New insights into the role of plasmids from probiotic Lactobacillus pentosus MP-10 in Aloreña table olive brine fermentation
Open accessAbstract
In silico analysis of Lactobacillus pentosus MP-10 plasmids (pLPE-1 to pLPE-5) suggests that plasmid-borne genes mediate the persistence of lactobacilli during olive fermentation and enhance their probiotic properties and their competitiveness in several ecological niches. The role of plasmids in the probiotic activities of L. pentosus MP-10 was investigated by plasmid-curing process which showed that plasmids contribute in increased metal tolerance and the biosequestration of several metals such as iron, aluminium, cobalt, copper, zinc, cadmium and mercury. Statistically significant differences in mucin adhesion were detected between the uncured and the cured L. pentosus MP-10, which possibly relied on a serine-rich adhesin (sraP) gene detected on the pLPE-2 plasmid. However, plasmid curing did not affect their tolerance to gastro-intestinal conditions, neither their growth ability under pre-determined conditions, nor auto-aggregation and pathogen co-aggregation were changed among the cured and uncured L. pentosus MP-10. These findings suggest that L. pentosus MP-10 plasmids play an important role in gastro-intestinal protection due to their attachment to mucin and, thus, preventing several diseases. Furthermore, L. pentosus MP-10 could be used as a bioquencher of metals in the gut, reducing the amount of these potentially toxic elements in humans and animals, food matrices, and environmental bioremediation.
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Many recent works have demonstrated several health benefits of probiotic LAB found in fermented olives.
“Many recent works have demonstrated several health benefits of probiotic LAB found in fermented olives.”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [7]
The Introduction describes fermented olives as a potential source of probiotic lactobacilli, and the Conclusions report probiotic properties of one olive-derived strain, L. pentosus MP-10. But the paper presents intestinal metal detoxification as a potential application; it does not show that many studies have demonstrated several health benefits in people. (LLM, conf=0.86)
Experimental — accuracy not yet measuredThe most studied activities of probiotic LAB from fermented olives are antibacterial, antifungal, anti-adherent, antiproliferative, anticancer, metal detox, and immunostimulant.
“The most studied activities were mainly antibacterial, antifungal, anti-adherent, antiproliferative, anticancer, metal detox, and immunostimulant [7-9].”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [7]
The Introduction mentions antimicrobial activity as a property of Lactobacillus plasmids, and the Conclusions report metal removal by an olive-derived probiotic strain. The paper does not establish that the listed activities are the most studied, or address antifungal, antiproliferative, anticancer, and immunostimulant activities as properties of these probiotics. Page numbers are not available in our copy. (LLM, conf=0.86)
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- Authors
- Abriouel, H., Pérez Montoro, B., de la Fuente Ordoñez, J. J., Lavilla Lerma, L., Knapp, C. W., & Benomar, N.
- Affiliations
- Universidad de Jaén (ES)
- University of Strathclyde (GB)
- Corresponding author
- Abriouel, Hikmate
- Venue
- Scientific Reports
- Published
- 2019-07-29
- Volume
- 9
- Issue
- 1
- Pages
- 10938-10938
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC6662855
- OpenAlex
- W2966690478
- Cited by
- 22
- References
- 43
- Citations by year
- 2025: 6 · 2024: 3 · 2023: 4 · 2022: 5 · 2021: 2 · 2020: 2
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Strathprints: The University of Strathclyde institutional repository (University of Strathclyde) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Ministerio de Economía y Competitividad (AGL2013-43571-P)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus pentosus, plasmids, probiotics, plasmid curing, metal tolerance, mucin adhesion, gastrointestinal tolerance, self-aggregation, metal bioremediation
- MeSH terms
- Lactiplantibacillus pentosus, Fermentation, Genes, Bacterial, Mucins, Plasmids, Adhesins, Bacterial, Metals, Heavy, Probiotics, Olea
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