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The effect of smoking on chronic inflammation, immune function and blood cell composition
Open accessAbstract
Smoking is the number one risk factor for cancer mortality but only 15–20% of heavy smokers develop lung cancer. It would, therefore, be of great benefit to identify those at high risk early on so that preventative measures can be initiated. To investigate this, we evaluated the effects of smoking on inflammatory markers, innate and adaptive immune responses to bacterial and viral challenges and blood cell composition. We found that plasma samples from 30 heavy smokers (16 men and 14 women) had significantly higher CRP, fibrinogen, IL-6 and CEA levels than 36 non-smoking controls. Whole blood samples from smokers, incubated for 7 h at 37 °C in the absence of any exogenous stimuli, secreted significantly higher levels of IL-8 and a number of other cytokines/chemokines than non-smokers. When challenged for 7 h with E. coli, whole blood samples from smokers secreted significantly lower levels of many inflammatory cytokines/chemokines. However, when stimulated with HSV-1, significantly higher levels of both PGE 2 and many cytokines/chemokines were secreted from smokers’ blood samples than from controls. In terms of blood cell composition, red blood cells, hematocrits, hemoglobin levels, MCV, MCH, MCHC, Pct and RDW levels were all elevated in smokers, in keeping with their compromised lung capacity. As well, total leukocytes were significantly higher, driven by increases in granulocytes and monocytes. In addition, smokers had lower NK cells and higher Tregs than controls, suggesting that smoking may reduce the ability to kill nascent tumor cells. Importantly, there was substantial person-to person variation amongst smokers with some showing markedly different values from controls and others showing normal levels of many parameters measured, indicating the former may be at significantly higher risk of developing lung cancer.
Overview
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TL;DR
In a cross-sectional comparison, heavy smokers showed elevated chronic inflammation markers (CRP, fibrinogen, IL-6, CEA) and altered blood cell composition (higher granulocytes/monocytes/Tregs, lower NK cells) versus non-smokers. Whole blood from smokers also had higher unstimulated and HSV-1-induced cytokine/PGE2 responses but lower responses to E. coli, with substantial person-to-person variation.
Key findings
- Plasma from 30 heavy smokers had significantly higher CRP (p = 0.046), fibrinogen (p = 0.005), IL-6 (p = 0.049), and CEA (p = 0.0009) than 36 non-smoking controls; PGE2 was not significantly elevated (p = 0.259).
- After 7 h incubation without exogenous stimulus, smokers’ whole blood secreted significantly more IL-8 (p < 0.0001). With HSV-1, smokers’ blood secreted significantly more G-CSF (p = 0.012), IL-8 (p = 0.002), IL-1β (p = 0.004), IFNγ (p = 0.0002), IL-1RA (p = 0.0005), IL-12 (p < 0.0001), TNFα (p = 0.003), VEGF (p < 0.0001), and PGE2. With E. coli, smokers’ blood secreted significantly less IL-1β (p = 0.002), IL-10 (p = 0.0003), IL-6 (p = 0.0004), MIP1α (p = 0.02), and TNFα (p < 0.0001).
- Smokers had significantly higher hematocrit (p = 0.01), hemoglobin (p = 0.0002), MCV/MCH/MCHC (p < 0.05), RDW (p = 0.007), and plateletcrit (p = 0.02), but no significant difference in RBC count (p = 0.212). Total leukocytes, monocytes, and granulocytes were significantly higher (p < 0.0001 each), lymphocytes were not (p = 0.058), NK cells were lower (p = 0.0002), and Tregs were higher; CD4/CD8 ratio did not change.
- There was substantial person-to-person variation among smokers, with some showing markedly different values from controls and others normal levels across many parameters.
Methods
- Cross-sectional comparison of 30 heavy smokers (current smokers smoking ≥ 1 pack/day for at least 20 years; 16 men and 14 women) and 36 healthy never-smoker controls; blood collected at BC Cancer between 8:30 am and 10:00 am.
