The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum
Open accessAbstract
Extracellular vesicles (EVs) are nano-sized vesicles containing nucleic acid and protein cargo that are released from a multitude of cell types and have gained significant interest as potential diagnostic biomarkers. Human serum is a rich source of readily accessible EVs; however, the separation of EVs from serum proteins and non-EV lipid particles represents a considerable challenge. In this study, we compared the most commonly used isolation techniques, either alone or in combination, for the isolation of EVs from 200 µl of human serum and their separation from non-EV protein and lipid particles present in serum. The size and yield of particles isolated by each method was determined by nanoparticle tracking analysis, with the variation in particle size distribution being used to determine the relative impact of lipoproteins and protein aggregates on the isolated EV population. Purification of EVs from soluble protein was determined by calculating the ratio of EV particle count to protein concentration. Finally, lipoprotein particles co-isolated with EVs was determined by Western blot analysis of lipoprotein markers APOB and APOE. Overall, this study reveals that the choice of EV isolation procedure significantly impacts EV yield from human serum, together with the presence of lipoprotein and protein contaminants.
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Extracellular vesicles: Exosomes, microvesicles, and friends
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Zhou, B., Xu, K., Zheng, X., Chen, T., Wang, J., Song, Y., Shao, Y., & Zheng, S. (2020) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-020-00258-9 · Cited by 685
Modern isolation and separation techniques for extracellular vesicles
Liangsupree, T., Multia, E., & Riekkola, M.-L. (2021) · Journal of Chromatography A · doi:10.1016/j.chroma.2020.461773 · Cited by 512
Jia, Y., Yu, L., Ma, T., Xu, W., Qian, H., Sun, Y., & Shi, H. (2022) · Theranostics · doi:10.7150/thno.74305 · Cited by 391
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs
Yan, H., Wen, Y., Tian, Z., Hart, N., Han, S., Hughes, S. J., & Zeng, Y. (2023) · Nature Biomedical Engineering · doi:10.1038/s41551-023-01033-1 · Cited by 361
Visan, K. S., Lobb, R. J., Ham, S., Lima, L. G., Palma, C., Edna, C. P. Z., Wu, L., Gowda, H., Datta, K. K., Hartel, G., et al. (2022) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12266 · Cited by 308
Metadata
- Authors
- Brennan, K., Martin, K., FitzGerald, S. P., O’Sullivan, J., Wu, Y., Blanco, A., Richardson, C., & Mc Gee, M. M.
- Venue
- Scientific Reports
- Published
- 2020-01-23
- Volume
- 10
- Issue
- 1
- Pages
- 1039-1039
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W3001058124
- Cited by
- 879
- References
- 8
- FWCI
- 32.57
- Type
- journal article
- Language
- en
- Open access
- gold
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- Brennan, K., Martin, K., FitzGerald, S. P., O’Sullivan, J., Wu, Y., Blanco, A., Richardson, C., & Mc Gee, M. M. (2020). A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum. Scientific Reports. https://doi.org/10.1038/s41598-020-57497-7
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