Cited by 308Hydrogels: synthesis, properties, applicationsCellular Mechanics and Interactions3D Printing in Biomedical Research

In situ cell manipulation through enzymatic hydrogel photopatterning

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Mosiewicz, Katarzyna A., Kolb, Laura, van der Vlies, André J., Martino, Mikaël M., Lienemann, Philipp S., Hubbell, Jeffrey A., Ehrbar, Martin, Lutolf, Matthias P.·2013·Nature Materials

Abstract

The physicochemical properties of hydrogels can be manipulated in both space and time through the controlled application of a light beam. However, methods for hydrogel photopatterning either fail to maintain the bioactivity of fragile proteins and are thus limited to short peptides, or have been used in hydrogels that often do not support three-dimensional (3D) cell growth. Here, we show that the 3D invasion of primary human mesenchymal stem cells can be spatiotemporally controlled by micropatterning the hydrogel with desired extracellular matrix (ECM) proteins and growth factors. A peptide substrate of activated transglutaminase factor XIII (FXIIIa)--a key ECM crosslinking enzyme--is rendered photosensitive by masking its active site with a photolabile cage group. Covalent incorporation of the caged FXIIIa substrate into poly(ethylene glycol) hydrogels and subsequent laser-scanning lithography affords highly localized biomolecule tethering. This approach for the 3D manipulation of cells within gels should open up avenues for the study and manipulation of cell signalling.

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  • PEG-based matrices responsive to physical or chemical stimuli have been created to reproduce the dynamics of the reparative process by modulating the local milieu in time and space.

    “PEG-based matrices that are responsive to physical (light) or chemical (enzymes) stimuli have been created to reproduce the dynamics of the reparative process, by modulating the local milieu in time and space (146).”
  • Synthetic hydrogels can be functionalized with biologically active moieties that permit the growth and spread of cells; concentration and spacing of these cues can be changed independently.

    “Synthetic hydrogels can be functionalized with biologically active moieties that permit the growth and spread of cells146–151; concentration and spacing of these cues can be changed independently152–155.”

    From Towards organoid culture without Matrigel (2021) · cites this paper as [154]

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Authors
Mosiewicz, K. A., Kolb, L., van der Vlies, A. J., Martino, M. M., Lienemann, P. S., Hubbell, J. A., Ehrbar, M., & Lutolf, M. P.
Affiliations
  • École Polytechnique Fédérale de Lausanne (CH)
  • Immunology Frontier Research Center, The University of Osaka (JP)
  • University of Zurich (CH)
Venue
Nature Materials
Published
2013-10-13
Volume
12
Issue
11
Pages
1072-1078
ISSN
  • 1476-1122 (ISSN-L)
  • 1476-4660
OpenAlex
W2099164785
Cited by
308
References
36
Citations by year
2026: 2 · 2025: 5 · 2024: 11 · 2023: 18 · 2022: 20 · 2021: 22 · 2020: 28 · 2019: 18 · 2018: 34 · 2017: 38 · 2016: 43 · 2015: 37 · 2014: 27 · 2013: 4 · 2012: 1
FWCI
17.04
Type
journal article
Language
en
Open access
Locations
  • Nature Materials (publishedVersion)
  • PubMed (publishedVersion)
  • Zurich Open Repository and Archive (University of Zurich) (acceptedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
  • Zurich Open Repository and Archive (University of Zurich) (submittedVersion)
  • Zurich Open Repository and Archive (University of Zurich)
Funders
École Polytechnique Fédérale de Lausanne
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
enzymatic crosslinking, photolabile protecting groups, poly(ethylene glycol) hydrogels, mesenchymal stem cells, extracellular matrix proteins, growth factor immobilization, signal transduction
MeSH terms
Amino Acid Sequence, Animals, Enzymes, Immobilized, Humans, Light, Photolysis, Polyethylene Glycols, Rabbits, Hydrogels, Factor XIIIa, Mice, Microtechnology, Mesenchymal Stem Cells, Cell Engineering

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