Equid herpesvirus 1 infection of endothelial cells requires activation of putative adhesion molecules: an in vitro model
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Antisera to activated equine endothelial cells, which detected surface molecules of 116 kD, 97 kD, 42 kD and 38 kD, were made to investigate the role of endothelial adhesion molecules in equid herpes virus 1 infection. These putative adhesion molecules could be induced by 17-beta oestradiol, chorionic gonadotrophin, or IL-2, as well as by LPS and PWM. In an in vitro flow system, using equine veins or arteries, equid herpesvirus 1 in leucocytes was only transferred to infect endothelial cells if both leucocytes and endothelial cells expressed these surface molecules. Blocking of the membrane molecules with polyclonal antibodies prevented transfer of virus to the endothelial cells, indicating that the adhesion molecules had a key role in effecting transfer of virus. These in vitro observations give particular insight into the reports that in the natural course of infection in horses infection of endothelial cells is restricted to certain tissues, and in a wider context the results illustrate the complexity of factors that may direct tissue tropism.
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Cytokines (e.g., IL-2) and hormones (e.g., 17-estradiol and equine chorionic gonadotropin) upregulate expression of adhesion molecules on endothelial cells.
“It was demonstrated that cytokines (e.g., IL-2) and hormones (e.g., 17-estradiol and equine chrorionic gonadotropin) upregulate the expression of adhesion molecules on EC (Smith et al., 2002).”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Smith et al., 2002
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- Authors
- SMITH, D., HAMBLIN, A., & EDINGTON, N.
- Affiliations
- Royal Veterinary College (GB)
- Venue
- Clinical and Experimental Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-08
- Volume
- 129
- Issue
- 2
- Pages
- 281-287
- ISSN
- 0009-9104 (ISSN-L)
- 1365-2249
- PMC
- PMC1906444
- OpenAlex
- W2023120099
- Cited by
- 30
- References
- 29
- Citations by year
- 2024: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 2
- FWCI
- 1.26
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Clinical & Experimental Immunology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- RVC Research Online (Royal Veterinary College) (submittedVersion, cc-by)
- Funders
- Horserace Betting Levy Board
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Keywords
- equine herpesvirus 1, endothelial cells, adhesion molecules, endothelial activation, tissue tropism, leukocytes, 17 beta-oestradiol, human chorionic gonadotropin, interleukin-2, lipopolysaccharide, polyclonal antibodies, horses
- MeSH terms
- Animals, Antibodies, Cell Adhesion, Endothelium, Vascular, Herpesvirus 1, Equid, Female, Herpesviridae Infections, Horse Diseases, Horses, Leukocytes, Cell Adhesion Molecules, In Vitro Techniques
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