The DOI matches its Crossref record; 47 of the 49 references it cites were found in a registry or an index.
Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP
Abstract
Yeast two-hybrid system was employed to isolate novel proteins that physically interact with PU.1, a member of Ets family transcription factors. Sequence analyses of several isolated clones positive for beta-galactosidase activity revealed that one of these clones was confirmed to encode a transcriptional coactivator, CREB binding protein (CBP). GST binding assay showed that the interacting sites were located at the transcriptional activation domain of PU.1 through 74-122 and the region spanning residues 1283-1915 of CBP. CBP potentiated PU.1-mediated transcription of the reporter gene driven by the multimerized PU.1-binding sites, suggesting that CBP functions as a coactivator for PU.1. Considering that CBP is a limited cellular component to function as a coactivator for several transcription factors, CBP may mediate synergistic and antagonistic interactions between PU.1 and other transcription factors during the process of hematopoietic cell differentiation.
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In the citing paper
“as PU.1 can interact with CBP/p300 (refs 39, 40) and TRIM33”
From TRIM33 switches off Ifnb1 gene transcription during the late phase of macrophage activation (2015) · cites this paper as [39]
In the citing paper
“There is limited understanding of the mechanisms that determine coactivator versus corepressor recruitment by RUNX1 and PU.1.17–20”
From RUNX1 regulates corepressor interactions of PU.1 (2011) · cites this paper as [18]
In the citing paper
“PU.1 can interact with coactivators or corepressors to function as either a transcriptional activator or a transcriptional repressor.17–19”
From RUNX1 regulates corepressor interactions of PU.1 (2011) · cites this paper as [18]
In the citing paper
“However, the mechanisms that regulate corepressor versus coactivator interactions of RUNX1 and PU.1 are incompletely understood.17–20”
From RUNX1 regulates corepressor interactions of PU.1 (2011) · cites this paper as [18]
In the citing paper
“and CBP stimulates PU.1 activity in transient trans- fections (82).”
From Inhibition of CBP-Mediated Protein Acetylation by the Ets Family Oncoprotein PU.1 (2002) · cites this paper as [82]
In the citing paper
“PU.1 is a bifunctional protein as for instance CBP binding leads to PU.1 transcriptional activation [47], whereas TLE-4 was found to support PU.1-dependent repres-sion [46].”
From PKCδ-Induced PU.1 Phosphorylation Promotes Hematopoietic Stem Cell Differentiation to Dendritic Cells (2011) · cites this paper as [47]
In the citing paper
“GST binding assays revealed that a portion of the transcriptional activation domain of PU.1 (amino acids 74–122) directly interacts with the region spanning residues 1283–1915 of CBP (Fig. 4).”
From PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis (2009) · cites this paper as [78]
In the citing paper
“CBP enhances PU.1-mediated transcription of multimerized PU-box luciferase reporter constructs, suggesting that CBP acts as a co-activator for the transcription factor PU.1.”
From PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis (2009) · cites this paper as [78]
In the citing paper
“Because the amount of CBP is limited in cells, CBP may mediate positive and negative cross-talk between PU.1 and other transcription factors in the process of haematopoietic cell differentiation [78].”
From PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis (2009) · cites this paper as [78]
In the citing paper
“In CV-1 cells, but not in NIH 3T3 cells, cooperation between Myb and C/EBP-α depended on the integrity of the PU.1-binding site [78].”
From PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis (2009) · cites this paper as [78]
In the citing paper
“One such candidate is CBP/p300, a co-activator of PU.1 [78], which binds to the transactivation domain of PU.1.”
From PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis (2009) · cites this paper as [78]
In the citing paper
“This activation may in part be due to the recruitment of coactivator/ histone acetylase CBP/p300, since CBP/p300 can interact with PU.1 (but not ICSBP) (32, 59).”
From Gamma Interferon Triggers Interaction between ICSBP (IRF-8) and TEL, Recruiting the Histone Deacetylase HDAC3 to the Interferon-Responsive Element (2002) · cites this paper as [59]
In the citing paper
“This activation seems to involve other factors, notably the coactivator/histone acetylase CBP/p300, with which PU.1 is shown to interact (59).”
