Recombinant adeno-associated virus type 2, 4, and 5 vectors: Transduction of variant cell types and regions in the mammalian central nervous system
Abstract
Recombinant adeno-associated virus vectors based on serotype 2 (rAAV2) can direct transgene expression in the central nervous system (CNS), but it is not known how other rAAV serotypes perform as CNS gene transfer vectors. Serotypes 4 and 5 are distinct from rAAV2 and from each other in their capsid regions, suggesting that they may direct binding and entry into different cell types. In this study, we examined the tropisms and transduction efficiencies of β-galactosidase-encoding vectors made from rAAV4 and rAAV5 compared with similarly designed rAAV2-based vectors. Injection of rAAV5 β-galactosidase (βgal) or rAAV4βgal into the lateral ventricle resulted in stable transduction of ependymal cells, with approximately 10-fold more positive cells than in mice injected with rAAV2βgal. Major differences between the three vectors were revealed upon striatal injections. Intrastriatal injection of rAAV4βgal resulted again in striking ependyma-specific expression of transgene, with a notable absence of transduced cells in the parenchyma. rAAV2βgal and rAAV5βgal intrastriatal injections led to β-gal-positive parenchymal cells, but, unlike rAAV2βgal, rAAV5βgal transduced both neurons and astrocytes. The number of transgene-positive cells in rAAV5βgal-injected brains was 130 and 5,000 times higher than in rAAV2βgal-injected brains at 3 and 15 wk, respectively. Moreover, transgene-positive cells were widely dispersed throughout the injected hemisphere in rAAV5βgal-transduced animals. Together, our data provide in vivo support for earlier in vitro work, suggesting that rAAV4 and rAAV5 gain cell entry by means of receptors distinct from rAAV2. These differences could be exploited to improve gene therapy for CNS disorders.
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The only exception is AAV4, which transduces ependymal cells, but not neurons, astrocytes, microglia or oligodendrocytes.
“The only exception is AAV4, which transduces ependymal cells, but not neurons, astrocytes, microglia or oligodendrocytes.8”
From Characterization of a novel adeno-associated viral vector with preferential oligodendrocyte tropism (2016) · cites this paper as [8]
In animal models, widespread CNS coverage can be obtained by delivering the vector into the CSF.
“in animal models, widespread coverage can be obtained by delivering the vector into the CSF”
From AAVrh.10-Mediated APOE2 Central Nervous System Gene Therapy for APOE4-Associated Alzheimer's Disease (2018) · cites this paper as [82]
After intraventricular delivery the vector may be absorbed quickly into the chorionic villi and ependymal cells lining the ventricles.
“the vector may be absorbed quickly into the chorionic villi and ependymal cells lining the ventricles”
From AAVrh.10-Mediated APOE2 Central Nervous System Gene Therapy for APOE4-Associated Alzheimer's Disease (2018) · cites this paper as [82]
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Beutler, A. S. (2010) · Molecular Therapy · doi:10.1038/mt.2010.41 · Cited by 19 · see citing works
- Authors
- Davidson, B. L., Stein, C. S., Heth, J. A., Martins, I., Kotin, R. M., Derksen, T. A., Zabner, J., Ghodsi, A., & Chiorini, J. A.
- Affiliations
- University of Iowa (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2000-02-25
- Volume
- 97
- Issue
- 7
- Pages
- 3428-3432
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W4245307762
- Cited by
- 531
- References
- 35
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 7 · 2023: 11 · 2022: 11 · 2021: 14 · 2020: 9 · 2019: 18 · 2018: 25 · 2017: 16 · 2016: 14 · 2015: 26 · 2014: 22 · 2013: 18 · 2012: 14
- FWCI
- 18.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Funders
- Roy J. Carver Charitable Trust
- National Institutes of Health
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-33531, R01HD033531)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- recombinant adeno-associated virus, AAV2, AAV5, serotype differences, ependymal cells, striatum, lateral ventricle injection, intrastriatal injection, transduction efficiency, cell tropism, beta-galactosidase expression, CNS gene therapy
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