- Whole blood was incubated for 7 h at 37 °C in 5% oxygen with PBS, E. coli, or HSV-1; plasma cytokines/chemokines were measured by Luminex and MesoScale, and PGE2, fibrinogen, and CRP by ELISA. Blood differential counts used a Coulter Ac•T diff2 analyzer; immunophenotyping used flow cytometry; T cell activation used anti-CD3 + anti-CD28.
- Statistical comparisons used unpaired two-tailed t-tests or Mann–Whitney tests after normality/variance checks, with Pearson correlations; p < 0.05 considered significant.
Limitations
- Stated: the sample size is relatively small, the data set is from a single time point, and it is difficult to determine the relative weight to give different biomarkers; the authors intend to expand the sample and follow participants over time to see who develops lung cancer.
- Evident: the cross-sectional design cannot establish causality or directly predict future lung cancer, and the study reports no cancer outcomes.
- Evident: subgroup analyses are small (e.g., 9 smokers with COPD vs 21 without; sex comparisons), and some in vitro assays gave discordant IFNγ results between whole blood and PBMC cultures.
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0 Valid · 0 Not valid · 41 Not checked · 80 Own results and interpretations
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In the paper
“hematocrits (top right panel, Fig. 5, p = 0.01) and hemoglobin levels (2nd row left panel, Fig. 5, p = 0.0002) were significantly elevated in smokers.”
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Results > Smoking increases the size and hemoglobin level of red blood cells
plainstates an association- Asserted: passes — The authors put it forward as true.
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“there was a trend towards higher levels of IFNγ secretion under both unstimulated and stimulated conditions.”
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Results > Smoking tends to increase IFNγ secretion from activated T cells
hedgedstates an association- Asserted: passes — The authors put it forward as true.
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- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
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“we found a significantly (p = 0.0002) lower proportion of natural killer (NK) cells (i.e., both CD56 bright and dim cells) and higher Tregs (CD4 + CD127low/-CD25 + FoxP3 + cells) (Fig. 7)”
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Results > Smokers have elevated monocytes, granulocytes and Tregs and reduced NK cells
plainstates an association- Asserted: passes — The authors put it forward as true.
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In the paper
“Of note, there was a strong correlation, following E. coli stimulation, between IL-6 and MIP-1α (R = 0.913) levels as well as between IL-1β and TNFα (R = 0.822) levels (Table 3, Supplementary Table S2).”
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Results > Blood from smokers secrete elevated levels of inflammatory cytokines/chemokines when incubated with or without HSV-1 but reduced levels with E. coli
boostedstates an association- Asserted: passes — The authors put it forward as true.
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In the paper
“However, in response to E. coli stimulation this trend was reversed with significantly lower levels of IL-1β (p = 0.002), IL-10 (p = 0.0003), IL-6 (p = 0.0004), MIP1α (p = 0.02) and TNFα (p < 0.0001) in whole blood samples from smokers than from controls (Fig. 3b).”
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Results > Blood from smokers secrete elevated levels of inflammatory cytokines/chemokines when incubated with or without HSV-1 but reduced levels with E. coli
plainstates a cause- Asserted: passes — The authors put it forward as true.
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- Rests on 2 assumptions not shown here.
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Each contradiction between two passages listed here was confirmed by an independent check.
Rated minor: it would not touch a result — a value no result rests on, a p-value that stays on the same side of the significance level, or a conflict that is clerical, resolved elsewhere in the paper, or outside its own findings.
In the paper
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Abstract
“red blood cells, hematocrits, hemoglobin levels, MCV, MCH, MCHC, Pct and RDW levels were all elevated in smokers”
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Results > Smoking increases the size and hemoglobin level of red blood cells
“Figure 5 Smoking increases hematocrits, HgB, MCV, MCH, MCHC, RDW and Pct but not RBC levels.”
