Nova-1 Regulates Neuron-Specific Alternative Splicing and Is Essential for Neuronal Viability
Open accessAbstract
We have combined genetic and biochemical approaches to analyze the function of the RNA-binding protein Nova-1, the paraneoplastic opsoclonus-myoclonus ataxia (POMA) antigen. Nova-1 null mice die postnatally from a motor deficit associated with apoptotic death of spinal and brainstem neurons. Nova-1 null mice show specific splicing defects in two inhibitory receptor pre-mRNAs, glycine alpha2 exon 3A (GlyRalpha2 E3A) and GABA(A) exon gamma2L. Nova protein in brain extracts specifically bound to a previously identified GlyRalpha2 intronic (UCAUY)3 Nova target sequence, and Nova-1 acted directly on this element to increase E3A splicing in cotransfection assays. We conclude that Nova-1 binds RNA in a sequence-specific manner to regulate neuronal pre-mRNA alternative splicing; the defect in splicing in Nova-1 null mice provides a model for understanding the motor dysfunction in POMA.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
NOVA1 is an interactor of ELAVL4, a gene that codes for a neuronal-specific RNA-binding protein and might have been under positive selection in humans.
“NOVA1101, which is an interactor of ELAVL4102, a gene that codes for a neuronal-specific RNA-binding protein and might have been under positive selection in humans33,36.”
From A catalog of single nucleotide changes distinguishing modern humans from archaic hominins (2019) · cites this paper as [101]
Nova1/2 dKO mice, but not single-knockout mice, show fewer acetylcholine receptor clusters and a loss of apposition between AChR clusters and phrenic nerve terminals.
“the dKO mice, but not the single-knockout mice, show fewer acetylcholine receptor (AChR) clusters and a loss of apposition between AChR clusters and phrenic nerve terminals”
From The neurogenetics of alternative splicing (2016) · cites this paper as [38]
Nova1−/− mice appear normal at birth but die in the second postnatal week with motor neuron apoptosis, profound motor failure and action-induced tremors.
“Nova1−/− mice appear normal at birth, but die in the second postnatal week with motor neuron apoptosis, profound motor failure and action-induced tremors37,38.”
From The neurogenetics of alternative splicing (2016) · cites this paper as [38]
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,240 · see citing works
Hollmann, M., & Heinemann, S. F. (1994) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.17.030194.000335 · Cited by 4,011 · see citing works
PHARMACOLOGY AND FUNCTIONS OF METABOTROPIC GLUTAMATE RECEPTORS
Conn, P. J., & Pin, J. (1997) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.37.1.205 · Cited by 3,083 · see citing works
Amara, S. G., Jonas, V., Rosenfeld, M. G., Ong, E. S., & Evans, R. M. (1982) · Nature · doi:10.1038/298240a0 · Cited by 2,105 · see citing works
Visualizing an Olfactory Sensory Map
Mombaerts, P., Wang, F., Dulac, C. G., Chao, S. K., Nemes, A., Mendelsohn, M. M., Edmondson, J., & Axel, R. (1996) · Cell · doi:10.1016/s0092-8674(00)81387-2 · Cited by 1,992 · see citing works
Conserved Structures and Diversity of Functions of RNA-Binding Proteins
Burd, C. G., & Dreyfuss, G. (1994) · Science · doi:10.1126/science.8036511 · Cited by 1,878 · see citing works
