Human skin explant model for the investigation of topical therapeutics
Open accessAbstract
The development of in vitro and ex vivo models to mimic human illness is important not only for scientific understanding and investigating therapeutic approaches but also to mitigate animal testing and bridge the inter-species translational gap. While in vitro models can facilitate high-throughput and cost-efficient evaluation of novel therapeutics, more complex ex vivo systems can better predict both desirable and adverse in vivo effects. Here we describe an ex vivo cultured human skin explant model in which we have characterized pathological tissue integrity, barrier function and metabolic stability over time. Our findings suggest that human skin can be successfully cultured for pharmacodynamic use up to and beyond 9 days without any adverse physiological consequence.
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Metabolism is far less in previously frozen skin kept on saline solution for 24 hours compared to in vivo.
“Metabolism is known to be far less in previously frozen skin kept on saline solution for 24 hours compared to in vivo (Neil et al., 2020).”
From Human skin absorption of three phthalates (2024) · cites this paper as Neil et al., 2020
The Conclusions (page unavailable) report a discrepancy in metabolic biomarkers between the cultured human skin explant model and skin in vivo. They do not say metabolism is far less in previously frozen skin, or assess skin kept in saline for 24 hours. The Methods describe tissue kept chilled before culture in modified Cornification medium, not previously frozen tissue held in saline. (LLM, conf=0.78)
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- Authors
- Neil, J. E., Brown, M. B., & Williams, A. C.
- Affiliations
- MedPharm (United Kingdom) (GB)
- University of Reading (GB)
- Corresponding author
- Neil, Jessica E.
- Venue
- Scientific Reports
- Published
- 2020-12-03
- Volume
- 10
- Issue
- 1
- Pages
- 21192-21192
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC7712775
- OpenAlex
- W3110339053
- Cited by
- 90
- References
- 32
- Citations by year
- 2026: 14 · 2025: 19 · 2024: 25 · 2023: 13 · 2022: 15 · 2021: 4
- FWCI
- 3.11
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- CentAUR (University of Reading) (submittedVersion)
- Funders
- MedPharm
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Health Sciences › Veterinary › Small Animals › Animal testing and alternatives
- Keywords
- human skin explants, ex vivo skin model, skin barrier function, skin explant culture, metabolic stability, tissue integrity, alternatives to animal testing, translational research, pharmacodynamics
- MeSH terms
- Administration, Topical, Humans, Models, Biological, Organ Culture Techniques, Proteins, Skin, Skin Absorption, Keratinocytes, In Vitro Techniques
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