Cited by 28Gold and Silver Nanoparticles Synthesis and ApplicationsSpectroscopy Techniques in Biomedical and Chemical ResearchNanoplatforms for cancer theranostics

Multiplexed molecular imaging with surface enhanced resonance Raman scattering nanoprobes reveals immunotherapy response in mice via multichannel image segmentation

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Andreou, Chrysafis, Plakas, Konstantinos, Berisha, Naxhije, Gigoux, Mathieu, Rosch, Lauren E., Mirsafavi, Rustin, Oseledchyk, Anton, Pal, Suchetan +4 more·2022·Nanoscale Horizons

Abstract

Multiplexed imaging of 8 molecular targets with SERS nanoprobes in mice reveals spatially heterogeneous tumor responses to immunotherapy. Multichannel image segmentation enables classification of the tumor regions into responders and naïve.

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  1. [11]Stack EC; Wang CC; Roman KA; Hoyt CC, Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods 2014, 70 (1), 46– 58. 25242720

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Authors
Andreou, C., Plakas, K., Berisha, N., Gigoux, M., Rosch, L. E., Mirsafavi, R., Oseledchyk, A., Pal, S., Zamarin, D., Merghoub, T., Detty, M. R., & Kircher, M. F.
Affiliations
  • Memorial Sloan Kettering Cancer Center (US)
  • University at Buffalo, State University of New York (US)
  • The Graduate Center, CUNY (US)
  • Brigham and Women's Hospital (US)
Venue
Nanoscale Horizons
Published
2022
Volume
7
Issue
12
Pages
1540-1552
ISSN
  • 2055-6756 (ISSN-L)
  • 2055-6764
OpenAlex
W4307336208
Cited by
28
References
50
Citations by year
2026: 9 · 2025: 8 · 2024: 10 · 2023: 1
FWCI
1.09
Type
journal article
Language
en
Open access
Locations
  • Nanoscale Horizons (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Pershing Square Sohn Cancer Research Alliance (Pershing Square Sohn Prize)
  • Memorial Sloan-Kettering Cancer Center (Center for Molecular Imaging & Nanotechnology (CMI, Technology Development Grants, P30-CA008748)
  • Damon Runyon Cancer Research Foundation (Rachleff Innovation Award DRR-29-14)
Topics
  • Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
Keywords
surface-enhanced Raman scattering, SERS nanoprobes, Raman imaging, immune checkpoint blockade, immunotherapy response, tumor microenvironment, superpixel segmentation, machine learning classification, treatment response prediction, treatment resistance, tumor heterogeneity
MeSH terms
Animals, Antibodies, Immunologic Factors, Immunotherapy, Neoplasms, Spectrum Analysis, Raman, Mice, Molecular Imaging, Tumor Microenvironment

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