Flip and Flop: A Cell-Specific Functional Switch in Glutamate-Operated Channels of the CNS
Sommer, B., KEINÄNEN, K. P., Verdoorn, T. A., Wisden, W., Burnashev, N., Herb, A., Köhler, M. G., Takagi, T., Sakmann, B. J., & Seeburg, P. H. (1990) · Science · doi:10.1126/science.1699275 · Cited by 1,259 · see citing works
Wang, L., Miura, M., Bergeron, L., Zhu, H., & Yuan, J. (1994) · Cell · doi:10.1016/s0092-8674(94)90422-7 · Cited by 836 · see citing works
Absence Epilepsy in Tottering Mutant Mice Is Associated with Calcium Channel Defects
Fletcher, C., Lutz, C. M., O’Sullivan, T. N., Shaughnessy, J. D., Hawkes, R. B., Frankel, W. N., Copeland, N. G., & Jenkins, N. A. (1996) · Cell · doi:10.1016/s0092-8674(00)81381-1 · Cited by 772 · see citing works
FMR1 Protein: Conserved RNP Family Domains and Selective RNA Binding
Ashley, C. T., Wilkinson, K. D., Reines, D., & Warren, S. T. (1993) · Science · doi:10.1126/science.7692601 · Cited by 741 · see citing works
A Striking Organization of a Large Family of Human Neural Cadherin-like Cell Adhesion Genes
Wu, Q., & Maniatis, T. (1999) · Cell · doi:10.1016/s0092-8674(00)80789-8 · Cited by 714 · see citing works
A critical role for DNA end-joining proteins in both lymphogenesis and neurogenesis
Gao, Y., Sun, Y., Frank, K. M., Dikkes, P., Fujiwara, Y., Seidl, K. J., Sekiguchi, J. M., Rathbun, G. A., Swat, W., Wang, J., et al. (1998) · Cell · doi:10.1016/s0092-8674(00)81714-6 · Cited by 688 · see citing works
Lin, C.-L., Bristol, L. A., Jin, L., Dykes‐Hoberg, M., Crawford, T. O., Clawson, L., & Rothstein, J. D. (1998) · Neuron · doi:10.1016/s0896-6273(00)80997-6 · Cited by 672 · see citing works
The SMN–SIP1 Complex Has an Essential Role in Spliceosomal snRNP Biogenesis
Fischer, U., Liu, Q., & Dreyfuss, G. (1997) · Cell · doi:10.1016/s0092-8674(00)80368-2 · Cited by 657 · see citing works
The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein.
Siomi, H., Siomi, M. C., Nussbaum, R. L., & Dreyfuss, G. (1993) · Cell · doi:10.1016/0092-8674(93)90420-u · Cited by 641 · see citing works
Regulation of Alternative Splicing in Vivo by Overexpression of Antagonistic Splicing Factors
Cáceres, J. F., Stamm, S., Helfman, D. M., & Krainer, A. R. (1994) · Science · doi:10.1126/science.8085156 · Cited by 619 · see citing works
Zinc potentiates agonist-lnduced currents at certain splice variants of the NMDA receptor
Hollmann, M., Boulter, J., Maron, C., Beasley, L., Sullivan, J., Pecht, G., & Heinemann, S. F. (1993) · Neuron · doi:10.1016/0896-6273(93)90209-a · Cited by 611 · see citing works
Szabo, A., Dalmau, J., Manley, G., Rosenfeld, M., Wong, E., Henson, J., Posner, J. B., & Furneaux, H. M. (1991) · Cell · doi:10.1016/0092-8674(91)90184-z · Cited by 553 · see citing works
Mouse-Musashi-1, a Neural RNA-Binding Protein Highly Enriched in the Mammalian CNS Stem Cell
Sakakibara, S., Imai, T., Hamaguchi, K., Okabe, M., Aruga, J., Nakajima, K., Yasutomi, D., Nagata, T., Kurihara, Y., Uesugi, S., et al. (1996) · Developmental Biology · doi:10.1006/dbio.1996.0130 · Cited by 526 · see citing works
Self-citationOstareck, D. H., Ostareck‐Lederer, A., Wilm, M. S., Thiele, B. J., Mann, M., & Hentze, M. W. (1997) · Cell · doi:10.1016/s0092-8674(00)80241-x · Cited by 503 · see citing works
