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Transcriptional profiling of macrophages derived from monocytes and iPS cells identifies a conserved response to LPS and novel alternative transcription

Alasoo, Kaur, Martinez, Fernando O., Hale, Christine, Gordon, Siamon, Powrie, Fiona, Dougan, Gordon, Mukhopadhyay, Subhankar, Gaffney, Daniel J.·2015·Scientific Reports
DOI 10.1038/srep12524PMC PMC4519778

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Abstract

Macrophages differentiated from human induced pluripotent stem cells (IPSDMs) are a potentially valuable new tool for linking genotype to phenotype in functional studies. However, at a genome-wide level these cells have remained largely uncharacterised. Here, we compared the transcriptomes of naïve and lipopolysaccharide (LPS) stimulated monocyte-derived macrophages (MDMs) and IPSDMs using RNA-Seq. The IPSDM and MDM transcriptomes were broadly similar and exhibited a highly conserved response to LPS. However, there were also significant differences in the expression of genes associated with antigen presentation and tissue remodelling. Furthermore, genes coding for multiple chemokines involved in neutrophil recruitment were more highly expressed in IPSDMs upon LPS stimulation. Additionally, analysing individual transcript expression identified hundreds of genes undergoing alternative promoter and 3′ untranslated region usage following LPS treatment representing a previously under-appreciated level of regulation in the LPS response.

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Cited by

Sorted by citations

Authors
Alasoo, K., Martinez, F. O., Hale, C., Gordon, S., Powrie, F., Dougan, G., Mukhopadhyay, S., & Gaffney, D. J.
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2015-07-30
Volume
5
Issue
1
ISSN
2045-2322
DOI
10.1038/srep12524
PMC
PMC4519778
Cited by
103
References
10
Language
en
Licence

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