From Gamma Interferon Triggers Interaction between ICSBP (IRF-8) and TEL, Recruiting the Histone Deacetylase HDAC3 to the Interferon-Responsive Element (2002) · cites this paper as [59]
Citations
Cites
The Transcriptional Coactivators p300 and CBP Are Histone Acetyltransferases
Ogryzko, V. V., Schiltz, R. L., Russanova, V., Howard, B. H., & Nakatani, Y. (1996) · Cell · doi:10.1016/s0092-8674(00)82001-2 · Cited by 2,952
Activation of p53 Sequence-Specific DNA Binding by Acetylation of the p53 C-Terminal Domain
Gu, W., & Roeder, R. G. (1997) · Cell · doi:10.1016/s0092-8674(00)80521-8 · Cited by 2,559
Kamei, Y., Xu, L., Heinzel, T., Torchia, J., Kurokawa, R., Gloss, B., Lin, S.-C., Heyman, R. A., Rose, D. W., Glass, C. K., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81118-6 · Cited by 2,150
Phosphorylated CREB binds specifically to the nuclear protein CBP
Chrivia, J. C., Kwok, R. P. S., Lamb, N., Hagiwara, M., Montminy, M. R., & Goodman, R. H. (1993) · Nature · doi:10.1038/365855a0 · Cited by 2,052
Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages
Scott, E. W., Simon, M. C., Anastasi, J., & Singh, H. (1994) · Science · doi:10.1126/science.8079170 · Cited by 1,532
Nuclear protein CBP is a coactivator for the transcription factor CREB
Kwok, R. P. S., Lundblad, J. R., Chrivia, J. C., Richards, J. P., Bächinger, H. P., Brennan, R. G., Roberts, S. G. E., Green, M. R., & Goodman, R. H. (1994) · Nature · doi:10.1038/370223a0 · Cited by 1,443
Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities
McKercher, S. R., Torbett, B. E., Anderson, K. L., Henkel, G. W., Vestal, D. J., Baribault, H., Klemsz, M., Feeney, A. J., Wu, G. E., Paige, C. J., et al. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00949.x · Cited by 1,130
The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene
Klemsz, M. J., McKercher, S. R., Celada, A., Van Beveren, C., & Maki, R. A. (1990) · Cell · doi:10.1016/0092-8674(90)90219-5 · Cited by 954
Role of CBP/P300 in nuclear receptor signalling
Chakravarti, D., LaMorte, V. J., Nelson, M. C., Nakajima, T., Schulman, I. G., Juguilon, H., Montminy, M., & Evans, R. M. (1996) · Nature · doi:10.1038/383099a0 · Cited by 932
The Ets family of transcription factors
Wasylyk, B., Hahn, S. L., & Giovane, A. (1993) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1993.tb19864.x · Cited by 839
CREB-binding protein/p300 are transcriptional coactivators of p65
Gerritsen, M. E., Williams, A. J., Neish, A. S., Moore, S., Shi, Y., & Collins, T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.7.2927 · Cited by 820
Transcription Factors, Normal Myeloid Development, and Leukemia
Tenen, D. G., Hromas, R., Licht, J. D., & Zhang, D.-E. (1997) · Blood · doi:10.1182/blood.v90.2.489 · Cited by 735
Activation of cAMP and mitogen responsive genes relies on a common nuclear factor
Arias, J., Alberts, A. S., Brindle, P. K., Claret, F. X., Smeal, T., Karin, M., Feramisco, J. R., & Montminy, M. (1994) · Nature · doi:10.1038/370226a0 · Cited by 726
Regulation of NF-κB by Cyclin-Dependent Kinases Associated with the p300 Coactivator
Perkins, N. D., Felzien, L. K., Betts, J. C., Leung, K., Beach, D., & Nabel, G. J. (1997) · Science · doi:10.1126/science.275.5299.523 · Cited by 720
Shivdasani, R. A., Rosenblatt, M., Zucker‐Franklin, D., Jackson, C. W., Hunt, P., Saris, C. J. M., & Orkin, S. H. (1995) · Cell · doi:10.1016/0092-8674(95)90531-6 · Cited by 700
Synergistic activation of transcription by CBP and p53
Gu, W., Shi, X.-L., & Roeder, R. G. (1997) · Nature · doi:10.1038/42972 · Cited by 599
Lundblad, J. R., Kwok, R. P. S., Laurance, M. E., Harter, M. L., & Goodman, R. H. (1995) · Nature · doi:10.1038/374085a0 · Cited by 588
The transcriptional control of hematopoiesis [see comments]
Shivdasani, R., & Orkin, S. (1996) · Blood · doi:10.1182/blood.v87.10.4025.bloodjournal87104025 · Cited by 560
A family of transcriptional adaptor proteins targeted by the E1A oncoprotein
Arany, Z., Newsome, D., Oldread, E., Livingston, D. M., & Eckner, R. (1995) · Nature · doi:10.1038/374081a0 · Cited by 533
Cooperation of Stat2 and p300/CBP in signalling induced by interferon-α
Bhattacharya, S., Eckner, R., Grossman, S., Oldread, E., Arany, Z., D'Andrea, A., & Livingston, D. M. (1996) · Nature · doi:10.1038/383344a0 · Cited by 493
Two contact regions between Stat1 and CBP/p300 in interferon γ signaling
Zhang, J. J., Vinkemeier, U., Gu, W., Chakravarti, D., Horvath, C. M., & Darnell, J. E., Jr. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.26.15092 · Cited by 487
Pip, a novel IRF family member, is a lymphoid-specific, PU.1-dependent transcriptional activator.
