The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Nova autoregulation reveals dual functions in neuronal splicing
Open accessAbstract
The Nova family of neuron-specific RNA-binding proteins were originally identified as targets in an autoimmune neurologic disease characterized by failure of motor inhibition. Nova-1 regulates alternative splicing of pre-mRNAs encoding the inhibitory neurotransmitter receptor subunits GABA(A)Rgamma2 and GlyRalpha2 by directly binding intronic elements, resulting in enhancement of exon inclusion. Here we identify exon E4 in the Nova-1 pre-mRNA itself, encoding a phosphorylated protein domain, as an additional target of Nova-dependent splicing regulation in the mouse spinal cord. Nova binding to E4 is necessary and sufficient for Nova-dependent exon exclusion. E4 harbors five repeats of the known Nova-binding tetranucleotide YCAY and mutation of these elements destroys Nova-dependent regulation. Furthermore, swapping of the sites from Nova-1 and GABA(A)Rgamma2 indicates that the ability of Nova to enhance or repress alternative exon inclusion is dependent on the position of the Nova-binding element within the pre-mRNA. These studies demonstrate that in addition to its previously described role as a splicing activator, Nova autoregulates its own expression by acting as a splicing repressor.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“The mutually exclusive expression pattern of SLM1 and SLM2 is enforced by their cross-regulation via alternative splicing coupled with NMD, as seen with other paralogous pairs of RNA-binding proteins15,20,21,49,53,85,86.”
From The neurogenetics of alternative splicing (2016) · cites this paper as [49]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Mechanisms of Alternative Pre-Messenger RNA Splicing
Black, D. L. (2003) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.72.121801.161720 · Cited by 2,704
Listening to silence and understanding nonsense: exonic mutations that affect splicing
Cartegni, L., Chew, S. L., & Krainer, A. R. (2002) · Nature Reviews Genetics · doi:10.1038/nrg775 · Cited by 2,209
PEST sequences and regulation by proteolysis
Rechsteiner, M., & Rogers, S. W. (1996) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(96)10031-1 · Cited by 1,707
ESEfinder: a web resource to identify exonic splicing enhancers
Cartegni, L. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg616 · Cited by 1,593
Genome-Wide Survey of Human Alternative Pre-mRNA Splicing with Exon Junction Microarrays
Johnson, J. M., Castle, J. C., Garrett-Engele, P. W., Kan, Z., Loerch, P., Armour, C. D., Santos, R., Schadt, E. E., Stoughton, R. B., & Shoemaker, D. D. (2003) · Science · doi:10.1126/science.1090100 · Cited by 1,418
CLIP Identifies Nova-Regulated RNA Networks in the Brain
Ule, J., Jensen, K. B., Ruggiu, M., Mele, A., Ule, A., & Darnell, R. B. (2003) · Science · doi:10.1126/science.1090095 · Cited by 1,120
Self-citationParaneoplastic Syndromes Involving the Nervous System
Darnell, R. B., & Posner, J. B. (2003) · New England Journal of Medicine · doi:10.1056/nejmra023009 · Cited by 1,067
Self-citationPredictive Identification of Exonic Splicing Enhancers in Human Genes
Fairbrother, W. G., Yeh, R., Sharp, P. A., & Burge, C. B. (2002) · Science · doi:10.1126/science.1073774 · Cited by 1,020
Protein kinase recognition sequence motifs
Kemp, B. E., & Pearson, R. B. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90073-k · Cited by 988
Cited by
Sorted by citations
Items 101–125 of 125
Michielsen, L., Hsu, J., Joglekar, A., Belchikov, N., Reinders, M. J. T., Tilgner, H., & Mahfouz, A. E. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.03.18.585465 · Cited by 4
Paraneoplastic Neurological Syndromes
Spatola, M. (2021) · Springer eBooks · doi:10.1007/978-3-030-61883-4_16 · Cited by 3
Impact of Acute Endurance Exercise on Alternative Splicing in Skeletal Muscle
Ahn, A., Kim, J. J., Slusher, A. L., Ying, J. Y., Zhang, E. Y., & Ludlow, A. T. (2025) · FASEB BioAdvances · doi:10.1096/fba.2025-00007 · Cited by 4
Titus, M. B., Chang, A. W., Popitsch, N. P., Ebmeier, C. C., Bono, J. M., & Olesnicky, E. C. (2023) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2023.1114857 · Cited by 2
