Differential regulation by exercise of BDNF and NT-3 in rat spinal cord and skeletal muscle
Open accessAbstract
We have investigated the impact of neuromuscular activity on the expression of neurotrophins in the lumbar spinal cord region and innervating skeletal muscle of adult rats. Rats were exercised on a treadmill for 1 day or 5 consecutive days and euthanized at 0, 2 or 6 h after the last bout of exercise. By Day 1, there was no clear evidence of an increase in brain-derived neurotrophic factor (BDNF) mRNA in the spinal cord or the soleus muscle. By Day 5, there was a significant increase in BDNF mRNA in the spinal cord at 2 h post-training, and the soleus muscle showed a robust increase between 0 and 6 h post-training. Immunoassays showed significant increases in BDNF protein in the soleus muscle by training Day 5. Immunohistochemical analyses showed elevated BDNF levels in motoneuron cell bodies and axons in the ventral horn. Neurotrophin-3 (NT-3) mRNA was measured to determine whether selected neurotrophins respond with a selective pattern of induction to neuromuscular activity. In the spinal cord, there was a progressive post-training decrease in NT-3 mRNA following a single bout of training, while there was a significant increase in NT-3 mRNA at 2 h post-training by Day 5. The soleus muscle showed a progressive increase in NT-3 mRNA by Days 1 and 5 following training. These results show that neuromuscular activity has specific effects on the BDNF and NT-3 systems, and that repetitive exercise affects the magnitude and stability of these responses.
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Training increases BDNF expression in the hippocampus, cerebral cortex, ST, brainstem, and spinal cord.
“Under the influence of the training process, increased BDNF expression in the hippocampus, cerebral cortex, ST, brainstem, and spinal cord was observed [131,174,179,181,182].”
From BDNF as a Promising Therapeutic Agent in Parkinson’s Disease (2020) · cites this paper as [182]
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Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,240 · see citing works
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczyński, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 47,092 · see citing works
Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus
van Praag, H., Kempermann, G., & Gage, F. H. (1999) · Nature Neuroscience · doi:10.1038/6368 · Cited by 3,880 · see citing works
Ageing, fitness and neurocognitive function
Kramer, A. F., Hahn, S., Cohen, N. J., Banich, M. T., McAuley, E., Harrison, C. R., Chason, J., Vakil, E., Bardell, L., Boileau, R. A., et al. (1999) · Nature · doi:10.1038/22682 · Cited by 1,394 · see citing works
Neurotrophin-3: a Neurotrophic Factor Related to NGF and BDNF
Maisonpierre, P. C., Belluscio, L., Squinto, S., Ip, N. Y., Furth, M. E., Lindsay, R. M., & Yancopoulos, G. D. (1990) · Science · doi:10.1126/science.2321006 · Cited by 1,322 · see citing works
Exercise and brain neurotrophins
Neeper, S. A., Góauctemez-Pinilla, F., Choi, J., & Cotman, C. (1995) · Nature · doi:10.1038/373109a0 · Cited by 1,066 · see citing works
Neeper, S. A., Gómez‐Pinilla, F., Choi, J., & Cotman, C. W. (1996) · Brain Research · doi:10.1016/0006-8993(96)00273-9 · Cited by 1,015 · see citing works
Self-citationRecovery of locomotion after chronic spinalization in the adult cat
Barbeau, H., & Rossignol, S. (1987) · Brain Research · doi:10.1016/0006-8993(87)91442-9 · Cited by 846 · see citing works