Eisenbeis, C. F., Singh, H., & Storb, U. (1995) · Genes & Development · doi:10.1101/gad.9.11.1377 · Cited by 447
Conjunction dysfunction: CBP/p300 in human disease
Giles, R. H., Peters, D. J. M., & Breuning, M. H. (1998) · Trends in Genetics · doi:10.1016/s0168-9525(98)01438-3 · Cited by 401
Janknecht, R., & Hunter, T. (1996) · Nature · doi:10.1038/383022a0 · Cited by 393
Blobel, G. A., Nakajima, T., Eckner, R., Montminy, M., & Orkin, S. H. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.5.2061 · Cited by 355
CBP as a transcriptional coactivator of c-Myb.Preprint
Dai, P., Akimaru, H., Tanaka, Y., Hou, D., Yasukawa, T., Kanei‐Ishii, C., Takahashi, T., & Ishii, S. (1996) · Genes & Development · doi:10.1101/gad.10.5.528 · Cited by 343
CBP‐induced stimulation of c‐Fos activity is abrogated by E1A.
Bannister, A. J., & Kouzarides, T. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb00157.x · Cited by 337
Kitabayashi, I., Yokoyama, A., Shimizu, K., & Ohki, M. (1998) · The EMBO Journal · doi:10.1093/emboj/17.11.2994 · Cited by 307
Hagemeier, C., Bannister, A. J., Cook, A., & Kouzarides, T. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.4.1580 · Cited by 303
Tanaka, Y., Naruse, I., Maekawa, T., Masuya, H., Shiroishi, T., & Ishii, S. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.19.10215 · Cited by 299
Effect of PU.1 phosphorylation on interaction with NF-EM5 and transcriptional activation
Pongubala, J., Van Beveren, C., Nagulapalli, S., Klemsz, M., McKercher, Maki, R., & Atchison, M. (1993) · Science · doi:10.1126/science.8456286 · Cited by 271 · cited 2×
“PU.1 has been reported to interact with retinoblastoma protein (Rb) and TFIID through the N-terminal region (1 ± 75 aa) (Hagemeier et al., 1993), NF-EM5/Pip through the PEST domain (119 ± 160 aa) (Eisenbeis et al., 1995) and NF-IL6b through the Ets domain (245 ± 272 aa) (Nagulapalli et al., 1995).”
BackgroundNeutrophils and monocytes express high levels of PU.1 (Spi-1) but not Spi-B
Chen, H., Zhang, P., Voso, M., Hohaus, S., Gonzalez, D., Glass, C., Zhang, D., & Tenen, D. (1995) · Blood · doi:10.1182/blood.v85.10.2918.bloodjournal85102918 · Cited by 216
Yang, C., Shapiro, L. H., Rivera, M., Kumar, A., & Brindle, P. K. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.4.2218 · Cited by 212
Oelgeschläger, M., Janknecht, R., Krieg, J., Schreek, S., & Lüscher, B. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00637.x · Cited by 211
Spi-1/PU.1 Transgenic Mice Develop Multistep Erythroleukemias
Moreau-Gachelin, F., Wendling, F., Molina, T., Denis, N., Titeux, M., Grimber, G., Briand, P., Vainchenker, W., & Tavitian, A. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.5.2453 · Cited by 189
Zhang, D., Hohaus, S., Voso, M. T., Chen, H.-M., Smith, L. T., Hetherington, C. J., & Tenen, D. G. (1996) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-85232-9_14 · Cited by 131
Nagulapalli, S., Pongubala, J. M., & Atchison, M. L. (1995) · The Journal of Immunology · doi:10.4049/jimmunol.155.9.4330 · Cited by 110
Spi-1/PU.1: an oncogene of the Ets family
Moreau-Gachelin, F. (1994) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/0304-419x(94)90011-6 · Cited by 110
Cheng, X., Reginato, M. J., Andrews, N. C., & Lazar, M. A. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.3.1407 · Cited by 102
Gong, Q., McDowell, J. C., & Dean, A. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.11.6055 · Cited by 90
Rao, G., Rekhtman, N., Cheng, G., Krasikov, T., & Skoultchi, A. I. (1997) · Oncogene · doi:10.1038/sj.onc.1200807 · Cited by 89
The Ets-Related Transcription Factor PU.1 Immortalizes Erythroblasts
Schuetze, S. M., Stenberg, P. E., & Kabat, D. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.9.5670 · Cited by 88
Yamada, T., Kondoh, N., Matsumoto, M., Yoshida, M., Maekawa, A., & Oikawa, T. (1997) · Blood · doi:10.1182/blood.v89.4.1383 · Cited by 87
Self-citationBellón, T., Perrotti, D., & Calabretta, B. (1997) · Blood · doi:10.1182/blood.v90.5.1828 · Cited by 62
Functional interference between the Spi‐1/PU.1 oncoprotein and steroid hormone or vitamin receptors.