Rated minor: it would not touch a result — a value no result rests on, a p-value that stays on the same side of the significance level, or a conflict that is clerical, resolved elsewhere in the paper, or outside its own findings.
In the paper
“| Male % | 33.3% | 55.1% |”
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Results > Smoking is associated with elevated CRP, fibrinogen, IL-6 and CEA but not PGE2 levels
Reported 55.1 · recomputed 53.333333
Rated minor: it would not touch a result — a value no result rests on, a p-value that stays on the same side of the significance level, or a conflict that is clerical, resolved elsewhere in the paper, or outside its own findings.
In the paper
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Discussion > Smoking is associated with elevated CRP, fibrinogen, IL-6 and CEA levels
“in response to HIV-1 stimulation, significantly lower levels of IL-1β.”
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Results > Blood from smokers secrete elevated levels of inflammatory cytokines/chemokines when incubated with or without HSV-1 but reduced levels with E. coli
“To mimic an in vivo bacterial or viral infection, we employed intact E. coli and HSV-1 as in our earlier obesity20and aging studies21.”
Rated minor: it would not touch a result — a value no result rests on, a p-value that stays on the same side of the significance level, or a conflict that is clerical, resolved elsewhere in the paper, or outside its own findings.
In the paper
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Results > Blood from smokers secrete elevated levels of inflammatory cytokines/chemokines when incubated with or without HSV-1 but reduced levels with E. coli
“Whole fresh blood from smokers and controls were diluted tenfold with RPMI and incubated for 4 days at 37 °C in the absence (left panel) or presence (right panel) of anti-CD3 + anti-CD28 antibodies.”
§ The effect of smoking on chronic inflammation, immune function and blood cell composition > Methods > T cell activation
“Fresh whole blood samples collected in heparin were diluted tenfold with RPMI and 90 µl of the diluted blood was incubated in 96-well flat-bottom tissue culture plates in a humidified incubator at 5% CO2, 37ºC for 2 days in the presence or absence of anti-CD3 + anti-CD28.”
Citations
Cites
IL‐6 in inflammation, immunity, and disease
Tanaka, T., Narazaki, M., & Kishimoto, T. (2014) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a016295 · Cited by 5,046
Role of C-Reactive Protein at Sites of Inflammation and Infection
Sproston, N. R., & Ashworth, J. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.00754 · Cited by 3,276
Antiviral Actions of Interferons
Samuel, C. E. (2001) · Clinical Microbiology Reviews · doi:10.1128/cmr.14.4.778-809.2001 · Cited by 2,743
Kishimoto, T. (1989) · Blood · doi:10.1182/blood.v74.1.1.bloodjournal7411 · Cited by 2,068
Ridker, P. M., MacFadyen, J. G., Thuren, T., Everett, B. M., Libby, P., Glynn, R. J., Ridker, P., Lorenzatti, A., Krum, H., Varigos, J., et al. (2017) · The Lancet · doi:10.1016/s0140-6736(17)32247-x · Cited by 1,344
Ridker, P. M. (2016) · Circulation Research · doi:10.1161/circresaha.115.306656 · Cited by 1,002
