Elucidating the assembled structure of amphiphiles in solution via cryogenic transmission electron microscopy
Abstract
For the past twenty years, significant progress has been made in both developing cryogenic transmission electron microscopy (cryo-TEM) technology and understanding assembled behavior of amphiphilic molecules. Cryo-TEM can provide high-resolution images of complex fluids in a near state. Samples embedded in a thin layer of vitrified solvent do not exhibit artifacts that would normally occur when using chemical fixation or staining-and-drying techniques. Cryo-TEM has been useful in imaging biological molecules in aqueous solutions. Cryo-TEM has become a powerful tool in the study of -assembled structures of amphiphiles in solution as a complementary tool to small-angle X-ray and neutron scattering, light scattering, rheology measurements, and nuclear magnetic resonance. The application of cryo-TEM in the study of assembled behavior of amphiphilic block copolymers, hydrogels, and other complex soft systems continues to emerge. In this context, the usage of cryo-TEM in the field of amphiphilic complex fluids and self-assembled nano-materials is briefly reviewed, and its unique role in exploring the nature of assembled structure in liquid suspension is highlighted.
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Time-resolved cryo-TEM can efficiently monitor the kinetics of amphiphilic polymers assembly and disassembly in solution, allowing real-time observation of nanosystems formation and disruption.
“the kinetics of amphiphilic polymers assembly and disassembly in solution can be efficiently monitored using timeresolved cryo-TEM, which allows the real-time observation of nanosystems formation and disruption.”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [179]
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- Authors
- Cui, H., Hodgdon, T. K., Kaler, E. W., Abezgauz, L., Danino, D., Lubovsky, M., Talmon, Y., & Pochan, D. J.
- Affiliations
- Delaware Biotechnology Institute (US)
- University of Delaware (US)
- Technion – Israel Institute of Technology (IL)
- Corresponding author
- Pochan, Darrin J.
- Venue
- Soft Matter
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2007
- Volume
- 3
- Issue
- 8
- Pages
- 945
- ISSN
- 1744-683X (ISSN-L)
- 1744-6848
- OpenAlex
- W2118147816
- Cited by
- 208
- References
- 104
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 6 · 2023: 8 · 2022: 3 · 2021: 13 · 2020: 10 · 2019: 7 · 2018: 9 · 2017: 8 · 2016: 10 · 2015: 12 · 2014: 15 · 2013: 9 · 2012: 20
- FWCI
- 14.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Soft Matter (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
- Physical Sciences › Chemistry › Organic Chemistry › Surfactants and Colloidal Systems
- Physical Sciences › Chemistry › Organic Chemistry › Advanced Polymer Synthesis and Characterization
- Keywords
- amphiphilic molecules, self-assembly, cryo-TEM, block copolymers, hydrogels, complex fluids, self-assembled nanomaterials, liquid suspensions
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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