Shiang, R., Ryan, S., Zhu, Y.-Z., Hahn, A., O'Connell, P., & Wasmuth, J. (1993) · Nature Genetics · doi:10.1038/ng1293-351 · Cited by 502 · see citing works
Sex in flies: the splice of life
Baker, B. S. (1989) · Nature · doi:10.1038/340521a0 · Cited by 468 · see citing works
Ullrich, B., Ushkaryov, Y. A., & Südhof, T. C. (1995) · Neuron · doi:10.1016/0896-6273(95)90306-2 · Cited by 460 · see citing works
Tumor-specific killer cells in paraneoplastic cerebellar degeneration
Albert, M. L., Darnell, J. C., Bender, A., Francisco, L. M., Bhardwaj, N., & Darnell, R. B. (1998) · Nature Medicine · doi:10.1038/3315 · Cited by 449 · see citing works
Self-citationDiversity Revealed by a Novel Family of Cadherins Expressed in Neurons at a Synaptic Complex
Kohmura, N., Senzaki, K., Hamada, S., Kai, N., Yasuda, R., Watanabe, M., Ishii, H., Yasuda, M., Mishina, M., & Yagi, T. (1998) · Neuron · doi:10.1016/s0896-6273(00)80495-x · Cited by 444 · see citing works
Anti‐Ri: An antibody associated with paraneoplastic opsoclonus and breast cancer
Luque, F. A., Furneaux, H. M., Ferziger, R., et al. (1991) · Annals of Neurology · doi:10.1002/ana.410290303 · Cited by 403 · see citing works
A Hierarchy of Hu RNA Binding Proteins in Developing and Adult Neurons
Okano, H. J., & Darnell, R. B. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-09-03024.1997 · Cited by 379 · see citing works
Self-citationMichael, W. M., Eder, P. S., & Dreyfuss, G. (1997) · The EMBO Journal · doi:10.1093/emboj/16.12.3587 · Cited by 378 · see citing works
Neurexins: three genes and 1001 products.
Missler, M., & Südhof, T. (1998) · Trends in Genetics · doi:10.1016/s0168-9525(97)01324-3 · Cited by 376 · see citing works
A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer.Preprint
Min, H., Turck, C. W., Nikolic, J. M., & Black, D. L. (1997) · Genes & Development · doi:10.1101/gad.11.8.1023 · Cited by 330 · see citing works
Sequence-Specific RNA Binding by a Nova KH Domain
Lewis, H. A., Musunuru, K., Jensen, K. B., Edo, C., Chen, H., Darnell, R. B., & Burley, S. K. (2000) · Cell · doi:10.1016/s0092-8674(00)80668-6 · Cited by 319 · see citing works
Self-citationBuckanovich, R. J., Posner, J. B., & Darnell, R. B. (1993) · Neuron · doi:10.1016/0896-6273(93)90077-5 · Cited by 318 · see citing works
Self-citationValcárcel, J., Singh, R., Zamore, P. D., & Green, M. R. (1993) · Nature · doi:10.1038/362171a0 · Cited by 309 · see citing works
Neuronal pp60 c- src Contains a Six-Amino Acid Insertion Relative to Its Non-Neuronal Counterpart
Martinez, R., Mathey-Prévôt, B., Bernards, A., & Baltimore, D. (1987) · Science · doi:10.1126/science.2440106 · Cited by 305 · see citing works
Regulated Subcellular Distribution of the NR1 Subunit of the NMDA Receptor
Ehlers, M., Tingley, W. G., & Huganir, R. L. (1995) · Science · doi:10.1126/science.7569904 · Cited by 293 · see citing works
Rajendra, S., Lynch, J. W., & Schofield, P. R. (1997) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(96)00163-5 · Cited by 288 · see citing works
Darnell, R. B. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.10.4529 · Cited by 276 · see citing works
Self-citationEhlers, M., Fung, E. T., O’Brien, R., & Huganir, R. L. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-02-00720.1998 · Cited by 267 · see citing works
