Cited by 84Fungal and yeast genetics researchCholesterol and Lipid MetabolismLipid metabolism and biosynthesis

SUT1-promoted sterol uptake involves the ABC transporter Aus1 and the mannoprotein Dan1 whose synergistic action is sufficient for this process

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ALIMARDANI, Parissa, RÉGNACQ, Matthieu, MOREAU-VAUZELLE, Carole, FERREIRA, Thierry, ROSSIGNOL, Tristan, BLONDIN, Bruno, BERGÈS, Thierry·2004·Biochemical Journal

Abstract

Efficient sterol influx in the yeast Saccharomyces cerevisiae is restricted to anaerobiosis or to haem deficiency resulting from mutations. Constitutive expression of SUT1, an hypoxic gene encoding a transcriptional regulator, induces sterol uptake in aerobiosis. A genome-wide approach using DNA microarray was used to identify the mediators of SUT1 effects on aerobic sterol uptake. A total of 121 ORFs (open reading frames) were significantly and differentially expressed after SUT1 overexpression, 61 down-regulated and 60 up-regulated. Among these genes, the role of the putative ABC transporter (ATP-binding-cassette transporter) Aus1, and of the cell-wall mannoprotein Dan1, was characterized better. These two genes play an essential role in aerobic sterol uptake, since their deletion compromised the SUT1 effects, but individual overexpression of either of these genes in a wild-type background was not sufficient for this process. However, constitutive co-expression of AUS1 and DAN1 in a wild-type background resulted in sterol influx in aerobiosis. These results suggest that the corresponding proteins may act synergistically in vivo to promote sterol uptake.

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  • The overexpression of DAN1 and AUS1 is sufficient to promote the uptake of sterol in aerobiosis.

    “In fact, the overexpression of DAN1 and AUS1 is sufficient to promote the uptake of sterol in aerobiosis [105].”

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Authors
ALIMARDANI, P., RÉGNACQ, M., MOREAU-VAUZELLE, C., FERREIRA, T., ROSSIGNOL, T., BLONDIN, B., & BERGÈS, T.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
Corresponding author
  • ALIMARDANI, Parissa
  • FERREIRA, Thierry
Venue
Biochemical Journal
Published
2004-06-22
Volume
381
Issue
1
Pages
195-202
ISSN
  • 0264-6021 (ISSN-L)
  • 1470-8728
OpenAlex
W2013026032
Cited by
84
References
33
Citations by year
2026: 2 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 5 · 2015: 2 · 2014: 4 · 2013: 5 · 2012: 5
FWCI
2.14
Type
journal article
Language
en
Open access
Locations
  • Biochemical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
sterol uptake, SUT1, ABC transporters, mannoproteins, Saccharomyces cerevisiae, transcription factors, cell wall
MeSH terms
Aerobiosis, DNA-Binding Proteins, Glycoproteins, Membrane Glycoproteins, Microtubule-Associated Proteins, Repressor Proteins, Saccharomyces cerevisiae, Species Specificity, Sterols, Transcription, Genetic, Trans-Activators, Gene Expression Regulation, Fungal, Genome, Fungal, ATP-Binding Cassette Transporters, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Saccharomyces cerevisiae Proteins

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