Gauthier, J. M., Bourachot, B., Doucas, V., Yaniv, M., & Moreau-Gachelin, F. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb06203.x · Cited by 58
Kihara‐Negishi, F., Yamada, T., Kubota, Y., Kondoh, N., Yamamoto, H., Abe, M., Shirai, T., Hashimoto, Y., & Oikawa, T. (1998) · International Journal of Cancer · doi:10.1002/(sici)1097-0215(19980518)76:4 · Cited by 32
Self-citationBeug, H., Bauer, A., Dolznig, H., Lindern, M. V., Lobmayer, L., Mellitzer, G., Steinlein, P., Wessely, O., & Müllner, E. (1996) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/s0304-419x(96)00032-7 · Cited by 30
Cited by
Sorted by citations
Molecular biology of the Ets family of transcription factors
Oikawa, T., & Yamada, T. (2003) · Gene · doi:10.1016/s0378-1119(02)01156-3 · Cited by 701
Self-citationRoe, J., Mercan, F., Rivera, K. D., Pappin, D., & Vakoc, C. R. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.04.011 · Cited by 343
“Interestingly, C/EBPa, C/EBPb, PU.1, and MYB have all been shown previously to interact with p300 or CBP (Dai et al., 1996; Mink et al., 1997; Yamamoto et al., 1999).”
BackgroundCREB-binding protein and p300: molecular integrators of hematopoietic transcription
Blobel, G. A. (2000) · Blood · doi:10.1182/blood.v95.3.745.003k05_745_755 · Cited by 257
1 ,25-dihydroxyvitamin D3 is a potent suppressor of interferon -mediated macrophage activation
Helming, L. (2005) · Blood · doi:10.1182/blood-2005-03-1029 · Cited by 252
Heterozygous PU.1 mutations are associated with acute myeloid leukemia
Mueller, B. U., Pabst, T., Osato, M., Asou, N., Johansen, L. M., Minden, M. D., Behre, G., Hiddemann, W., Ito, Y., & Tenen, D. G. (2002) · Blood · doi:10.1182/blood.v100.3.998 · Cited by 243
Metadata
- Authors
- Yamamoto, H., Kihara-Negishi, F., Yamada, T., Hashimoto, Y., & Oikawa, T.
- Affiliations
- Sasaki Institute (JP)
- ORCID
- Yamada, Toshiyuki
- Venue
- Oncogene
- Published
- 1999-02-18
- Volume
- 18
- Issue
- 7
- Pages
- 1495-1501
- ISSN
- 0950-9232 (ISSN-L)
- 1476-5594
- OpenAlex
- W2318149005
- Semantic Scholar
- b28b07fc5cb5ab0b001e5a4822b86f270638d7f1
- Cited by
- 113
- References
- 49
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2021: 2 · 2020: 2 · 2019: 5 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Oncogene (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Keywords
- PU.1, CREB-binding protein, CBP, transcriptional coactivator, ETS transcription factors, protein-protein interaction, transactivation domain, transcriptional activation, hematopoietic differentiation, transcription factor interaction
- MeSH terms
- Proto-Oncogene Protein Spi-1, Animals, Nuclear Proteins, Proto-Oncogene Proteins, Recombinant Fusion Proteins, Trans-Activators, CREB-Binding Protein, Mice
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