Cited by 30Herpesvirus Infections and TreatmentsCell Adhesion Molecules ResearchAnimal Virus Infections Studies

Equid herpesvirus 1 infection of endothelial cells requires activation of putative adhesion molecules: an in vitro model

Open access
SMITH, D, HAMBLIN, A, EDINGTON, N·2002·Clinical and Experimental Immunology

Abstract

Antisera to activated equine endothelial cells, which detected surface molecules of 116 kD, 97 kD, 42 kD and 38 kD, were made to investigate the role of endothelial adhesion molecules in equid herpes virus 1 infection. These putative adhesion molecules could be induced by 17-beta oestradiol, chorionic gonadotrophin, or IL-2, as well as by LPS and PWM. In an in vitro flow system, using equine veins or arteries, equid herpesvirus 1 in leucocytes was only transferred to infect endothelial cells if both leucocytes and endothelial cells expressed these surface molecules. Blocking of the membrane molecules with polyclonal antibodies prevented transfer of virus to the endothelial cells, indicating that the adhesion molecules had a key role in effecting transfer of virus. These in vitro observations give particular insight into the reports that in the natural course of infection in horses infection of endothelial cells is restricted to certain tissues, and in a wider context the results illustrate the complexity of factors that may direct tissue tropism.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1046/j.1365-2249.2002.01463.x

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Cytokines (e.g., IL-2) and hormones (e.g., 17-estradiol and equine chorionic gonadotropin) upregulate expression of adhesion molecules on endothelial cells.

    “It was demonstrated that cytokines (e.g., IL-2) and hormones (e.g., 17-estradiol and equine chrorionic gonadotropin) upregulate the expression of adhesion molecules on EC (Smith et al., 2002).”

    From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Smith et al., 2002

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 5

  1. Phinney PR, Fligiel S, BrysonYJ, Porter DD. Necrotising vasculitis in a case of disseminated neonatal herpesvirus infection. Arch Path Lab Med 1982; 106:64-7.
  2. Bridges CG, Edington N. Genetic restriction of cytotoxic T cell activ- ity against subtype 2 of equid herpes virus 1 in ponies. Clin & exp immunol. 1987; 70:276-282.
  3. Charan S, Palmer K, Meager AA, Meyer-Sluis A, Edington N. TGFB induced by live or UV inactivated Equid herpesvirus-1 [EHV-1] mediates immunosuppression in the horse. Immnunol 1997; 90:586-91.
  4. Smith D. The interactions of Equid herpes virus-1 infected leucocytes with equine endothelial cells. PhD Thesis. Department of Pathology and Infections Disease, Royal Veterinary College, University of London. 2000.
  5. Pober JS, Lapierre LA, Stolpen AH et al. Activation of cultured endothelial cells by recombinant lymphotoxin: a comparison with TNF and IL species. J Immunol 1987; 138:3319-24.

Cited by

Sorted by citations

Authors
SMITH, D., HAMBLIN, A., & EDINGTON, N.
Affiliations
Royal Veterinary College (GB)
Venue
Clinical and Experimental Immunology
Published
2002-08
Volume
129
Issue
2
Pages
281-287
ISSN
  • 0009-9104 (ISSN-L)
  • 1365-2249
OpenAlex
W2023120099
Cited by
30
References
29
Citations by year
2024: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 2
FWCI
1.26
Type
journal article
Language
en
Open access
Locations
  • Clinical & Experimental Immunology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • RVC Research Online (Royal Veterinary College) (submittedVersion, cc-by)
Funders
Horserace Betting Levy Board
Topics
  • Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
Keywords
equine herpesvirus 1, endothelial cells, adhesion molecules, endothelial activation, tissue tropism, leukocytes, 17 beta-oestradiol, human chorionic gonadotropin, interleukin-2, lipopolysaccharide, polyclonal antibodies, horses
MeSH terms
Animals, Antibodies, Cell Adhesion, Endothelium, Vascular, Herpesvirus 1, Equid, Female, Herpesviridae Infections, Horse Diseases, Horses, Leukocytes, Cell Adhesion Molecules, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review