The Neuronal RNA Binding Protein Nova-1 Recognizes Specific RNA Targets In Vitro and In Vivo
Buckanovich, R. J., & Darnell, R. B. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.6.3194 · Cited by 259 · see citing works
Self-citationMechanisms for selecting 5′ splice sites in mammalian pre-mRNA splicing
Horowitz, D. S., & Krainer, A. R. (1994) · Trends in Genetics · doi:10.1016/0168-9525(94)90233-x · Cited by 257 · see citing works
Development of Glycinergic Synaptic Transmission to Rat Brain Stem Motoneurons
Singer, J. H., Talley, E. M., Bayliss, D. A., & Berger, A. J. (1998) · Journal of Neurophysiology · doi:10.1152/jn.1998.80.5.2608 · Cited by 256 · see citing works
Chou, M., Rooke, N., Turck, C. W., & Black, D. L. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.1.69 · Cited by 251 · see citing works
Sequential expression and role of Hu RNA-binding proteins during neurogenesis
Wakamatsu, Y., & Weston, J. A. (1997) · Development · doi:10.1242/dev.124.17.3449 · Cited by 240 · see citing works
Yang, Y. Y. L., Yin, G. L., & Darnell, R. B. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.22.13254 · Cited by 219 · see citing works
Self-citationQuantitation of RNA using the polymerase chain reaction
Foley, K. P., Leonard, M. W., & Engel, J. D. (1993) · Trends in Genetics · doi:10.1016/0168-9525(93)90137-7 · Cited by 217 · see citing works
Chan, R. C., & Black, D. L. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.8.4667 · Cited by 216 · see citing works
Ferns, M., Hoch, W., Campanelli, J. T., Rupp, F., Hall, Z. W., & Scheller, R. H. (1992) · Neuron · doi:10.1016/0896-6273(92)90129-2 · Cited by 212 · see citing works
Bockaert, J., Claeysen, S., Compan, V., & Dumuis, A. (2004) · CNS & Neurological Disorders - Drug Targets · doi:10.2174/1568007043482615 · Cited by 207 · see citing works
Positive Control of Pre-mRNA Splicing in Vitro
Tian, M., & Maniatis, T. (1992) · Science · doi:10.1126/science.1566072 · Cited by 200 · see citing works
Buckanovich, R., Yang, Y., & Darnell, R. (1996) · The Journal of Neuroscience · doi:10.1523/jneurosci.16-03-01114.1996 · Cited by 192 · see citing works
Self-citationRyan, S. G., Buckwalter, M. S., Lynch, J. W., Handford, C. A., Segura, L., Shiang, R., Wasmuth, J. J., Camper, S. A., Schofield, P. R., & O'Connell, P. (1994) · Nature Genetics · doi:10.1038/ng0694-131 · Cited by 186 · see citing works
Activation of c-src neuron-specific splicing by an unusual RNA element in vivo and in vitro
Black, D. L. (1992) · Cell · doi:10.1016/0092-8674(92)90291-j · Cited by 172 · see citing works
Petersen‐Mahrt, S. K., Nilsson, C. E., Öhrmalm, C., Matthews, D. A., Russell, W. C., & Akusjärvi, G. (1999) · The EMBO Journal · doi:10.1093/emboj/18.4.1014 · Cited by 171 · see citing works
Cytoplasmic regulatory functions of the KH-domain proteins hnRNPs K and E1/E2
Ostareck‐Lederer, A., Ostareck, D. H., & Hentze, M. W. (1998) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(98)01301-2 · Cited by 164 · see citing works
Opioid Receptor Types and Subtypes: The delta Receptor as a Model
Zaki, P. A., Bilsky, E. J., Vanderah, T. W., et al. (1996) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.36.040196.002115 · Cited by 164 · see citing works
Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA.
