Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties
Open accessAbstract
We report a strategy to create photodegradable poly(ethylene glycol)–based hydrogels through rapid polymerization of cytocompatible macromers for remote manipulation of gel properties in situ. Postgelation control of the gel properties was demonstrated to introduce temporal changes, creation of arbitrarily shaped features, and on-demand pendant functionality release. Channels photodegraded within a hydrogel containing encapsulated cells allow cell migration. Temporal variation of the biochemical gel composition was used to influence chondrogenic differentiation of encapsulated stem cells. Photodegradable gels that allow real-time manipulation of material properties or chemistry provide dynamic environments with the scope to answer fundamental questions about material regulation of live cell function and may affect an array of applications from design of drug delivery vehicles to tissue engineering systems.
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The viscoelastic profile of hydrogels defines the mechanical confinement of growing organoids.
“The viscoelastic profile of hydrogels has also been shown to define the mechanical confinement of growing organoids66.”
From Organoids (2022) · cites this paper as [66]
The passage on gel crosslinking density (indexed under Abstract; no page given) shows that densely crosslinked hydrogels kept encapsulated human mesenchymal stem cells rounded, while reducing crosslinking allowed them to spread. This supports a role for gel structure in mechanically restricting cells, but the paper does not establish that a hydrogel’s viscoelastic profile defines the confinement of growing organoids. (LLM, conf=0.83)
Experimental — accuracy not yet measuredManipulating synthetic hydrogels using methods such as electrospinning, photopatterning, spraying of microspheres, inkjet and 3D printing, or microfluidic channels enables control over the shape and size of the organoids.
“Manipulating synthetic hydrogels using methods such as electrospinning125, photopatterning126,127, spraying of microspheres128, inkjet and 3D printing129, or microfluidic channels130 further enables control over the shape and size of the organoids.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [126]
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Self-citation
- Authors
- Kloxin, A. M., Kasko, A. M., Salinas, C. N., & Anseth, K. S.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Kasko, Andrea M.
- Anseth, Kristi S.
- Venue
- Science
- Published
- 2009-04-03
- Volume
- 324
- Issue
- 5923
- Pages
- 59-63
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC2756032
- OpenAlex
- W2067897176
- Cited by
- 1,748
- References
- 34
- Citations by year
- 2026: 62 · 2025: 69 · 2024: 60 · 2023: 84 · 2022: 78 · 2021: 122 · 2020: 127 · 2019: 104 · 2018: 126 · 2017: 128 · 2016: 118 · 2015: 107 · 2014: 134 · 2013: 124 · 2012: 120
- FWCI
- 48.26
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institute of Dental and Craniofacial Research (R01DE012998, DE16523, DE12998, R01DE016523)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Chemistry › Organic Chemistry › Photopolymerization techniques and applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- photodegradable hydrogels, poly(ethylene glycol) hydrogels, dynamic hydrogels, cell migration, chondrogenic differentiation, stem cells, drug carriers, tissue engineering, encapsulated cells
- MeSH terms
- Acrylates, Cell Differentiation, Cell Movement, Cell Survival, Cross-Linking Reagents, Humans, Light, Oligopeptides, Photochemistry, Photolysis, Polyethylene Glycols, Polymers, Chondrocytes, Hydrogels, Chondrogenesis, Collagen Type II, Cell Line, Tumor, Mesenchymal Stem Cells
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