Brain-derived neurotrophic factor rescues spinal motor neurons from axotomy-induced cell death
Qiao, Y., Elliott, J. L., & Snider, W. D. (1992) · Nature · doi:10.1038/360753a0 · Cited by 670 · see citing works
Grill, R., Murai, K. K., Blesch, A., Gage, F. H., & Tuszynski, M. H. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-14-05560.1997 · Cited by 619 · see citing works
Human Lumbosacral Spinal Cord Interprets Loading During Stepping
Harkema, S. J., Hurley, S. L., Patel, U. K., Requejo, P. S., Dobkin, B. H., & Edgerton, V. R. (1997) · Journal of Neurophysiology · doi:10.1152/jn.1997.77.2.797 · Cited by 604 · see citing works
Self-citationEvidence that brain-derived neurotrophic factor is a trophic factor for motor neurons in vivo
Koliatsos, V. E., Clatterbuck, R. E., Winslow, J. W., Cayouette, M. H., & Prices, D. L. (1993) · Neuron · doi:10.1016/0896-6273(93)90326-m · Cited by 539 · see citing works
Laufband Therapy Based on‘Rules of Spinal Locomotion’is Effective in Spinal Cord Injured Persons
Wernig, A., Müller, S., Nanassy, A., & Cagol, E. (1995) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.1995.tb00686.x · Cited by 494 · see citing works
de Leon, R. D., Hodgson, J. A., Roy, R. R., & Edgerton, V. R. (1998) · Journal of Neurophysiology · doi:10.1152/jn.1998.79.3.1329 · Cited by 471 · see citing works
Self-citationXu, X., Guénard, V., Kleitman, N., Aebischer, P., & Bunge, M. B. (1995) · Experimental Neurology · doi:10.1006/exnr.1995.1056 · Cited by 469 · see citing works
Muscle-Derived Neurotrophin-4 as an Activity-Dependent Trophic Signal for Adult Motor Neurons
Funakoshi, H., Belluardo, N., Arenas, E., Yamamoto, Y., Casabona, A., Persson, H., & Ibáñez, C. F. (1995) · Science · doi:10.1126/science.7770776 · Cited by 383 · see citing works
Gómez‐Pinilla, F., Lee, J., & Cotman, C. W. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-01-00345.1992 · Cited by 361 · see citing works
Self-citationYe, J.-H., & Houlé, J. D. (1997) · Experimental Neurology · doi:10.1006/exnr.1996.6353 · Cited by 345 · see citing works
Physical exercise induces FGF-2 and its mRNA in the hippocampus
Gómez‐Pinilla, F., Dao, L., & So, V. (1997) · Brain Research · doi:10.1016/s0006-8993(97)00375-2 · Cited by 292 · see citing works
Self-citationGómez‐Pinilla, F., So, V., & Kesslak, J. P. (1998) · Neuroscience · doi:10.1016/s0306-4522(97)00576-9 · Cited by 262 · see citing works
Self-citationEMG patterns of rat ankle extensors and flexors during treadmill locomotion and swimming
Roy, R. R., Hutchison, D. L., Pierotti, D. J., et al. (1991) · Journal of Applied Physiology · doi:10.1152/jappl.1991.70.6.2522 · Cited by 251 · see citing works
Self-citationNeurotrophins promote maturation of developing neuromuscular synapses
Wang, T., Xie, K., & Lu, B. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-07-04796.1995 · Cited by 249 · see citing works
Jakeman, L. B., Wei, P. Y., Guan, Z., & Stokes, B. T. (1998) · Experimental Neurology · doi:10.1006/exnr.1998.6924 · Cited by 214 · see citing works
BDNF and NT-4/5 exert neurotrophic influences on injured adult spinal motor neurons
Friedman, B., Kleinfeld, D., Ip, N. Y., Verge, V., Moulton, R., Boland, P., Zlotchenko, E., Lindsay, R. M., & Liu, L. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-02-01044.1995 · Cited by 208 · see citing works
Enhancement of locomotor recovery following spinal cord injury
Barbeau, H., & Rossignol, S. (1994) · Current Opinion in Neurology · doi:10.1097/00019052-199412000-00008 · Cited by 196 · see citing works
Can the mammalian lumbar spinal cord learn a motor task?
