The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4 P and PtdIns(4,5) P 2
Open accessAbstract
PtdIns4P is the major precursor for the synthesis of the multifunctional plasma membrane lipid, PtdIns(4,5)P2. Yet PtdIns4P also functions as a regulatory lipid in its own right, particularly at the Golgi apparatus. In the present study we define specific conditions that enable preservation of several organellar membranes for the immunocytochemical detection of PtdIns4P. We report distinct pools of this lipid in both Golgi and plasma membranes, which are synthesized by different PI4K (phosphatidylinositol 4-kinase) activities, and also the presence of PtdIns4P in cytoplasmic vesicles, which are not readily identifiable as PI4K containing trafficking intermediates. In addition, we present evidence that the majority of PtdIns4P resides in the plasma membrane, where it is metabolically distinct from the steady-state plasma membrane pool of PtdIns(4,5)P2.
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Cites
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Cited by
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Self-citationPolyglutamine tracts regulate beclin 1-dependent autophagy
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Metadata
- Authors
- Hammond, G. R. V., Schiavo, G., & Irvine, R. F.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2009-07-29
- Volume
- 422
- Issue
- 1
- Pages
- 23-35
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W2039987826
- Cited by
- 306
- References
- 54
- FWCI
- 5.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hammond, G. R. V., Schiavo, G., & Irvine, R. F. (2009). Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4 P and PtdIns(4,5) P 2. Biochemical Journal. https://doi.org/10.1042/bj20090428
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