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Cited by 283

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells

Butz, Karin, Ristriani, Tutik, Hengstermann, Arnd, Denk, Claudia, Scheffner, Martin, Hoppe-Seyler, Felix·2003·Oncogene
DOI 10.1038/sj.onc.1206894
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Abstract

The targeted inhibition of antiapoptotic factors in tumour cells may provide a rational approach towards the development of novel anticancer therapies. Using human papillomavirus (HPV)-transformed cells as a model system, we investigated if RNA interference (RNAi)-mediated gene silencing can be employed in order to overcome the apoptosis resistance of cancer cells. We found that both vector-borne and synthetic small interfering (si)RNAs, specifically directed against the antiapoptotic HPV E6 oncogene, restored dormant tumour suppressor pathways in HPV-positive cancer cells that are otherwise inactive in the presence of E6. This ultimately resulted in massive apoptotic cell death, selectively in HPV-positive tumour cells. These findings show that RNAi provides a powerful molecular strategy to inactivate intracellular E6 function efficiently. Moreover, they define E6 as a most promising therapeutic target to eliminate HPV-positive tumour cells specifically by RNAi. Thus, by sequence-specific targeting of antiapoptotic genes, siRNAs may be developed into novel therapeutics that can efficiently correct the apoptosis deficiency of cancer cells.

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Research works printed but not linked: 40

  1. Agami R . (2002). Curr. Opin. Chem. Biol., 6, 829–834. · DOI
  2. Brummelkamp TR, Bernards R and Agami R . (2002a). Cancer Cell, 2, 243–247. · DOI · Paper page
  3. Brummelkamp TR, Bernards R and Agami R . (2002b). Science, 296, 550–553. · DOI
  4. Butz K, Denk C, Ullmann A, Scheffner M and Hoppe-Seyler F . (2000). Proc. Natl. Acad. Sci. USA, 97, 6693–6697. · DOI
  5. Butz K, Geisen C, Ullmann A, Zentgraf H and Hoppe-Seyler F . (1998). Oncogene, 17, 781–787. · DOI
  6. Chen C and Okayama H . (1987). Mol. Cell. Biol., 7, 2745–2752. · DOI
  7. Chomczynski P and Sacchi N . (1987). Anal. Biochem., 162, 156–159. · DOI
  8. Cole ST and Danos O . (1987). J. Mol. Biol., 193, 599–608. · DOI
  9. Elbashir SM, Harboth J, Lendeckel W, Yalcin A, Weber K and Tuschl T . (2001). Nature, 411, 494–498. · DOI · Paper page
  10. Elbashir SM, Harboth J, Weber K and Tuschl T . (2002). Methods, 26, 199–213. · DOI
  11. El-Deiry WS, Tokino T, Velculescu VE, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW and Vogelstein B . (1993). Cell, 75, 817–825. · DOI · Paper page
  12. Evan GI and Vousden KH . (2001). Nature, 411, 342–348. · DOI · Paper page
  13. Fulda S, Wick W, Weller M and Debatin M . (2002). Nat. Med., 8, 808–815. · DOI
  14. Funk WD, Pak DT, Karas RH, Wright WE and Shay JW . (1992). Mol. Cell. Biol., 12, 2866–2871. · DOI
  15. Goodwin EC and DiMaio D . (2000). Proc. Natl. Acad. Sci. USA, 97, 12513–12518. · DOI · Paper page
  16. Igney FH and Krammer PH . (2002). Nat. Rev. Cancer, 2, 277–288. · DOI
  17. Jaque J-M, Triques K and Stevenson M . (2002). Nature, 418, 435–438. · DOI
  18. Jiang M and Milner J . (2002). Oncogene, 21, 6041–6048. · DOI
  19. Lee NS, Dohjima T, Bauer G, Li H, Li M-J, Ehsani A, Salvaterra P and Rossi J . (2002). Nat. Biotechnol., 19, 500–505. · DOI
  20. Mantovani F and Banks L . (2001). Oncogene, 20, 7874–7887. · DOI
  21. Mittnacht S . (1998). Curr. Opin. Genet. Dev., 8, 21–27. · DOI
  22. Morris MC, Chaloin L, Heitz F and Divita G . (2000). Curr. Opin. Biotechnol., 11, 461–466. · DOI
  23. Münger K, Basile JR, Duensing S, Eichten A, Gonzalez SL, Grace M and Zacny VL . (2001). Oncogene, 20, 7888–7898. · DOI
  24. Nicholson DW . (2000). Nature, 407, 810–816. · DOI
  25. Novina CD, Murray MF, Dykxhoorn DM, Beresford PJ, Riess J, Lee S-K, Collman RG, Lieberman J, Shankar P and Sharp PA . (2002). Nat. Med., 8, 681–686. · DOI
  26. Paddison PJ and Hannon GJ . (2002). Cancer Cell, 2, 17–23. · DOI
  27. Pan H and Griep AE . (1994). Genes Dev., 9, 2157–2169. · DOI
  28. Parkin DM, Bray FI and Devesa SS . (2001). Eur. J. Cancer, 37, S4–S66. · DOI · Paper page
  29. Reed JC . (2001). Trends Mol. Med., 7, 314–319. · DOI
  30. Rittner K, Benavente A, Bompard-Sorlet A, Heitz F, Divita G, Brasseur R and Jacobs E . (2002). Mol. Ther., 5, 104–114. · DOI
  31. Rothbard JB, Garlington S, Lin Q, Kirschberg T, Kreider E, McGrane PL, Wender P and Khavari PA . (2000). Nat. Med., 11, 1253–1257. · DOI
  32. Scheffner M, Werness BA, Huibregtse JM, Levine AJ and Howley PM . (1990). Cell, 63, 1129–1136. · DOI · Paper page
  33. Schneider-Gaedicke A and Schwarz E . (1986). EMBO J., 5, 2285–2292. · DOI
  34. Sedman SA, Barbosa MS, Vass WC, Hubbert NL, Haas JA, Lowy DR and Schiller JT . (1991). J. Virol., 65, 4860–4866. · DOI
  35. Smotkin D, Prokoph H and Wettstein FO . (1989). J. Virol., 63, 1441–1447. · DOI
  36. Thomas M and Banks L . (1998). Oncogene, 17, 2943–2954. · DOI · Paper page
  37. Thomas M and Banks L . (1999). Oncogene, 18, 7690–7700. · DOI
  38. Venturini F, Braspenning J, Homann M, Gissmann L and Sczakiel G . (1999). Nucleic Acids Res., 27, 1585–1592. · DOI
  39. Wells SI, Francis DA, Karpova AY, Dowhanick JJ, Benson JD and Howley PM . (2000). EMBO J., 19, 5762–5771. · DOI
  40. Zur Hausen H . (2002). Nat. Rev. Cancer, 2, 342–350. · DOI

Cited by

Metadata

Authors
Butz, K., Ristriani, T., Hengstermann, A., Denk, C., Scheffner, M., & Hoppe-Seyler, F.
Venue
Oncogene
Publisher
Springer Science and Business Media LLC
Published
2003-09-04
Volume
22
Issue
38
Pages
5938-5945
ISSN
  • 0950-9232
  • 1476-5594
DOI
10.1038/sj.onc.1206894
Cited by
283
References
2
Language
en
Licence

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