Exploring flavour-producing core microbiota in multispecies solid-state fermentation of traditional Chinese vinegar
Open accessAbstract
Multispecies solid-state fermentation (MSSF), a natural fermentation process driven by reproducible microbiota, is an important technique to produce traditional fermented foods. Flavours, skeleton of fermented foods, was mostly produced by microbiota in food ecosystem. However, the association between microbiota and flavours and flavour-producing core microbiota are still poorly understood. Here, acetic acid fermentation (AAF) of Zhenjiang aromatic vinegar was taken as a typical case of MSSF. The structural and functional dynamics of microbiota during AAF process was determined by metagenomics and favour analyses. The dominant bacteria and fungi were identified as Acetobacter , Lactobacillus , Aspergillus and Alternaria , respectively. Total 88 flavours including 2 sugars, 9 organic acids, 18 amino acids and 59 volatile flavours were detected during AAF process. O2PLS-based correlation analysis between microbiota succession and flavours dynamics showed bacteria made more contribution to flavour formation than fungi. Seven genera including Acetobacter , Lactobacillus , Enhydrobacter , Lactococcus , Gluconacetobacer , Bacillus and Staphylococcus were determined as functional core microbiota for production of flavours in Zhenjiang aromatic vinegar, based on their dominance and functionality in microbial community. This study provides a perspective for bridging the gap between the phenotype and genotype of ecological system and advances our understanding of MSSF mechanisms in Zhenjiang aromatic vinegar.
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LAB frequently appear in AFP and significantly contribute to the synthesis of vinegar volatile organic compounds, as do non-Saccharomyces like Candida spp., Cryptococcus spp., and Debaryomyces spp.
“In addition to S. cerevisiae, LAB frequently appear in AFP and significantly contribute to the synthesis of vinegar volatile organic compounds, as do non-Saccharomyces like Candida spp., Cryptococcus spp., and Debaryomyces spp. (41, 43).”
From Vinegar: A potential source of healthy and functional food with special reference to sugarcane vinegar (2023) · cites this paper as [41]
The Results sections on microbiota dynamics and microbiota–flavour associations (page unavailable) report that Lactobacillus was prominent during acetic acid fermentation (AAF) and that Lactobacillus and Lactococcus were strongly correlated with volatile flavours. They identify Candida, Cryptococcus and Debaryomyces in vinegar Pei, but do not establish that those yeasts significantly produce volatile compounds; the reported correlations also do not demonstrate synthesis. The study concerns AAF, not an AFP stage. (LLM, conf=0.87)
Experimental — accuracy not yet measuredS. cerevisiae was the predominant yeast species in the AFP of fruit vinegar.
“According to Wang et al. (41), S. cerevisiae was the predominant yeast species in the AFP of fruit vinegar.”
From Vinegar: A potential source of healthy and functional food with special reference to sugarcane vinegar (2023) · cites this paper as [41]
The Results (Phylogenetic landscapes and dynamics of microbiota during AAF process; page unavailable) list Saccharomyces among 21 yeast genera in the acetic acid fermentation of cereal vinegar. They do not identify S. cerevisiae as the predominant yeast species or report on the AFP of fruit vinegar. A further search for fruit vinegar and alcohol fermentation found no passage establishing the claim. (LLM, conf=0.76)
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- Authors
- Wang, Z.-M., Lu, Z.-M., Shi, J.-S., & Xu, Z.-H.
- Affiliations
- Jiangnan University (CN)
- Venue
- Scientific Reports
- Published
- 2016-05-31
- Volume
- 6
- Issue
- 1
- Pages
- 26818-26818
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4886211
- OpenAlex
- W2416898054
- Cited by
- 227
- References
- 48
- Citations by year
- 2026: 5 · 2025: 18 · 2024: 25 · 2023: 45 · 2022: 34 · 2021: 26 · 2020: 29 · 2019: 18 · 2018: 12 · 2017: 14 · 2016: 1
- FWCI
- 18.90
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (2013AA102106, 31530055, No. 31271922, 2012AA021301, 2014AA021501, 31271922)
- National Institute on Alcohol Abuse and Alcoholism (R01AA021301)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Keywords
- acetic acid fermentation, core microbiome, microbial community dynamics, Acetobacter, Lactobacillus, volatile flavor compounds, fermented food microbiota
- MeSH terms
- Acetobacter, Aspergillus, Bacillus, Fermentation, Food Microbiology, Lactobacillus, Staphylococcus, Lactococcus, Acetic Acid, Gluconacetobacter, Metagenomics, Microbiota
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