Evsyukova, I. Y. (2012) · Cited by 2
Song, Z., Jiang, J., Tang, T., Luo, G., Wang, J., Lin, K., Mu, L., & Zhao, C. (2025) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2025.106556 · Cited by 1
Hossain, M. F., Popsuj, S., Vitrinel, B., Kaplan, N., Stolfi, A., Christiaen, L., & Ruggiu, M. (2025) · PLoS Biology · doi:10.1371/journal.pbio.3003392 · Cited by 1
Dembowski, J. A. (2009) · D-Scholarship@Pitt (University of Pittsburgh) · Cited by 1
Multidimensional control of cell structural robustness
Grinev, V. V., Ramanouskaya, T. V., & Gloushen, S. V. (2013) · Cell Biology International · doi:10.1002/cbin.10128 · Cited by 2
RNA regulation in Neurodegeneration and Cancer
Darnell, R. B. (2010) · Research and perspectives in Alzheimer's disease · doi:10.1007/978-3-642-16602-0_9 · Cited by 1
Self-citationEvolution of Nova-dependent splicing regulation in the brain
Jelen, N., Ule, J., Živin, M., & Darnell, R. B. (2005) · PLoS Genetics · doi:10.1371/journal.pgen.0030173.eor · Cited by 1
Self-citationSplicing Decisions Shape Neuronal Protein Function across the Transcriptome
Dembowski, J. A., & Grabowski, P. J. (2013) · Wiley eBooks · doi:10.1002/9783527665433.ch7
The CLIP Method to Study Protein–RNA Interactions in Intact Cells and Tissues
Tollervey, J. R., & Ule, J. (2012) · Wiley eBooks · doi:10.1002/9783527636778.ch25
Recent Advances in Understanding of Alternative Splicing in Neuronal Pathogenesis
Chen, H.-H., Lin, J.-C., & Tar, W.-Y. (2011) · InTech eBooks · doi:10.5772/22477
Yeo, G. W.-M., Nostrand, E. L. V., & Liang, T. Y. (2005) · PLoS Genetics · doi:10.1371/journal.pgen.0030085.eor
Identification and characterization of murine Se70-2
Minnema, S. E. (2006) · Libraries and Cultural Resources (University of Calgary) · doi:10.11575/prism/10915
Regulation of Core Splicing Factors by Alternative Splicing and Nonsense-mediated mRNA Decay
Saltzman, A. L. (2012) · TSpace (University of Toronto)
Paraneoplastic Neurologic Syndromes
Dalmau, J. O. (2007) · Elsevier eBooks · doi:10.1016/b978-012369509-3.50034-2
Stationary Analysis the Alternative Splicing Profile Reveals the Splicing CodePreprint
Li, M. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/129866
Yotova, A. Y. (2024) · Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) · doi:10.21248/gups.85791
A conserved RNA switch for acetylcholine receptor clustering at neuromuscular junctions in chordatesPreprint
Hossain, M. F., Popsuj, S., Vitrinel, B., Kaplan, N., Stolfi, A., Christiaen, L., & Ruggiu, M. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.07.05.602308
Michielsen, L., Hsu, J., Joglekar, A., Belchikov, N., Reinders, M. J. T., Tilgner, H., & Mahfouz, A. E. (2026) · Genome biology · doi:10.1186/s13059-026-04015-z · Cited by 3
Metadata
- Authors
- Dredge, B. K., Stefani, G., Engelhard, C. C., & Darnell, R. B.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Darnell, Robert B
- Venue
- The EMBO Journal
- Published
- 2005-03-31
- Volume
- 24
- Issue
- 8
- Pages
- 1608-1620
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC1142566
- OpenAlex
- W2150107170
- Cited by
- 125
- References
- 30
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 6 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 5 · 2018: 3 · 2017: 4 · 2016: 6 · 2014: 4 · 2013: 8 · 2012: 11
- FWCI
- 4.41
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
- Iris (University of Trento) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institute of Neurological Disorders and Stroke (R01NS040955, R01 NS34389, R01NS034389, NS40955)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- alternative splicing, autoregulation, RNA-binding proteins, exon inclusion, spinal cord neurons, splicing repressor, phosphorylation domain
- MeSH terms
- Neuro-Oncological Ventral Antigen, Amino Acid Sequence, Animals, Antigens, Neoplasm, Base Sequence, Binding Sites, Cell Line, Exons, Gene Expression Regulation, Humans, Molecular Sequence Data, Nerve Tissue Proteins, Neurons, Receptors, GABA-A, RNA Precursors, Sequence Alignment, RNA-Binding Proteins, Alternative Splicing, Protein Structure, Tertiary, Receptors, Glycine, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
