The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Droplet-based microfluidic system for multicellular tumor spheroid formation and anticancer drug testing
Abstract
Creating multicellular tumor spheroids is critical for characterizing anticancer treatments since it may provide a better model than monolayer culture of tumor cells. Moreover, continuous dynamic perfusion allows the establishment of long term cell culture and subsequent multicellular spheroid formation. A droplet-based microfluidic system was used to form alginate beads with entrapped breast tumor cells. After gelation, the alginate beads were trapped in microsieve structures for cell culture in a continuous perfusion system. The alginate environment permitted cell proliferation and the formation of multicellular spheroids was observed. The dose-dependent response of the tumor spheroids to doxorubicin, and anticancer drug, showed multicellular resistance compared to conventional monolayer culture. The microsieve structures maintain constant location of each bead in the same position throughout the device seeding process, cell proliferation and spheroid formation, treatment with drug, and imaging, permitting temporal and spatial tracking.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The use of 3-d cultures for high-throughput screening: the multicellular spheroid model.
Kunz-Schughart, L. A., Freyer, J. P., Hofstaedter, F., & Ebner, R. (2004) · SLAS DISCOVERY · doi:10.1177/1087057104265040 · Cited by 797
A novel 3D mammalian cell perfusion-culture system in microfluidic channels
Toh, Y.-C., Zhang, C., Zhang, J., Khong, Y. M., Chang, S., Samper, V. D., van Noort, D., Hutmacher, D. W., & Yu, H. (2007) · Lab on a Chip · doi:10.1039/b614872g · Cited by 414
A microfluidic platform for 3-dimensional cell culture and cell-based assays
Kim, M. S., Yeon, J. H., & Park, J.-K. (2007) · Biomedical Microdevices · doi:10.1007/s10544-006-9016-4 · Cited by 166
MDA435/LCC6 and MDA435/LCC6MDR1: ascites models of human breast cancer
Leonessa, F., Green, D., Licht, T., Wright, A., Wingate-Legette, K., Lippman, J., Gottesman, M., & Clarke, R. (1996) · British Journal of Cancer · doi:10.1038/bjc.1996.29 · Cited by 102
Cited by
Microfluidic fabrication of microparticles for biomedical applications
Li, W., Zhang, L., Ge, X., Xu, B., Zhang, W., Qu, L., Choi, C.-H., Xu, J., Zhang, A., Lee, H., et al. (2018) · Chemical Society Reviews · doi:10.1039/c7cs00263g · Cited by 585
Organ-on-a-chip platforms for studying drug delivery systems
Bhise, N. S., Ribas, J., Manoharan, V., Zhang, Y. S., Polini, A., Massa, S., Dokmeci, M. R., & Khademhosseini, A. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.05.004 · Cited by 381
Microfluidics for cell-based high throughput screening platforms—A review
Du, G., Fang, Q., & den Toonder, J. M. J. (2016) · Analytica Chimica Acta · doi:10.1016/j.aca.2015.11.023 · Cited by 276
Micro Total Analysis Systems for Cell Biology and Biochemical Assays
Kovarik, M. L., Gach, P. C., Ornoff, D. M., Wang, Y., Balowski, J., Farrag, L., & Allbritton, N. L. (2012) · Analytical Chemistry · doi:10.1021/ac202611x · Cited by 270
Moshksayan, K., Kashaninejad, N., Warkiani, M. E., Lock, J. G., Moghadas, H., Firoozabadi, B., Saidi, M. S., & Nguyen, N.-T. (2018) · Sensors and Actuators B: Chemical · doi:10.1016/j.snb.2018.01.223 · Cited by 267
Metadata
- Authors
- Yu, L., Chen, M. C. W., & Cheung, K. C.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2010
- Volume
- 10
- Issue
- 18
- Pages
- 2424
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2126750664
- Cited by
- 247
- References
- 4
- FWCI
- 6.92
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Droplet-based microfluidic system for multicellular tumor spheroid formation and anticancer drug testing. https://app.cochat.ai/papers/10.1039/c004590j
- The paper
- Yu, L., Chen, M. C. W., & Cheung, K. C. (2010). Droplet-based microfluidic system for multicellular tumor spheroid formation and anticancer drug testing. Lab on a Chip. https://doi.org/10.1039/c004590j
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
