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Bacterial diversity in saliva and oral health-related conditions: the Hisayama Study

Takeshita, Toru, Kageyama, Shinya, Furuta, Michiko, Tsuboi, Hidenori, Takeuchi, Kenji, Shibata, Yukie, Shimazaki, Yoshihiro, Akifusa, Sumio, Ninomiya, Toshiharu, Kiyohara, Yutaka, Yamashita, Yoshihisa·2016·Scientific Reports
DOI 10.1038/srep22164PMC PMC4764907
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Abstract

This population-based study determined the salivary microbiota composition of 2,343 adult residents of Hisayama town, Japan, using 16S rRNA gene next-generation high-throughput sequencing. Of 550 identified species-level operational taxonomic units (OTUs), 72 were common, in ≥75% of all individuals, as well as in ≥75% of the individuals in the lowest quintile of phylogenetic diversity (PD). These “core” OTUs constituted 90.9 ± 6.1% of each microbiome. The relative abundance profiles of 22 of the core OTUs with mean relative abundances ≥1% were stratified into community type I and community type II by partitioning around medoids clustering. Multiple regression analysis revealed that a lower PD was associated with better conditions for oral health, including a lower plaque index, absence of decayed teeth, less gingival bleeding, shallower periodontal pockets and not smoking, and was also associated with tooth loss. By contrast, multiple Poisson regression analysis demonstrated that community type II, as characterized by a higher ratio of the nine dominant core OTUs, including Neisseria flavescens, was implicated in younger age, lower body mass index, fewer teeth with caries experience, and not smoking. Our large-scale data analyses reveal variation in the salivary microbiome among Japanese adults and oral health-related conditions associated with the salivary microbiome.

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Metadata

Authors
Takeshita, T., Kageyama, S., Furuta, M., Tsuboi, H., Takeuchi, K., Shibata, Y., Shimazaki, Y., Akifusa, S., Ninomiya, T., Kiyohara, Y., & Yamashita, Y.
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2016-02-24
Volume
6
Issue
1
ISSN
2045-2322
DOI
10.1038/srep22164
PMC
PMC4764907
Cited by
284
References
6
Language
en
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