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Cited by 418

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay

Lai, Siyi, Wang, Shengnian, Luo, Jun, Lee, L. James, Yang, Shang-Tian, Madou, Marc J.·2004·Analytical Chemistry

Abstract

This paper presents an integrated microfluidic device on a compact disk (CD) that performs an enzyme-linked immunosorbent assay (ELISA) for rat IgG from a hybridoma cell culture. Centrifugal and capillary forces were used to control the flow sequence of different solutions involved in the ELISA process. The microfluidic device was fabricated on a plastic CD. Each step of the ELISA process was carried out automatically by controlling the rotation speed of the CD. The work on analysis of rat IgG from hybridoma culture showed that the microchip-based ELISA has the same detection range as the conventional method on the 96-well microtiter plate but has advantages such as less reagent consumption and shorter assay time over the conventional method.

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Metadata

Authors
Lai, S., Wang, S., Luo, J., Lee, L. J., Yang, S.-T., & Madou, M. J.
Venue
Analytical Chemistry
Published
2004-02-20
Volume
76
Issue
7
Pages
1832-1837
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
OpenAlex
W2005780072
Cited by
418
References
4
FWCI
19.43
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay. https://app.cochat.ai/papers/10.1021/ac0348322
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Lai, S., Wang, S., Luo, J., Lee, L. J., Yang, S.-T., & Madou, M. J. (2004). Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay. Analytical Chemistry. https://doi.org/10.1021/ac0348322

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.