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Cited by 17Neuroscience and Neuropharmacology ResearchCellular transport and secretionMemory and Neural Mechanisms

Syntaxin-3 is dispensable for basal neurotransmission and synaptic plasticity in postsynaptic hippocampal CA1 neurons

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Shi, Shan, Ma, Ke, Bin, Na-Ryum, Harada, Hidekiyo, Xie, Xiaoyu, Huang, Mengjia, Liu, Haiyu, Lee, Soomin +5 more·2020·Scientific Reports
DOI 10.1038/s41598-019-57388-6PMC PMC6971263
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Abstract

Recent evidence suggests that SNARE fusion machinery play critical roles in postsynaptic neurotransmitter receptor trafficking, which is essential for synaptic plasticity. However, the key SNAREs involved remain highly controversial; syntaxin-3 and syntaxin-4 are leading candidates for the syntaxin isoform underlying postsynaptic plasticity. In a previous study, we showed that pyramidal-neuron specific conditional knockout (cKO) of syntaxin-4 significantly reduces basal transmission, synaptic plasticity and impairs postsynaptic receptor trafficking. However, this does not exclude a role for syntaxin-3 in such processes. Here, we generated and analyzed syntaxin-3 cKO mice. Extracellular field recordings in hippocampal slices showed that syntaxin-3 cKO did not exhibit significant changes in CA1 basal neurotransmission or in paired-pulse ratios. Importantly, there were no observed differences during LTP in comparison to control mice. Syntaxin-3 cKO mice performed similarly as the controls in spatial and contextual learning tasks. Consistent with the minimal effects of syntaxin-3 cKO, syntaxin-3 mRNA level was very low in hippocampal and cortex pyramidal neurons, but strongly expressed in the corpus callosum and caudate axon fibers. Together, our data suggest that syntaxin-3 is dispensable for hippocampal basal neurotransmission and synaptic plasticity, and further supports the notion that syntaxin-4 is the major isoform mediating these processes.

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  • Stx-3 is reported to be required for LTP.

    “On the one hand Stx-3 is reported to be required for LTP (Ref. 334, but see Ref. 339)”

    From Synaptic memory and CaMKII (2023) · cites this paper as [339]

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Cited by

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Authors
Shi, S., Ma, K., Bin, N.-R., Harada, H., Xie, X., Huang, M., Liu, H., Lee, S., Wang, X. F., Adachi, R., Monnier, P. P., Zhang, L., & Sugita, S.
Affiliations
  • University Health Network (CA)
  • The University of Texas MD Anderson Cancer Center (US)
Corresponding author
  • Ma, Ke
  • Sugita, Shuzo
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2020-01-20
Volume
10
Issue
1
Pages
709-709
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-019-57388-6
PMC
PMC6971263
OpenAlex
W3002680483
Cited by
17
References
0
Citations by year
2026: 2 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 2
FWCI
0.92
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada (CGS-D2, 06438)
  • Gouvernement du Canada | Canadian Institutes of Health Research (93665, 130573)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords
syntaxin 3, syntaxin 4, SNARE proteins, synaptic vesicle fusion, synaptic plasticity, long-term potentiation, CA1 pyramidal neurons, conditional knockout mice, paired-pulse ratio, spatial learning, contextual learning, corpus callosum
MeSH terms
Animals, Corpus Callosum, Synaptic Transmission, Neuronal Plasticity, RNA, Messenger, Gene Expression, Long-Term Potentiation, Mice, Knockout, Qa-SNARE Proteins, CA1 Region, Hippocampal, In Vitro Techniques

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