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Cited by 77Wound Healing and TreatmentsHydrogels: synthesis, properties, applicationsDiabetic Foot Ulcer Assessment and Management

Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing

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Shin, Da Yong, Park, Ji-Ung, Choi, Min-Ha, Kim, Sukwha, Kim, Hyoun-Ee, Jeong, Seol-Ha·2020·Scientific Reports
DOI 10.1038/s41598-020-74004-0PMC PMC7546631
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Abstract

Patients with diabetes experience delayed wound healing because of the uncontrolled glucose level in their bloodstream, which leads to impaired function of white blood cells, poor circulation, decreased production and repair of new blood vessels. Treatment using polydeoxyribonucleotide (PDRN), which is a DNA extracted from the sperm cells of salmon, has been introduced to accelerate the healing process of diabetic wounds. To accelerate the wound-healing process, sustained delivery of PDRN is critical. In this study, taking advantage of the non-invasive gelation property of alginate, PDRN was loaded inside the hydrogel (Alg-PDRN). The release behavior of PDRN was altered by controlling the crosslinking density of the Alg hydrogel. The amount of PDRN was the greatest inside the hydrogel with the highest crosslinking density because of the decreased diffusion. However, there was an optimal degree of crosslinking for the effective release of PDRN. In vitro studies using human dermal fibroblasts and diabetes mellitus fibroblasts and an in ovo chorioallantoic membrane assay confirmed that the Alg-PDRN hydrogel effectively induced cell proliferation and expression of angiogenic growth factors and promoted new blood vessel formation. Its effectiveness for accelerated diabetic wound healing was also confirmed in an in-vivo animal experiment using a diabetic mouse model.

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  • Upon enzymatic degradation, PDRN yields a pool of bioavailable nucleosides and nucleotides that can be rapidly reincorporated into cellular processes, promoting tissue regeneration and accelerating wound healing.

    “Upon enzymatic degradation, PDRN yields a pool of bioavailable nucleosides and nucleotides that can be rapidly reincorporated into cellular processes, thereby promoting tissue regeneration and accelerating wound healing [50,75,76,77].”

    From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [50]

    The Introduction says PDRN is degraded by DNA nuclease and links its wound-healing effects to A2A receptors and salvage pathways. The Abstract reports accelerated diabetic wound healing with a PDRN-loaded hydrogel. Neither passage states that degradation yields a bioavailable pool of nucleosides and nucleotides that is rapidly reincorporated into cellular processes, so the claim’s specific mechanism is not supported. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Shin, D. Y., Park, J.-U., Choi, M.-H., Kim, S., Kim, H.-E., & Jeong, S.-H.
Affiliations
Seoul National University (KR)
Corresponding author
Jeong, Seol-Ha
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2020-10-08
Volume
10
Issue
1
Pages
16811-16811
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-020-74004-0
PMC
PMC7546631
OpenAlex
W3092170760
Cited by
77
References
1
Citations by year
2026: 10 · 2025: 21 · 2024: 10 · 2023: 15 · 2022: 10 · 2021: 10 · 2020: 1
FWCI
4.99
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetic Foot Ulcer Assessment and Management
Keywords
polydeoxyribonucleotide, diabetic wound healing, alginate hydrogel, sustained drug release, crosslink density, controlled release, angiogenesis, dermal fibroblasts, chorioallantoic membrane assay, diabetic mouse model, wound healing
MeSH terms
Alginates, Animals, Diabetes Mellitus, Fibroblasts, Humans, Male, Mice, Inbred C57BL, Polydeoxyribonucleotides, Wound Healing, Neovascularization, Physiologic, Hydrogels, Mice

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