Global Effects of Smoking, of Quitting, and of Taxing Tobacco
Jha, P., & Peto, R. (2014) · New England Journal of Medicine · doi:10.1056/nejmra1308383 · Cited by 843
Yanbaeva, D. G., Dentener, M. A., Creutzberg, E. C., Wesseling, G., & Wouters, E. F. M. (2007) · Chest · doi:10.1378/chest.06-2179 · Cited by 809
Systemic oxidative stress in asthma, COPD, and smokers
Rahman, I., Morrison, D., Donaldson, K., & MacNee, W. (1996) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.154.4.8887607 · Cited by 774
Cigarette Smoking and Inflammation: Cellular and Molecular Mechanisms
Lee, J., Taneja, V., & Vassallo, R. (2011) · Journal of Dental Research · doi:10.1177/0022034511421200 · Cited by 705
Cited by
Epidemiology and prevention of venous thromboembolism
Lutsey, P. L., & Zakai, N. A. (2022) · Nature Reviews Cardiology · doi:10.1038/s41569-022-00787-6 · Cited by 577
Grosso, G., Laudisio, D., Frias-Toral, E., Barrea, L., Muscogiuri, G., Savastano, S., & Colao, A. (2022) · Nutrients · doi:10.3390/nu14061137 · Cited by 255
Salivary biomarkers: novel noninvasive tools to diagnose chronic inflammation
Dongiovanni, P., Meroni, M., Casati, S., Goldoni, R., Thomaz, D. V., Kehr, N. S., Galimberti, D., FABBRO, M. D., & Tartaglia, G. M. (2023) · International Journal of Oral Science · doi:10.1038/s41368-023-00231-6 · Cited by 234
Cytokines as key players in obesity low grade inflammation and related complications
Uti, D. E., Atangwho, I. J., Omang, W. A., Alum, E. U., Obeten, U. N., Udeozor, P. A., Agada, S. A., Bawa, I., & Ogbu, C. O. (2025) · Obesity Medicine · doi:10.1016/j.obmed.2025.100585 · Cited by 117
Garon, E. B., Lü, S., Goto, Y., Marchi, P. D., Paz-Ares, L. G., Spigel, D. R., Thomas, M., Yang, J. C., Ardizzoni, A., Barlési, F., et al. (2023) · Journal of Clinical Oncology · doi:10.1200/jco.23.00910 · Cited by 90
Self-citationRisk factors for hepatocellular carcinoma: an umbrella review of systematic review and meta-analysis
Wang, J., Qiu, K., Zhou, S., Gan, Y., Jiang, K., Wang, D., & Wang, H. (2025) · Annals of Medicine · doi:10.1080/07853890.2025.2455539 · Cited by 73
Khudhur, Z. O., Smail, S. W., Awla, H. K., Ahmed, G. B., Khdhir, Y. O., Amin, K., & Janson, C. (2025) · Scientific Reports · doi:10.1038/s41598-025-03075-8 · Cited by 48
The Role of Oxidative Stress and Antioxidants in Cardiovascular Comorbidities in COPD
Miklós, Z., & Horváth, I. (2023) · Antioxidants · doi:10.3390/antiox12061196 · Cited by 37
Sørensen, N. V., Orlovska‐Waast, S., Jeppesen, R., Klein‐Petersen, A. W., Christensen, R. H. B., & Benros, M. E. (2022) · Biological Psychiatry · doi:10.1016/j.biopsych.2022.04.002 · Cited by 34
Joharatnam‐Hogan, N., Hatem, D., Cafferty, F. H., Petrucci, G., Cameron, D., Ring, A. E., Kynaston, H., Gilbert, D. C., Wilson, R. H., Hubner, R. A., et al. (2023) · British Journal of Cancer · doi:10.1038/s41416-023-02310-1 · Cited by 34
Pangrazzi, L., & Meryk, A. (2024) · Biology · doi:10.3390/biology14010017 · Cited by 33
Garza, A. P., Morton, L., Pállinger, É., Buzás, E. I., Schreiber, S., Schott, B. H., & Dunay, I. R. (2023) · Brain Behavior & Immunity - Health · doi:10.1016/j.bbih.2023.100597 · Cited by 33