Arning, S., Grüter, P., Bilbe, G., et al. (1996) · PubMed · Cited by 154 · see citing works
Lou, H. J., Helfman, D. M., Gagel, R. F., & Berget, S. M. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.1.78 · Cited by 149 · see citing works
Alternative splicing generates two isoforms of the α2 subunit of the inhibitory glycine receptor
Kuhse, J., Kuryatov, A., Maulet, Y., et al. (1991) · FEBS Letters · doi:10.1016/0014-5793(91)80557-j · Cited by 144 · see citing works
Common themes in the function of transcription and splicing enhancers
Hertel, K. J., Lynch, K. W., & Maniatis, T. (1997) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(97)80007-5 · Cited by 143 · see citing works
Splicing Regulation in Neurons: Tinkering with Cell-Specific Control
Grabowski, P. J. (1998) · Cell · doi:10.1016/s0092-8674(00)81399-9 · Cited by 126 · see citing works
Dopamine D2 Receptors in Signal Transduction and Behavior
Picetti, R., Saiardi, A., Samad, T. A., Bozzi, Y., Baik, J., & Borrelli, E. (1997) · Critical Reviews in Neurobiology · doi:10.1615/critrevneurobiol.v11.i2-3.20 · Cited by 125 · see citing works
MER1 , a Yeast Gene Required for Chromosome Pairing and Genetic Recombination, Is Induced in Meiosis
Engebrecht, J., & Roeder, G. S. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.5.2379-2389.1990 · Cited by 124 · see citing works
Soma-specific expression and cloning of PSI, a negative regulator of P element pre-mRNA splicing.Preprint
Siebel, C. W., Admon, A., & Rio, D. C. (1995) · Genes & Development · doi:10.1101/gad.9.3.269 · Cited by 120 · see citing works
Okano, H. J., Park, W.-Y., Corradi, J., & Darnell, R. B. (1999) · Genes & Development · doi:10.1101/gad.13.16.2087 · Cited by 116 · see citing works
Self-citationCoordinate repression of a trio of neuron-specific splicing events by the splicing regulator PTB
ZHANG, L., Liu, W., & Grabowski, P. J. (1999) · RNA · doi:10.1017/s1355838299981530 · Cited by 115 · see citing works
Crystal structures of Nova-1 and Nova-2 K-homology RNA-binding domains
Lewis, H. A., Chen, H., Edo, C., Buckanovich, R. J., Yang, Y. Y. L., Musunuru, K., Ru, Z., Darnell, R. B., & Burley, S. K. (1999) · Structure · doi:10.1016/s0969-2126(99)80025-2 · Cited by 108 · see citing works
Self-citationPoint mutation of glycine receptor α1 subunit in the spasmodic mouse affects agonist responses
Saul, B., Schmieden, V., Kling, C., et al. (1994) · FEBS Letters · doi:10.1016/0014-5793(94)00736-5 · Cited by 99 · see citing works
Splicing in the Inner Ear: a Familiar Tune, but What Are the Instruments?
Black, D. L. (1998) · Neuron · doi:10.1016/s0896-6273(00)80444-4 · Cited by 87 · see citing works
MER1, a yeast gene required for chromosome pairing and genetic recombination, is induced in meiosis.
Engebrecht, J., & Roeder, G. S. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.5.2379 · Cited by 77 · see citing works
Ethanol, gamma-aminobutyrate type A receptors, and protein kinase C phosphorylation.
Macdonald, R. L. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.9.3633 · Cited by 73 · see citing works
Serafini, T. A. (1999) · Cell · doi:10.1016/s0092-8674(00)81008-9 · Cited by 69 · see citing works
The inhibitory neuronal glycine receptor
Béchade, C., Sur, C., & Triller, A. (1994) · BioEssays · doi:10.1002/bies.950161008 · Cited by 60 · see citing works
5‐HT4 Receptors: Cloning and Expression of New Splice Variants
Claeysen, S., Faye, P., Sebben, M., Taviaux, S. A., Bockaert, J., & Dumuis, A. (1998) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1998.tb10172.x · Cited by 54 · see citing works
Caviness, J. N. (1996) · Mayo Clinic Proceedings · doi:10.1016/s0025-6196(11)63006-1 · Cited by 51 · see citing works
Meningeal cells can communicate with astrocytes by calcium signaling
Grafstein, B., Liu, S., Cotrina, M. L., Goldman, S. A., & Nedergaard, M. (2000) · Annals of Neurology · doi:10.1002/1531-8249(200001)47:1 · Cited by 50 · see citing works
Essential nucleotides direct neuron-specific splicing of gamma 2 pre-mRNA.
