is now available.
“Equipment for point-of-care LDH analysis is now available, 135”
From Umbilical cord pH, blood gases, and lactate at birth: normal values, interpretation, and clinical utility. (2023) · cites this paper as [135]
The Results (Pre-clinical phase) describe a working point-of-care LDH system. But the Discussion calls it an early, non-commercially available prototype intended as a possible future diagnostic tool. The paper therefore supports the existence of POC LDH equipment, not an unqualified implication that it is generally available. (LLM, conf=0.86)
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- Authors
- Halvorsen, C. P., Olson, L., Araújo, A. C., Karlsson, M., Nguyễn, T. T., Khu, D. T. K., Le, H. T. T., Nguyễn, H. T. B., Winbladh, B., & Russom, A.
- Affiliations
- Karolinska Institutet (SE)
- Uppsala University (SE)
- Vietnam National Children's Hospital (VN)
- Science for Life Laboratory (SE)
- Corresponding author
- Russom, Aman
- Venue
- Scientific Reports
- Published
- 2019-06-26
- Volume
- 9
- Issue
- 1
- Pages
- 9301-9301
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC6595069
- OpenAlex
- W2955079455
- Cited by
- 19
- References
- 21
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 2 · 2023: 1 · 2022: 6 · 2021: 1 · 2020: 2 · 2019: 1
- FWCI
- 0.88
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Uppsala University Publications (Uppsala University) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Swedish Foundation for International Cooperation in Research and Higher Education
- Karolinska Institutet
- Sällskapet Barnavård
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal Health and Biochemistry
- Keywords
- lactate dehydrogenase, point-of-care testing, smartphone-based diagnostics, dried blood spots, colorimetric assay, hypoxia-ischemia, neonatal mortality, resource-limited settings, neonates
- MeSH terms
- Point-of-Care Testing, Smartphone, Hypoxia, Calibration, Colorimetry, Fetal Blood, Humans, Infant, Newborn, Ischemia, L-Lactate Dehydrogenase, Prothrombin Time, Reference Standards, Reference Values, Software, Sweden, Reproducibility of Results, Neonatal Screening, Mobile Applications
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