The DOI matches its Crossref record; 28 of the 29 references it cites were found in a registry or an index.
AAV8, 9, Rh10, Rh43 Vector Gene Transfer in the Rat Brain: Effects of Serotype, Promoter and Purification Method
Open accessAbstract
We compared adeno-associated virus (AAV) serotypes for expression levels of green fluorescent protein (GFP) in the adult rat hippocampus by biophotonic imaging. Preparations of AAV serotypes 8, 9, Rh10, and Rh43 incorporating cytomegalovirus (CMV) promoter-driven GFP were purified by a CsCl method. Neither AAV Rh10 nor AAV Rh43 produced greater levels of GFP than AAV8, which was used as a reference. For AAV9, there was an increase relative to AAV8. The CsCl-purified AAV8 displayed an astroglial transduction pattern in contrast to the expected neuronal expression of other AAVs. After preparing the same CMV-GFP plasmid in AAV8 with an iodixanol purification method, the expected neuronal pattern resulted. The astroglial expression with the CsCl AAV8 was probably due to relatively high levels of protein impurities. We compared the CMV promoter with the CMV/chicken beta-actin (CBA) promoter in the context of AAV8, both prepared by iodixanol, and found the CBA promoter to produce stronger GFP expression. At two doses of vectors optimized for serotype, promoter and purification, we did not observe serotype differences among AAV8, AAV9, or AAV Rh10. The purification method can therefore impact the transduction pattern as well as the results when comparing serotype strengths.
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“AAV8 and AAV9 exhibit a dominant neuronal tropism after direct injection into the rodent striatum9,13,16,27 as well as in other brain structures.12,28,29”
From Characterization of a novel adeno-associated viral vector with preferential oligodendrocyte tropism (2016) · cites this paper as [29]
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Chansel‐Debordeaux, L. (2017) · theses.fr (ABES) · doi:10.70675/6b80556ez59a7z4009za535zb2fd974484d2
Achieving cell-type specific transduction with adeno-associated viral vectors in pigeons
Tuff, J. M., Haselhuhn, K. B., Surdin, T., Herlitze, S., Ziegler, M., Güntürkün, O., & Rook, N. (2026) · Current Research in Neurobiology · doi:10.1016/j.crneur.2026.100157
Estrategias de transferencia génica en cerebro mediadas por vectores virales adenoasociados
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Metadata
- Authors
- Klein, R. L., Dayton, R. D., Tatom, J. B., Henderson, K. M., & Henning, P. P.
- Affiliations
- Louisiana State University Health Sciences Center Shreveport (US)
- Corresponding author
- Klein, Ronald L
- ORCID
- Dayton, Robert D
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2008-01
- Volume
- 16
- Issue
- 1
- Pages
- 89-96
- ISSN
- 1525-0016 (ISSN-L)
- PMC
- PMC2987640
- OpenAlex
- W1992976268
- Cited by
- 196
- References
- 29
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 12 · 2023: 9 · 2022: 5 · 2021: 13 · 2020: 8 · 2019: 8 · 2018: 15 · 2017: 4 · 2016: 13 · 2015: 15 · 2014: 15 · 2013: 20 · 2012: 15
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Therapy (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (R01NS048450)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- adeno-associated virus vectors, AAV serotypes, AAV8, AAV9, vector purification, cytomegalovirus promoter, chicken beta-actin promoter, neuronal transduction, hippocampus, GFP expression
- MeSH terms
- Dependovirus, Animals, Brain Chemistry, Cell Line, Cesium, Chlorides, Genetic Vectors, Hippocampus, Humans, Male, Promoter Regions, Genetic, Serotyping, Transduction, Genetic, Triiodobenzoic Acids, Rats, Sprague-Dawley, Hepatitis B Virus, Woodchuck, Green Fluorescent Proteins, Rats
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