A microfluidic platform for chemoresistive testing of multicellular pleural cancer spheroids
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This study reports on a microfluidic platform on which single multicellular spheroids from malignant pleural mesothelioma (MPM), an aggressive tumor with poor prognosis, can be loaded, trapped and tested for chemotherapeutic drug response.
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Continuously perfused spheroids exhibited higher chemoresistance to cisplatin compared with spheroids cultured under static conditions.
“Remarkably, continuously perfused spheroids exhibited higher chemoresistance to cisplatin, a drug commonly prescribed for MPM patients, when compared with spheroids cultured under static conditions.”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [20]
Ruppen et al. trapped MPM cancer spheroids within a multi-S-shaped microfluidic channel and perfused culture medium and anticancer drug formulations through this system.
“These authors trapped cancer spheroids derived from malignant pleural mesothelioma (MPM) cells within a “multi-S-shaped” microfluidic channel (Figure 3a) and perfused culture medium and anticancer drug formulations through this system.”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [20]
Spheroid morphology was similar in both perfused and static conditions and remained essentially unchanged during a 48-h perfusion period.
“The authors observed that the spheroid morphology was similar in both the perfused and the static conditions and remained essentially unchanged during a 48-h perfusion period.”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [20]
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- Authors
- Ruppen, J., Cortes-Dericks, L., Marconi, E., Karoubi, G., Schmid, R. A., Peng, R., Marti, T. M., & Guenat, O. T.
- Affiliations
- University of Bern (CH)
- Division of General Thoracic Surgery
- Corresponding author
- Guenat, Olivier T.
- Venue
- Lab Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2014
- Volume
- 14
- Issue
- 6
- Pages
- 1198-1205
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2079761432
- Cited by
- 105
- References
- 36
- Citations by year
- 2025: 5 · 2024: 10 · 2023: 4 · 2022: 7 · 2021: 18 · 2020: 10 · 2019: 8 · 2018: 8 · 2017: 11 · 2016: 7 · 2015: 14 · 2014: 3
- FWCI
- 6.06
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- Lab on a Chip (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Bern Open Repository and Information System (University of Bern) (submittedVersion, cc-by)
- Open Access CRIS of the University of Bern
- Funders
- Novartis Foundation
- École Polytechnique Fédérale de Lausanne
- Novartis Stiftung für Medizinisch-Biologische Forschung
- Bernische Krebsliga
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Keywords
- malignant pleural mesothelioma, tumor spheroids, chemoresistance, cisplatin, microfluidic perfusion, caspase activity, spheroid viability, drug susceptibility testing, personalized medicine, continuous perfusion
- MeSH terms
- Drug Screening Assays, Antitumor, Humans, Mesothelioma, Pleural Neoplasms, Spheroids, Cellular, Cell Line, Tumor, Microfluidic Analytical Techniques
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