Śniadach, J., Kicman, A., Michalska‐Falkowska, A., Jończyk, K., & Waszkiewicz, N. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26051796 · Cited by 31
Bhattacharya, S. S., Yadav, B., Rosen, L., Nagpal, R., Yadav, H., & Yadav, J. S. (2022) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2022.116066 · Cited by 31
Zhang, Y., Archie, S. R., Ghanwatkar, Y., Sharma, S., Nozohouri, S., Burks, E., Mdzinarishvili, A., Liu, Z., & Abbruscato, T. J. (2022) · Fluids and Barriers of the CNS · doi:10.1186/s12987-022-00339-7 · Cited by 30
Arora, P., Athari, S. S., & Nainwal, L. M. (2022) · Food Bioscience · doi:10.1016/j.fbio.2022.101909 · Cited by 30
Yin, W., Lv, J., Yao, Y., Zhao, Y., He, Z., Wang, Q., Cui, L., & Dai, H. (2021) · Thoracic Cancer · doi:10.1111/1759-7714.14103 · Cited by 29
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Weskamp, P., Ufton, D., Drysch, M., Wagner, J. M., Dadras, M., Lehnhardt, M., Behr, B., & Wallner, C. (2022) · Cancers · doi:10.3390/cancers14051273 · Cited by 29
Gémes, N., Balog, J. Á., Neuperger, P., Schlegl, E., Barta, I., Fillinger, J., Antus, B., Zvara, Á., Hegedüs, Z., Czimmerer, Z., et al. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1297577 · Cited by 29
Long, E., Yin, J., Shin, J. H., Li, Y., Li, B., Kane, A., Patel, H., Sun, X., Wang, C., Luong, T., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-52356-9 · Cited by 28
Monocyte Single-Cell Multimodal Profiling in Cardiovascular Disease Risk States
Bashore, A. C., Xue, C., Kim, E., Yan, H., Zhu, L. Y., Pan, H., Kissner, M. D., Ross, L. S., Zhang, H., Li, M., et al. (2024) · Circulation Research · doi:10.1161/circresaha.124.324457 · Cited by 27
Self-citationZahedi, A. S., DANESHPOUR, M. S., Akbarzadeh, M., Hedayati, M., Azizi, F., & Zarkesh, M. (2023) · Heliyon · doi:10.1016/j.heliyon.2023.e19911 · Cited by 27
Catanzaro, G., Conte, F., Trocchianesi, S., Splendiani, E., Bimonte, V. M., Mocini, E., Filardi, T., Pò, A., Besharat, Z. M., Gentile, M. C., et al. (2023) · Scientific Reports · doi:10.1038/s41598-023-46516-y · Cited by 27
Zhu, Y., Webster, M. J., Walker, A. K., Massa, P. T., Middleton, F. A., & Weickert, C. S. (2023) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2023.03.018 · Cited by 26
Dolin, T. G., Christensen, I. J., Johansen, A. Z., Nielsen, H. J., Jakobsen, H. L., Klein, M. F., Lund, C. M., Bojesen, S. E., Nielsen, D. L., Jensen, B., et al. (2021) · Cancers · doi:10.3390/cancers14010161 · Cited by 24
White Blood Cell Count, Neutrophil-to-Lymphocyte Ratio, and Incident Cancer in the UK Biobank
Song, M., Graubard, B. I., Loftfield, E., Rabkin, C. S., & Engels, E. A. (2024) · Cancer Epidemiology Biomarkers & Prevention · doi:10.1158/1055-9965.epi-23-1145 · Cited by 24
Grievink, H. W., Smit, V., Huisman, B. W., Gal, P., Yavuz, Y., Klerks, C., Binder, C. J., Bot, I., Kuiper, J., Foks, A. C., et al. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.968815 · Cited by 23
Elisia, I., Yeung, M., Kowalski, S., Wong, J., Rafiei, H., Dyer, R., Atkar-Khattra, S., Lam, S. C., & Krystal, G. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.1051418 · Cited by 23