Zhang, L., Ashiya, M., Sherman, T. G., & Grabowski, P. J. (1996) · Europe PMC (PubMed Central) · Cited by 48 · see citing works
Fuks, Z., Alfieri, A. A., Haimovitz‐Friedman, A., et al. (2006) · PubMed · Cited by 41 · see citing works
A unique intronic splicing enhancer controls the inclusion of the agrin Y exon.
Wei, N., Lin, C. Q., Modafferi, E. F., et al. (1997) · PubMed · Cited by 36 · see citing works
Functions of SR and Tra2 Proteins in Pre‐mRNA Splicing Regulation
Tacke, R., & Manley, J. L. (1999) · Proceedings of The Society for Experimental Biology and Medicine · doi:10.1046/j.1525-1373.1999.d01-10.x · Cited by 33 · see citing works
Cell- and stage-specific splicing events resolved in specialized neurons of the rat cerebellum.
Wang, Z., & Grabowski, P. J. (1996) · PubMed · Cited by 28 · see citing works
Averbuch‐Heller, L., & Remler, B. F. (1996) · Seminars in Neurology · doi:10.1055/s-2008-1040955 · Cited by 24 · see citing works
Immunologic Complexity in Neurons
Darnell, R. B. (1998) · Neuron · doi:10.1016/s0896-6273(00)80613-3 · Cited by 23 · see citing works
Self-citationFletcher, C., Okano, H. J., Gilbert, D. J., Yang, Y. Y. L., Yang, C., Copeland, N. G., Jenkins, N. A., & Darnell, R. B. (1997) · Genomics · doi:10.1006/geno.1997.4925 · Cited by 20 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Mechanisms of Alternative Pre-Messenger RNA Splicing
Black, D. L. (2003) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.72.121801.161720 · Cited by 2,704 · see citing works
HITS-CLIP yields genome-wide insights into brain alternative RNA processing
Licatalosi, D. D., Mele, A., Fak, J. J., Ule, J., Kayikci, M., Chi, S. W., Clark, T. A., Schweitzer, A. C., Blume, J. E., Wang, X., et al. (2008) · Nature · doi:10.1038/nature07488 · Cited by 1,352 · see citing works
Self-citationCLIP Identifies Nova-Regulated RNA Networks in the Brain
Ule, J., Jensen, K. B., Ruggiu, M., Mele, A., Ule, A., & Darnell, R. B. (2003) · Science · doi:10.1126/science.1090095 · Cited by 1,120 · see citing works
Self-citationFragile X mental retardation protein targets G quartet mRNAs important for neuronal function
Darnell, J. C., Jensen, K. B., Jin, P., Brown, V., Warren, S. T., & Darnell, R. B. (2001) · Cell · doi:10.1016/s0092-8674(01)00566-9 · Cited by 952 · see citing works
Self-citationVariation in alternative splicing across human tissues
Yeo, G., Holste, D., Kreiman, G., & Burge, C. B. (2004) · Genome Biology · doi:10.1186/gb-2004-5-10-r74 · Cited by 584 · see citing works
Diverse molecular functions of Hu proteins
Hinman, M. N., & Lou, H. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-008-8252-6 · Cited by 587 · see citing works
Alternative splicing in disease and therapy
Garcia-Blanco, M. A., Baraniak, A. P., & Lasda, E. L. (2004) · Nature Biotechnology · doi:10.1038/nbt964 · Cited by 527 · see citing works
Nova regulates brain-specific splicing to shape the synapse
Ule, J., Ule, A., Spencer, J., Williams, A., Hu, J.-S., Cline, M., Wang, H., Clark, T., Fraser, C., Ruggiu, M., et al. (2005) · Nature Genetics · doi:10.1038/ng1610 · Cited by 509 · see citing works
Self-citationRaj, B., & Blencowe, B. J. (2015) · Neuron · doi:10.1016/j.neuron.2015.05.004 · Cited by 475 · see citing works
HITS-CLIP: panoramic views of protein-RNA regulation in living cells
Darnell, R. B. (2010) · WIREs RNA · doi:10.1002/wrna.31 · Cited by 382 · see citing works
Self-citationNeuronal regulation of alternative pre-mRNA splicing
