Designing well-defined photopolymerized synthetic matrices for three-dimensional culture and differentiation of induced pluripotent stem cells
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In this work, we establish an approach for the three-dimensional (3D) culture and differentiation of induced pluripotent stem cells (iPSCs) within well-defined synthetic matrices using light-based chemistries of relevance for the study of both disease and regeneration.
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PEG gels functionalized with YIGSR and PHSRNG10RGDS were permissive for human iPSC survival and differentiation into neural progenitor cells in 3D culture.
“The authors found that PEG gels functionalized with YIGSR and PHSRNG10RGDS were permissive for human iPSC survival and differentiation into neural progenitor cells in 3D culture.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [161]
Ovadia and colleagues compared photo-crosslinked PEG-based gels that contained chemical cues such as the laminin-derived cell-binding sequences YIGSR and IKVAV, the fibronectin-derived sequences PHSRNG10RGDS and RGDS, and the vitronectin-derived sequence KKQRFRHRNRKG.
“To promote iPSC differentiation into human neuronal progenitor cells, Ovadia and colleagues compared photo-crosslinked PEG-based gels that contained chemical cues such as the laminin-derived cell-binding sequences YIGSR and IKVAV, the fibronectin-derived sequences PHSRNG10RGDS and RGDS, and the vitronectin-derived sequence KKQRFRHRNRKG161.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [161]
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- Authors
- Ovadia, E. M., Colby, D. W., & Kloxin, A. M.
- Affiliations
- University of Delaware (US)
- Corresponding author
- Kloxin, April M.
- Venue
- Biomaterials Science
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2018
- Volume
- 6
- Issue
- 6
- Pages
- 1358-1370
- ISSN
- 2047-4830 (ISSN-L)
- 2047-4849
- PMC
- PMC6126667
- OpenAlex
- W2796682556
- Cited by
- 40
- References
- 66
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 5 · 2023: 4 · 2022: 5 · 2021: 5 · 2020: 3 · 2019: 7 · 2018: 1
- FWCI
- 1.51
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials Science (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of General Medical Sciences (NIGMS P20GM104316, 5 P30 GM110758-02)
- Susan G. Komen (CCR16377327)
- PEW Charitable Trusts (0026178)
- Burroughs Wellcome Fund (1006787)
- University of Delaware Research Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- induced pluripotent stem cells, synthetic hydrogels, photopolymerization, peptide hydrogels, 3D cell culture, cell differentiation, neural progenitor cells, integrin-binding motif, beta1 integrin, cell-matrix interactions, cell viability, cell clustering, disease models
- MeSH terms
- Biocompatible Materials, Cell Differentiation, Cell Line, Cell Survival, Cross-Linking Reagents, Humans, Norbornanes, Peptides, Polyethylene Glycols, Cells, Immobilized, Cell Culture Techniques, Hydrogels, Neurogenesis, Photochemical Processes, Induced Pluripotent Stem Cells, Polymerization
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