Self-citationKhan, M. A. B., BaHammam, A. S., Amanatullah, A., Obaideen, K., Arora, T., Ali, H. I., Ismail, L. C., Abdelrahim, D. N., Al‐Houqani, M., Allaham, K., et al. (2023) · Frontiers in Nutrition · doi:10.3389/fnut.2023.1040355 · Cited by 21
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Bian, D. J. H., Sabri, S., & Abdulkarim, B. (2022) · Cancers · doi:10.3390/cancers14153598 · Cited by 21
Kaastrup, K., & Grønbæk, K. (2021) · HemaSphere · doi:10.1097/hs9.0000000000000615 · Cited by 20
Fenercioğlu, A. K., Uzun, H., & Ünal, D. Ö. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms27010187 · Cited by 19
Bocharova, M., Borza, T., Watne, L. O., Engedal, K., O'Brien, J. T., Selbæk, G., Idland, A., Hodsoll, J. P., Young, A. H., & Aarsland, D. (2025) · Molecular Psychiatry · doi:10.1038/s41380-025-02908-2 · Cited by 19
Heikkinen, J., Tanner, T., Bergmann, U., Palosaari, S., & Lehenkari, P. P. (2024) · Tobacco Induced Diseases · doi:10.18332/tid/185281 · Cited by 18
Zhang, N., Liu, Y., Yang, C., Zeng, P., Gong, T., Tao, L., & Li, X.-A. (2022) · Tobacco Induced Diseases · doi:10.18332/tid/150340 · Cited by 18
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Sangani, R. G., Deepak, V., Anwar, J., Patel, Z., & Ghio, A. J. (2023) · International Journal of COPD · doi:10.2147/copd.s397667 · Cited by 17
Sarıkaya, B., Kolay, E., Guney-Coskun, M., Yiğit, A., & Aktaç, Ş. (2024) · Nutrition Reviews · doi:10.1093/nutrit/nuae143 · Cited by 17
Hajilo, P., Imani, B., Zandi, S., Mehrafshan, A., & Khazaei, S. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e40607 · Cited by 17
Pivneva, I., Balp, M., Geissbühler, Y., Severin, T. M., Smeets, S. J., Signorovitch, J., Royer, J., Liang, Y., Cornwall, T., Pan, J., et al. (2022) · Dermatology and Therapy · doi:10.1007/s13555-022-00827-6 · Cited by 17
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Iglesias‐Vázquez, L., Arija, V., Aranda, N., Aglago, E. K., Cross, A. J., Schulze, M. B., Pacheco, D. A. Q., Kuhn, T. E., Weiderpass, E., Tumino, R., et al. (2021) · European Journal of Nutrition · doi:10.1007/s00394-021-02625-w · Cited by 16
Aishwarya, S., Siddalingaswamy, P. C., & Chadaga, K. (2025) · Scientific Reports · doi:10.1038/s41598-025-09409-w · Cited by 15
Frazier, J. A., Li, X., Kong, X., Hooper, S. R., Joseph, R. M., Cochran, D., Kim, S., Fry, R. C., Brennan, P. A., Msall, M. E., et al. (2023) · Journal of the American Academy of Child & Adolescent Psychiatry · doi:10.1016/j.jaac.2023.05.010 · Cited by 15
Kendirlioglu, B. K., Arat-Çelik, H. E., Tunç, A. E. B., Özmen, M., Kaymakçı, E. Ç., DEMİR, Ş., & Küçükgöncü, S. (2023) · Journal of Immunoassay and Immunochemistry · doi:10.1080/15321819.2023.2277806 · Cited by 15
Świątkowska, B., Jankowski, M., & Kaleta, D. (2024) · Scientific Reports · doi:10.1038/s41598-024-59321-y · Cited by 15
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Yue, Z., Fan, Y., Shan, G., & Chen, X. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1630828 · Cited by 15
Wang, S., Li, Q., Wei, H., Jiang, F., & Yan, A. (2022) · World Allergy Organization Journal · doi:10.1016/j.waojou.2022.100630 · Cited by 15
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Skalny, A. V., Serebryansky, E. P., Korobeinikova, T. V., Tsatsakis, A. M., Vardavas, C. I., Paoliello, M. M. B., Sotnikova, T. I., Aschner, M., & Tinkov, A. A. (2022) · Food and Chemical Toxicology · doi:10.1016/j.fct.2022.113249 · Cited by 15
