One-step gene delivery into the cytoplasm in a fusion-dependent manner based on a new membrane fusogenic lipid
Abstract
A new type of membrane fusogenic lipid was prepared to deliver DNA or siRNA into the cytoplasm directly in a fusion-dependent manner in order to bypass the cellular endocytosis to avoid the inefficient escape from the endosome and low transfection efficacy.
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Direct transmembrane delivery of particles into cytosol bypassing endo-lysosomes may be alternative way.
“In fact, direct transmembrane delivery of the particles into the cytosol bypassing the endo-lysosomes may be an alternative way [187, 188].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [187]
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Advances in lipid-based carriers for efficient cytosolic delivery via membrane fusion
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- Authors
- Deng, H., Zhao, X., He, D., Guo, W., Yang, K., Dong, A., & Liang, X.-J.
- Affiliations
- Tianjin University (CN)
- Chinese Academy of Sciences (CN)
- Corresponding author
- Dong, Anjie
- Liang, Xing-Jie
- Venue
- Chemical Communications
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2016
- Volume
- 52
- Issue
- 46
- Pages
- 7406-7408
- ISSN
- 1359-7345 (ISSN-L)
- 1364-548X
- OpenAlex
- W2344234162
- Cited by
- 9
- References
- 18
- Citations by year
- 2026: 1 · 2023: 1 · 2020: 2 · 2018: 1 · 2017: 4
- FWCI
- 0.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemical Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31470963, 81371667)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- gene delivery, cytoplasmic delivery, DNA transfection, siRNA delivery, endosomal escape
- MeSH terms
- Endocytosis, Humans, Membrane Fusion, Membrane Lipids, Genetic Therapy, Gene Transfer Techniques
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