Competition between Escherichia coli strains expressing either a periplasmic or a membrane-bound nitrate reductase: does Nap confer a selective advantage during nitrate-limited growth?
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The physiological role of the periplasmic nitrate reductase, Nap, one of the three nitrate reductases synthesized by Escherichia coli K-12, has been investigated. A series of double mutants that express only one nitrate reductase were grown anaerobically in batch cultures with glycerol as the non-fermentable carbon source and nitrate as the terminal electron acceptor. Only the strain expressing nitrate reductase A grew rapidly under these conditions. Introduction of a narL mutation severely decreased the growth rate of the nitrate reductase A strain, but enhanced the growth of the Nap+ strain. The ability to use nitrate as a terminal electron acceptor for anaerobic growth is therefore regulated primarily by the NarL protein at the level of transcription. Furthermore, the strain expressing nitrate reductase A had a substantial selective advantage in competition with the strain expressing only Nap during nitrate-sufficient continuous culture. However, the strain expressing Nap was preferentially selected during nitrate-limited continuous growth. The saturation constants for nitrate for the two strains (which numerically are equal to the nitrate concentrations at half of the maximum specific growth rate and therefore reflect the relative affinities for nitrate) were estimated using the integrated Monod equation to be 15 and 50 μM for Nap and nitrate reductase A respectively. This difference is sufficient to explain the selective advantage of the Nap+ strain during nitrate-limited growth. It is concluded that one physiological role of the periplasmic nitrate reductase of enteric bacteria is to enable bacteria to scavenge nitrate in nitrate-limited environments.
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NapB is an essential component of electron transport in organisms where it is present.
“This small c-type cytochrome is an essential component of electron transport in organisms where it is present.101,105”
From Nitrate and periplasmic nitrate reductases (2014) · cites this paper as [101]
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- Authors
- POTTER, L. C., MILLINGTON, P., GRIFFITHS, L., THOMAS, G. H., & COLE, J. A.
- Affiliations
- University of Birmingham (GB)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1999-11-08
- Volume
- 344
- Issue
- 1
- Pages
- 77-84
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1220616
- OpenAlex
- W4238414190
- Cited by
- 160
- References
- 6
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 8 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 6 · 2019: 9 · 2018: 3 · 2017: 3 · 2016: 6 · 2015: 10 · 2014: 7 · 2013: 7 · 2012: 5
- FWCI
- 3.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Directorate for Biological Sciences
- Medical Research Council
- Biotechnology and Biological Sciences Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
- Keywords
- Escherichia coli, periplasmic nitrate reductase, NAP, competitive growth, anaerobic nitrate respiration, glycerol carbon source
- MeSH terms
- Anaerobiosis, Base Sequence, Cell Membrane, Electron Transport, Escherichia coli, Genes, Bacterial, Kinetics, Mutation, Nitrate Reductases, DNA Primers, Nitric Acid, Periplasm, Nitrate Reductase
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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