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Palma, M. I. V., Klivinyi, C., Lampl, T. S., Lang-Illievich, K., Bornemann‐Cimenti, H., & Szilagyi, I.-S. (2022) · Pain and Therapy · doi:10.1007/s40122-022-00462-1 · Cited by 15
Zhu, Y., Webster, M. J., Victoriano, G. M., Middleton, F. A., Massa, P. T., & Weickert, C. S. (2024) · Schizophrenia Bulletin · doi:10.1093/schbul/sbae184 · Cited by 14
Patel, S. K., Tran, S. D., Overbey, J., Ksajikian, A., Gupta, R., Kenter, K., Bovid, K. M., & Li, Y. (2025) · The Journal of Arthroplasty · doi:10.1016/j.arth.2025.03.064 · Cited by 13
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Arima, H. (2023) · BMC Public Health · doi:10.1186/s12889-023-17418-5 · Cited by 11
Lee, E. H., Lee, K. H., Lee, K., Park, Y., Han, K. D., & Han, S. H. (2024) · Journal of Epidemiology and Global Health · doi:10.1007/s44197-024-00197-6 · Cited by 11
Rinderknecht, H., Nussler, A. K., Steinestel, K., Histing, T., & Ehnert, S. (2022) · Bioengineering · doi:10.3390/bioengineering9050186 · Cited by 11
Iwasaki, M., Ishihara, S., Okada, S., Shimegi, R., Shimomura, M., & Inoue, M. (2022) · Annals of Surgical Oncology · doi:10.1245/s10434-022-11835-3 · Cited by 11
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Tsuo, K., Argentieri, M. A., Gadd, D. A., Kurki, M., Zheng, Z., Baird, D. A., Marioni, R. E., Foley, C. N., Huang, H., Sun, B. B., et al. (2025) · medRxiv · doi:10.1101/2025.08.27.25334571 · Cited by 8
Dahal, S., Yingst, J. M., Wang, X., Cobb, C. O., Carrillo, M., Hrabovsky, S., Bascom, R., Lopez, A. A., Kang, L., Maloney, S. F., et al. (2025) · BMJ Open · doi:10.1136/bmjopen-2024-098005 · Cited by 7
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Soenen, R., Schots, L., Wang, Z., Tilleman, L., Nieuwerburgh, F. V., Grine, L., Temmerman, L., Hillary, T., Stockman, A., Dreesen, E., et al. (2024) · Journal of the European Academy of Dermatology and Venereology · doi:10.1111/jdv.20074 · Cited by 7
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Mao, J., Wang, Y., Chen, W., Wang, X., Liu, J., Shao, Y., & Tu, Z. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.134642 · Cited by 7
Nowak, J. K., Dybska, E., Adams, A. T., & Walkowiak, J. (2022) · Central European Journal of Immunology · doi:10.5114/ceji.2022.120618 · Cited by 7
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Sawvell, E., Martinez, A. V., Walker, L., Wu, Y. R., Burnikel, B. G., & Mercuri, J. J. (2024) · Journal of Orthopaedic Research® · doi:10.1002/jor.25828 · Cited by 6
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Mahmoud, A. A., Abdel-Aziz, H. O., Elbadr, M. M., & ElBadre, H. M. (2021) · Reports of Biochemistry and Molecular Biology · doi:10.52547/rbmb.10.3.429 · Cited by 6
Sansa, A., Valero, C., Pujol, A., Sauter, B., Gayà, J., Quer, M., & León, X. (2024) · Acta Otorrinolaringologica (English Edition) · doi:10.1016/j.otoeng.2023.07.003 · Cited by 5
Jalalvand, M., Tahermohammadi, H., Madreseh, E., Varahram, M., Sharifi, H., Akhtari, M., & Jamaati, H. (2025) · Respiratory Medicine · doi:10.1016/j.rmed.2025.108104 · Cited by 5