Li, Q., Lee, J.-A., & Black, D. L. (2007) · Nature Reviews Neuroscience · doi:10.1038/nrn2237 · Cited by 376 · see citing works
The neurogenetics of alternative splicing
Vuong, C. K., Black, D. L., & Zheng, S. (2016) · Nature Reviews Neuroscience · doi:10.1038/nrn.2016.27 · Cited by 340 · see citing works
Jin, Y., Suzuki, H., Maegawa, S., Endo, H., Sugano, S., Hashimoto, K., Yasuda, K., & Inoue, K. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg089 · Cited by 311 · see citing works
Darnell, J. C., Fraser, C. E., Mostovetsky, O., Stefani, G., Jones, T. A., Eddy, S. R., & Darnell, R. B. (2005) · Genes & Development · doi:10.1101/gad.1276805 · Cited by 287 · see citing works
Self-citationSpliceAid 2: a database of human splicing factors expression data and RNA target motifs
Piva, F., Giulietti, M., Burini, A. B., & Principato, G. (2012) · Human Mutation · doi:10.1002/humu.21609 · Cited by 256 · see citing works
Analysis of alternative splicing associated with aging and neurodegeneration in the human brain
Tollervey, J. R., Wang, Z., Hortobágyi, T., Witten, J. T., Zarnack, K., Kayikci, M., Clark, T. A., Schweitzer, A. C., Rot, G., Curk, T., et al. (2011) · Genome Research · doi:10.1101/gr.122226.111 · Cited by 252 · see citing works
The end of the message: multiple protein–RNA interactions define the mRNA polyadenylation site
Shi, Y., & Manley, J. L. (2015) · Genes & Development · doi:10.1101/gad.261974.115 · Cited by 249 · see citing works
The splicing regulator Rbfox2 is required for both cerebellar development and mature motor function
Gehman, L. T., Meera, P., Stoilov, P., Shiue, L., O'Brien, J. E., Meisler, M. H., Ares, M., Otis, T. S., & Black, D. L. (2012) · Genes & Development · doi:10.1101/gad.182477.111 · Cited by 213 · see citing works
RNA Protein Interaction in Neurons
Darnell, R. B. (2013) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-062912-114322 · Cited by 181 · see citing works
Self-citationLi, Q., Zheng, S., Han, A., Lin, C.-H., Stoilov, P., Fu, X.-D., & Black, D. L. (2014) · eLife · doi:10.7554/elife.01201 · Cited by 174 · see citing works
McKee, A. E., Minet, E., Stern, C., Riahi, S., Stiles, C. D., & Silver, P. A. (2005) · BMC Developmental Biology · doi:10.1186/1471-213x-5-14 · Cited by 166 · see citing works
Huang, C. S., Shi, S.-H., Ule, J., Ruggiu, M., Barker, L. A., Darnell, R. B., Jan, Y. N., & Jan, L. Y. (2005) · Cell · doi:10.1016/j.cell.2005.07.033 · Cited by 157 · see citing works
Self-citationGenetic Changes Shaping the Human Brain
Bae, B.-I., Jayaraman, D., & Walsh, C. A. (2015) · Developmental Cell · doi:10.1016/j.devcel.2015.01.035 · Cited by 135 · see citing works
Building Robust Transcriptomes with Master Splicing Factors
Jangi, M., & Sharp, P. A. (2014) · Cell · doi:10.1016/j.cell.2014.09.054 · Cited by 141 · see citing works
Nova autoregulation reveals dual functions in neuronal splicing
Dredge, B. K., Stefani, G., Engelhard, C. C., & Darnell, R. B. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600630 · Cited by 125 · see citing works
Self-citationNOVA-dependent regulation of cryptic NMD exons controls synaptic protein levels after seizure
Eom, T., Zhang, C., Wang, H., Lay, K., Fak, J., Noebels, J. L., & Darnell, R. B. (2013) · eLife · doi:10.7554/elife.00178 · Cited by 118 · see citing works
Self-citationRuggiu, M., Herbst, R., Kim, N., Jevsek, M., Fak, J. J., Mann, M. A., Fischbach, G., Burden, S. J., & Darnell, R. B. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0813112106 · Cited by 113 · see citing works
