A family of transcriptional adaptor proteins targeted by the E1A oncoprotein
Abstract
The cellular protein p300 is a target of the adenoviral E1A oncoprotein and is thought to participate in preventing the G0/G1 transition in the cell cycle, activating certain enhancers and stimulating differentiation pathways. CBP is a protein that is associated with and coactivates the transcription factor CREB, mediating the induction by cyclic AMP of certain responsive promoters. The sequences of p300 and CBP are highly related. We show here that p300, like CBP2, can stimulate transcription. This activity is directly and specifically inhibited by E1A. We also find that CBP exists in a DNA-bound complex containing a member of the CREB family and that E1A and CBP interact with one another in vivo. In keeping with the idea that E1A functionally targets CBP, cAMP-dependent transcription is repressed by E1A. Thus, p300 and CBP define a family of transcriptional adaptor proteins that are specifically targeted by the E1A oncoprotein.
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CBP is a ubiquitous adapter protein that links transcription factors to the basal transcription machinery and is thought to stimulate transcription through contact with RNA polymerase II and histone acetylation.
“CBP is a ubiquitous adapter protein that mediates contacts between transcription factors and the basal transcription machinery (1,26,33) and is thought to stimulate transcription both by physical contact with the RNA polymerase II complex and through intrinsic histone acetylation activity (4, 33, 52).”
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- Authors
- Arany, Z., Newsome, D., Oldread, E., Livingston, D. M., & Eckner, R.
- Affiliations
- Harvard University (US)
- Venue
- Nature
- Published
- 1995-03
- Volume
- 374
- Issue
- 6517
- Pages
- 81-84
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2010605653
- Cited by
- 533
- References
- 28
- Citations by year
- 2025: 1 · 2024: 5 · 2023: 3 · 2022: 2 · 2021: 1 · 2020: 6 · 2019: 8 · 2018: 6 · 2017: 3 · 2016: 6 · 2015: 4 · 2014: 6 · 2013: 5 · 2012: 8
- FWCI
- 20.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- P300, CBP, transcriptional coactivator, CREB, cAMP-dependent transcription, adenovirus E1A, protein-protein interaction, G0/G1 cell cycle arrest, transcriptional repression
- MeSH terms
- Cyclic AMP, DNA, Humans, Nuclear Proteins, Protein Binding, Recombinant Fusion Proteins, Transcription Factors, Transfection, Tumor Cells, Cultured, Transcriptional Activation, Trans-Activators, Adenovirus E1A Proteins, CREB-Binding Protein
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