Brustad, N., Wang, T., Chen, L., Kaiser, H., Gomes, B., Klein, A., Vahman, N., Skov, L., Stokholm, J., Schoos, A. M., et al. (2025) · Journal of the American Academy of Dermatology · doi:10.1016/j.jaad.2025.08.044 · Cited by 5
Li, G., He, L., Bai, X., Chen, S., Dai, H., He, G., Yang, Y., Zhang, X., Cui, J., Li, X., et al. (2025) · The Lancet Regional Health - Western Pacific · doi:10.1016/j.lanwpc.2025.101689 · Cited by 5
Framingham risk score associates with incident cancer and heart failure
van den Berg, P. F., Yousif, L. I. E., Koop, Y., Ünlü, E. H., Asik, M., van Essen, B. J., Damman, K., Voors, A. A., Sayour, N. V., Kok, T. F., et al. (2025) · European Journal of Preventive Cardiology · doi:10.1093/eurjpc/zwaf618 · Cited by 5
Demirel, F., & Arıkan, İ. İ. (2025) · African Health Sciences · doi:10.4314/ahs.v25i3.11 · Cited by 5
Nicholas, J. C., Alkis, T., Bis, J. C., Boerwinkle, E., Brody, J. A., Clish, C. B., de Vries, P. S., Gao, Y., Gerszten, R. E., Guo, X., et al. (2026) · Journal of Thrombosis and Haemostasis · doi:10.1016/j.jtha.2025.12.016 · Cited by 5
Yan, J., Wang, J., Jian, F., Cui, X., Zhang, Y., Yang, X., Gao, Q., Ding, Z., 汤钊猷, Fan, J., et al. (2026) · Portal hypertension & cirrhosis · doi:10.1002/poh2.70033 · Cited by 5
Josefsson, D., Danielsson, L., & Jakobsson, M. (2026) · Frontiers in Pain Research · doi:10.3389/fpain.2026.1755744 · Cited by 5
Metadata
- Authors
- Elisia, I., Lam, V., Cho, B., Hay, M., Li, M. Y., Yeung, M., Bu, L., Jia, W., Norton, N., Lam, S., & Krystal, G.
- Affiliations
- University of British Columbia (CA)
- Corresponding author
- Krystal, Gerald
- Venue
- Scientific Reports
- Published
- 2020-11-10
- Volume
- 10
- Issue
- 1
- Pages
- 19480-19480
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC7655856
- OpenAlex
- W3098547973
- Semantic Scholar
- 2d338a0e15102ec7f2f5e47d29abf9e9e167b97e
- Cited by
- 263
- References
- 77
- Citations by year
- 2026: 62 · 2025: 68 · 2024: 59 · 2023: 37 · 2022: 28 · 2021: 9
- FWCI
- 13.54
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- Funders
- Lotte and John Hecht Memorial Foundation (3693)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Smoking Behavior and Cessation
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Keywords
- smoking, chronic inflammation, immune function, blood cell composition, C-reactive protein, interleukin-6, fibrinogen, cytokines and chemokines, Escherichia coli challenge, prostaglandin E2, natural killer cells, regulatory T cells, granulocytes, monocytes, red blood cell indices, lung cancer risk, heavy smokers
- MeSH terms
- Aged, Blood Cell Count, C-Reactive Protein, Carcinoembryonic Antigen, Chronic Disease, Female, Fibrinogen, Humans, Immunity, Innate, Inflammation, Male, Middle Aged, Smoking, Biomarkers, Interleukin-6, Cytokines, Adaptive Immunity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
A language model drew the claims from the paper on 2026-11-13, under version 3 of the claim definition (model deepseek-v4.1-flash, prompt extract-v3).
A second model labelled each claim on 2026-11-13 (model jev-1.13-20260917, definition revision gold-guideline-v0.2).
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
