Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing
Abstract
Direct cellular delivery of CRISPR/Cas9 complexes is of great significance for genome editing and other recently developed applications, such as gene expression regulation and RNA/DNA imaging. Here, we first constructed a graphene oxide (GO)-polyethylene glycol (PEG)-polyethylenimine (PEI) nanocarrier for the delivery of high-molecular-weight Cas9/single-guide RNA (sgRNA) complexes for endocytosis, endosomal escape, nuclear entry, and gene editing. The results demonstrate that the nanocarrier can be used successfully for efficient gene editing in human AGS cells with an efficiency of ∼39%. The results also show that this nanocarrier can protect sgRNA from enzymatic degradation, thus exhibiting extremely high stability, which is critical for future in vivo applications. Thus, this GO-mediated Cas9/sgRNA delivery system has potential as a new approach for biomedical research and targeted gene engineering applications.
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Zeolitic imidazole frameworks and graphene oxide-PEG-polyethylenimine have been investigated to facilitate endosomal evasion.
“Some materials, such as zeolitic imidazole frameworks (ZIFs) [229] and graphene oxide (GO)-PEG-polyethylenimine (PEI) [230] have been investigated to facilitate endosomal evasion.”
From RNA delivery biomaterials for the treatment of genetic and rare diseases (2019) · cites this paper as [230]
Graphene oxide-poly(ethylene glycol)-polyethylenimine (GO-PEG-PEI) nanocarrier induced efficient genome editing in AGS cells with an efficiency of ~39%.
“In a recent study, graphene oxide-poly(ethylene glycol)-polyethylenimine (GO-PEG-PEI) nanocarrier mediated efficient CRISPR RNPs delivery via physisorption and π-stacking interaction. This strategy induced efficient genome editing in AGS cells with an efficiency of ~39% (Fig. 2D) [102].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [102]
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- Authors
- Yue, H., Zhou, X., Cheng, M., & Xing, D.
- Affiliations
- South China Normal University (CN)
- Corresponding author
- Zhou, Xiaoming
- Xing, Da
- Venue
- Nanoscale
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2018
- Volume
- 10
- Issue
- 3
- Pages
- 1063-1071
- ISSN
- 2040-3364 (ISSN-L)
- 2040-3372
- OpenAlex
- W2778181726
- Cited by
- 150
- References
- 47
- Citations by year
- 2026: 4 · 2025: 13 · 2024: 12 · 2023: 14 · 2022: 22 · 2021: 23 · 2020: 23 · 2019: 25 · 2018: 13 · 2012: 1
- FWCI
- 6.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nanoscale (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (21475048)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Physical Sciences › Engineering › Biomedical Engineering › Graphene and Nanomaterials Applications
- Keywords
- graphene oxide, CRISPR/Cas9 delivery, single guide RNA, polyethylene glycol, polyethyleneimine, nanocarriers, genome editing, endosomal escape, nuclear entry, AGS cells
- MeSH terms
- Gene Editing, RNA, Guide, CRISPR-Cas Systems, Cell Line, Graphite, Humans, Oxides, RNA, Guide, Kinetoplastida, Green Fluorescent Proteins, CRISPR-Cas Systems
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