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Cited by 97Wound Healing and TreatmentsDermatologic Treatments and ResearchBurn Injury Management and Outcomes

Scar Prevention and Enhanced Wound Healing Induced by Polydeoxyribonucleotide in a Rat Incisional Wound-Healing Model

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Jeong, Woonhyeok, Yang, Chae Eun, Roh, Tai Suk, Kim, Jun Hyung, Lee, Ju Hee, Lee, Won Jai·2017·International Journal of Molecular Sciences
DOI 10.3390/ijms18081698PMC PMC5578088
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Abstract

High-mobility group box protein-1 (HMGB-1) plays a central role in the inflammatory network, and uncontrolled chronic inflammation can lead to excessive scarring. The aim of this study was to evaluate the anti-inflammatory effects of polydeoxyribonucleotide (PDRN) on scar formation. Sprague-Dawley rats (n = 30) underwent dorsal excision of the skin, followed by skin repair. PDRN (8 mg/kg) was administered via intraperitoneal injection for three (PDRN-3 group, n = 8) or seven (PDRN-7 group, n = 8) days, and HMGB-1 was administered via intradermal injection in addition to PDRN treatment for three days (PDRN-3+HMGB-1 group; n = 6). The scar-reducing effects of PDRN were evaluated in the internal scar area and by inflammatory cell counts using histology and immunohistochemistry. Western blot, immunohistochemistry and immunofluorescence assays were performed to observe changes in type I and type III collagen and the expression of HMGB-1 and CD45. Treatment with PDRN significantly reduced the scar area, inflammatory cell infiltration and the number of CD45-positive cells. In addition, the increased expression of HMGB-1 observed in the sham group was significantly reduced after treatment with PDRN. Rats administered HMGB-1 in addition to PDRN exhibited scar areas with inflammatory cell infiltration similar to the sham group, and the collagen synthesis effects of PDRN were reversed. In summary, PDRN exerts anti-inflammatory and collagen synthesis effects via HMGB-1 suppression, preventing scar formation. Thus, we believe that the anti-inflammatory and collagen synthesis effects of PDRN resulted in faster wound healing and decreased scar formation.

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  • Upon enzymatic degradation, PDRN yields a pool of bioavailable nucleosides and nucleotides that can be rapidly reincorporated into cellular processes, promoting tissue regeneration and accelerating wound healing.

    “Upon enzymatic degradation, PDRN yields a pool of bioavailable nucleosides and nucleotides that can be rapidly reincorporated into cellular processes, thereby promoting tissue regeneration and accelerating wound healing [50,75,76,77].”

    From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [75]

    The Discussion (page not provided) reports faster wound healing after PDRN treatment and attributes it to reduced inflammation and increased collagen synthesis. The Introduction describes PDRN as a mixture of nucleotides, but our copy does not say that enzymatic degradation releases bioavailable nucleosides and nucleotides for rapid reincorporation into cellular processes. That proposed mechanism is not supported by this source. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Jeong, W., Yang, C. E., Roh, T. S., Kim, J. H., Lee, J. H., & Lee, W. J.
Affiliations
  • Keimyung University Dongsan Medical Center (KR)
  • Severance Hospital (KR)
  • Department of Plastic and Reconstructive Surgery, School of Medicine & Institute for Medical Science, Keimyung University, Dongsan Medical Center, Daegu 41931, Korea
  • Yonsei University (KR)
Corresponding author
  • Lee, Ju Hee
  • Lee, Won Jai
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2017-08-03
Volume
18
Issue
8
Pages
1698
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms18081698
PMC
PMC5578088
OpenAlex
W2743752524
Cited by
97
References
4
Citations by year
2026: 13 · 2025: 12 · 2024: 11 · 2023: 11 · 2022: 14 · 2021: 9 · 2020: 5 · 2019: 10 · 2018: 8 · 2017: 3
FWCI
6.77
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
Funders
National Research Foundation of Korea (NRF) grant (MEST; No. 2014051295, WJ Lee)
Topics
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Health Sciences › Medicine › Dermatology › Dermatologic Treatments and Research
  • Health Sciences › Medicine › Epidemiology › Burn Injury Management and Outcomes
Keywords
polydeoxyribonucleotide, scar prevention, wound healing, HMGB1, chronic inflammation, anti-inflammatory activity, collagen synthesis, type I collagen, type III collagen, inflammatory cell infiltration, CD45, excision wound
MeSH terms
Animals, Cicatrix, Disease Models, Animal, Male, Polydeoxyribonucleotides, Wound Healing, Wounds and Injuries, Rats, Sprague-Dawley, Collagen Type I, Collagen Type III, HMGB1 Protein, Rats

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