Self-citationA catalog of single nucleotide changes distinguishing modern humans from archaic hominins
Kuhlwilm, M., & Boeckx, C. (2019) · Scientific Reports · doi:10.1038/s41598-019-44877-x · Cited by 108 · see citing works
Minovitsky, S. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki210 · Cited by 103 · see citing works
An intronic VNTR affects splicing of ABCA7 and increases risk of Alzheimer’s disease
On Behalf of the BELNEU Consortium, De Roeck, A., Duchateau, L., Van Dongen, J., Cacace, R., Bjerke, M., Van den Bossche, T., Cras, P., Vandenberghe, R., De Deyn, P. P., et al. (2018) · Acta Neuropathologica · doi:10.1007/s00401-018-1841-z · Cited by 93 · see citing works
ELAV inhibits 3′-end processing to promote neural splicing of <i>ewg</i> pre-mRNA
Soller, M., & White, K. (2003) · Genes & Development · doi:10.1101/gad.1106703 · Cited by 89 · see citing works
Calarco, J. A., Zhen, M., & Blencowe, B. J. (2011) · RNA · doi:10.1261/rna.2603911 · Cited by 72 · see citing works
Leggere, J. C., Saito, Y., Darnell, R. B., Tessier-Lavigne, M., Junge, H. J., & Chen, Z. (2016) · eLife · doi:10.7554/elife.14264 · Cited by 67 · see citing works
Ratti, A., Fallini, C., Colombrita, C., Pascale, A., Laforenza, U., Quattrone, A., & Silani, V. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m706082200 · Cited by 66 · see citing works
Promotion of exon 6 inclusion in HuD pre-mRNA by Hu protein family members
Wang, H., Molfenter, J., Zhu, H., & Lou, H. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq028 · Cited by 49 · see citing works
Cholinergic regulation of striatal Nova mRNAs
Jelen, N., Ule, J., & Živin, M. (2010) · Neuroscience · doi:10.1016/j.neuroscience.2010.05.013 · Cited by 10 · see citing works
- Authors
- Jensen, K. B., Dredge, B. K., Stefani, G., Zhong, R., Buckanovich, R. J., Okano, H. J., Yang, Y. Y. L., & Darnell, R. B.
- Affiliations
- Rockefeller University (US)
- Corresponding author
- Darnell, Robert B
- ORCID
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2000-02
- Volume
- 25
- Issue
- 2
- Pages
- 359-371
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2073654740
- Cited by
- 426
- References
- 83
- Citations by year
- 2026: 8 · 2025: 10 · 2024: 10 · 2023: 18 · 2022: 20 · 2021: 7 · 2020: 11 · 2019: 14 · 2018: 11 · 2017: 16 · 2016: 15 · 2015: 14 · 2014: 18 · 2013: 17 · 2012: 13
- FWCI
- 8.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System (Università degli Studi di Trento) (submittedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (R01 NS34389, R01NS034389)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Health Sciences › Medicine › Neurology › Amyotrophic Lateral Sclerosis Research
- Keywords
- RNA-binding proteins, alternative splicing, neuronal viability, neuronal apoptosis, RNA binding specificity, motor impairment
- MeSH terms
- Neuro-Oncological Ventral Antigen, Animals, Antigens, Neoplasm, Brain Chemistry, Brain Stem, Cell Survival, Exons, Genotype, Mice, Inbred C57BL, Motor Neurons, Nerve Tissue Proteins, Protein Binding, Receptors, GABA-A, Ribonucleoproteins, RNA Precursors, Spinal Cord, RNA-Binding Proteins, Apoptosis, Gene Deletion, Alternative Splicing, Receptors, Glycine, Mice, Knockout, Gene Expression Regulation, Developmental, In Situ Nick-End Labeling, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