Hodgson, J. A., Roy, R. R., de Leon, R. D., Dobkin, B. H., & Edgerton, V. R. (1994) · Medicine & Science in Sports & Exercise · doi:10.1249/00005768-199412000-00013 · Cited by 194 · see citing works
Self-citationNeurobiological basis of human locomotion
Hultborn, H. (1992) · Trends in Neurosciences · doi:10.1016/0166-2236(92)90084-l · Cited by 181 · see citing works
Laufband (Treadmill) Therapy in Incomplete Paraplegia and Tetraplegia
Wernig, A., Nanassy, A., & MÜLLER, S. (1999) · Journal of Neurotrauma · doi:10.1089/neu.1999.16.719 · Cited by 160 · see citing works
Nakahara, Y., Gage, F. H., & Tuszynski, M. H. (1996) · Cell Transplantation · doi:10.1016/0963-6897(95)02028-4 · Cited by 156 · see citing works
Neurobiological basis of human locomotion
島村, 宗., Grillner, S., & Edgerton, V. R. (1991) · Japan Scientific Societies Press eBooks · Cited by 119 · see citing works
Self-citationCurtis, R., Tonra, J. R., Stark, J. L., Adryan, K. M., Park, J. S., Cliffer, K. D., Lindsay, R. M., & DiStefano, P. S. (1998) · Molecular and Cellular Neuroscience · doi:10.1006/mcne.1998.0704 · Cited by 118 · see citing works
Sagot, Y., Rossé, T., Vejsada, R., Perrelet, D., & Kato, A. C. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-03-01132.1998 · Cited by 99 · see citing works
Neeper, S. A. (1996) · Brain Research · doi:10.1016/s0006-8993(96)00273-9 · Cited by 91 · see citing works
Braun, S., Croizat, B., Lagrange, M.-C., et al. (1996) · Journal of the Neurological Sciences · doi:10.1016/0022-510x(95)00315-s · Cited by 85 · see citing works
Physical rehabilitation in neurologic diseases
Bohannon, R. W. (1993) · Current Opinion in Neurology · doi:10.1097/00019052-199310000-00015 · Cited by 73 · see citing works
Burgess, J. W., & Villablanca, J. R. (1986) · Behavioural Brain Research · doi:10.1016/0166-4328(86)90096-3 · Cited by 72 · see citing works
Mi, Z. P., Jiang, P., Weng, W., Lindberg, F. P., Narayanan, V., & Lagenaur, C. F. (2000) · The Journal of Comparative Neurology · doi:10.1002/(sici)1096-9861(20000117)416:3 · Cited by 65 · see citing works
Does Motor Learning Occur in the Spinal Cord?
Edgerton, V. R., Roy, R. R., Tillakaratne, R. D. L. N., et al. (1997) · The Neuroscientist · doi:10.1177/107385849700300510 · Cited by 65 · see citing works
Self-citationThe Benefit of a Home Exercise Regimen for Ambulatory Parkinsonʼs Disease Patients
Hurwitz, A. (1989) · Journal of Neuroscience Nursing · doi:10.1097/01376517-198906000-00008 · Cited by 60 · see citing works
Effects of BDNF and NT-3 on Development of Ia/Motoneuron Functional Connectivity in Neonatal Rats
Seebach, B. S., Arvanov, V. L., & Mendell, L. M. (1999) · Journal of Neurophysiology · doi:10.1152/jn.1999.81.5.2398 · Cited by 60 · see citing works
Cerebral Protection Against Ischemia by Locomotor Activity in Gerbils
Stummer, W., Baethmann, A. J., Murr, R., Schürer, L., & Kempski, O. S. (1995) · Stroke · doi:10.1161/01.str.26.8.1423 · Cited by 53 · see citing works
Exercise and sport in mental health: a review of the literature.
Gleser, J. M., & Mendelberg, H. E. (1990) · PubMed · Cited by 49 · see citing works
Rehabilitation of the neurologically disabled patient: principles, practice, and scientific basis
Perry, J. (1983) · Journal of neurosurgery · doi:10.3171/jns.1983.58.6.0799 · Cited by 16 · see citing works
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Fiuza-Luces, C., Garatachea, N., Berger, N. A., & Lucia, A. (2013) · Physiology · doi:10.1152/physiol.00019.2013 · Cited by 791 · see citing works
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- Authors
- Gómez‐Pinilla, F., Ying, Z., Opazo, P., Roy, R. R., & Edgerton, V. R.
- Affiliations
- University of California, Los Angeles (US)
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2001-03
- Volume
- 13
- Issue
- 6
- Pages
- 1078-1084
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2068181807
- Cited by
- 362
- References
- 43
- Citations by year
- 2026: 2 · 2025: 9 · 2024: 3 · 2023: 12 · 2022: 16 · 2021: 10 · 2020: 13 · 2019: 11 · 2018: 20 · 2017: 11 · 2016: 20 · 2015: 19 · 2014: 24 · 2013: 16 · 2012: 20
- FWCI
- 5.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ns038978-03)
- National Institute of Neurological Disorders and Stroke (NS 39522, NS-38978, R01NS039522)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- physical activity, brain-derived neurotrophic factor, neurotrophin-3, neuromuscular activity, lumbar spinal cord, soleus muscle, ventral horn motoneurons, neurotrophin gene expression, treadmill training, repeated exercise
- MeSH terms
- Animals, Motor Activity, RNA, Messenger, Spinal Cord, Rats, Sprague-Dawley, Muscle, Skeletal, Brain-Derived Neurotrophic Factor, Neurotrophin 3, Rats
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