MCAK associates with EB1
Abstract
The microtubule (MT)-associated protein EB1 localizes to and promotes growth at MT plus ends. The MT depolymerizing kinesin MCAK has also been reported to track growing MT plus ends. Here, we confirm that human MCAK colocalizes with EB1 at growing MT ends when expressed as a GFP fusion protein in transfected cells. We show that MCAK associates with the C-terminus of EB1 and EB3 but much less efficiently with RP1. EB1 associates with the N-terminal localization and regulatory domain in MCAK but not with the motor domain of the protein. The interaction is competitive with the binding of other EB1 ligands and does not require MTs. Knockdown of EB1 expression using siRNA impaired the ability of GFP-MCAK to localize to MT tips in transfected cells. We propose that MCAK is targeted to growing MT ends by EB1, that MCAK is held in an inactive conformation when associated with EB1 and that this could provide the basis for a mechanism that facilitates rapid switching between phases of MT growth and depolymerization.
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MCAK plus-end tracking requires interaction between MCAK and members of the end-binding protein (EB) family.
“This plus-end tracking behavior requires the interaction between MCAK and members of the end-binding protein (EB) family [5-8]”
From In vitro reconstitution of the functional interplay between MCAK and EB3 at microtubule plus ends (2010) · cites this paper as [6]
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Tracking the ends: a dynamic protein network controls the fate of microtubule tips
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- Authors
- Lee, T., Langford, K. J., Askham, J. M., Brüning-Richardson, A., & Morrison, E. E.
- Affiliations
- St James's University Hospital (GB)
- Corresponding author
- Morrison, E E
- ORCID
- Morrison, E E
- Venue
- Oncogene
- Published
- 2007-10-29
- Volume
- 27
- Issue
- 17
- Pages
- 2494-2500
- ISSN
- 0950-9232 (ISSN-L)
- 1476-5594
- OpenAlex
- W1971219543
- Cited by
- 69
- References
- 26
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 2 · 2023: 2 · 2022: 5 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 1 · 2016: 4 · 2015: 3 · 2014: 6 · 2013: 4 · 2012: 5
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Oncogene (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Cancer Research UK
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
- Physical Sciences › Chemistry › Organic Chemistry › Synthesis and biological activity
- Keywords
- MCAK, EB1, EB3, RP1, microtubule plus ends, microtubule depolymerization, kinesin, protein-protein interaction, microtubule growth, protein localization, siRNA knockdown
- MeSH terms
- Animals, Cell Line, Chlorocebus aethiops, Humans, Ligands, Microtubule-Associated Proteins, Protein Binding, Kinesins
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