The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Two domains of Nrf2 cooperatively bind CBP, a CREB binding protein, and synergistically activate transcription
Open accessAbstract
Background Nrf2 belongs to the Cap‐N‐Collar (CNC) transcription factor family and is essential for the antioxidant responsive element (ARE)‐mediated expression of a group of detoxifying and antioxidant genes. The forced expression of Nrf2 in mammalian cells activates the expression of target genes through the ARE, with Nrf2 showing the highest transactivation activity among the CNC family of transcription factors. To elucidate the molecular mechanisms generating this potent transactivation activity, we examined the functions of the domains within Nrf2. Result We found that Nrf2 contains two transcription activation domains, Neh4 and Neh5, which act synergistically to attain maximum a activation of reporter gene expression. Neh4 and Neh5 both individually and cooperatively bind to CBP (C REB (c AMP R esponsive E lement B inding protein) B inding P rotein). In fact, the specific inhibitor of CBP, adenovirus E1A protein, significantly reduced Nrf2 activity. Importantly, the CBP‐binding activity of Nrf2 deletion mutants positively correlated with their transactivation activity. Neh5 contains a motif which is commonly conserved among the CNC factors, whereas Neh4 contains the novel CBP‐interacting motif recently identified in p53 and E2F. Conclusions Our results indicate that Nrf2 exploits the cooperative binding of two independent transactivation domains to CBP in the acquisition of a potent transactivation activity.
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Itoh, K., Wakabayashi, N., Katoh, Y., Ishii, T., Igarashi, K., Engel, J. D., & Yamamoto, M. (1999) · Genes & Development · doi:10.1101/gad.13.1.76 · Cited by 3,634
Self-citationCBP/p300 in cell growth, transformation, and development
Goodman, R. H., & Smolik, S. (2000) · Genes & Development · doi:10.1101/gad.14.13.1553 · Cited by 1,778
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Transcriptional activation by recruitment
Ptashne, M., & Gann, A. (1997) · Nature · doi:10.1038/386569a0 · Cited by 1,158 · cited 1×
“The coactivator recruitment step is one of the ratelimiting steps in gene transcription (Dorris & Struhl 2000; Ptashne & Gann 1997).”
BackgroundZhang, W., & Bieker, J. J. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.17.9855 · Cited by 342
Kim, T. K., Kim, T. H., & Maniatis, T. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.21.12191 · Cited by 109
Functional Association between CBP and HNF4 inTrans-activation
Yoshida, E., Aratani, S., Itou, H., Miyagishi, M., Takiguchi, M., Osumu, T., Murakami, K., & Fukamizu, A. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.7871 · Cited by 104
Self-citationCheng, X., Reginato, M. J., Andrews, N. C., & Lazar, M. A. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.3.1407 · Cited by 102
Cited by
The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress
Nguyen, T., Nioi, P., & Pickett, C. B. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.r900010200 · Cited by 2,655
The KEAP1-NRF2 system: a thiol-based sensor-effector apparatus for maintaining redox homeostasis
Yamamoto, M., Kensler, T. W., & Motohashi, H. (2018) · Physiological Reviews · doi:10.1152/physrev.00023.2017 · Cited by 1,968
Self-citationNrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription
Kobayashi, E. H., Suzuki, T., Funayama, R., Nagashima, T., Hayashi, M., Sekine, H., Tanaka, N., Moriguchi, T., Motohashi, H., Nakayama, K., et al. (2016) · Nature Communications · doi:10.1038/ncomms11624 · Cited by 1,878
Self-citationNRF2, a Transcription Factor for Stress Response and Beyond
He, F., Ru, X., & Wen, T. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21134777 · Cited by 1,820
Nrf2 signaling pathway: pivotal roles in inflammation
Ahmed, S. M. U., Luo, L., Namani, A., Wang, X. J., & Tang, X. (2017) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2016.11.005 · Cited by 1,794
Metadata
- Authors
- Katoh, Y., Itoh, K., Yoshida, E., Miyagishi, M., Fukamizu, A., & Yamamoto, M.
- Venue
- Genes to Cells
- Publisher
- Wiley
- Published
- 2001-10
- Volume
- 6
- Issue
- 10
- Pages
- 857-868
- ISSN
- 1356-9597 (ISSN-L)
- 1365-2443
- OpenAlex
- W1990598806
- Cited by
- 520
- References
- 9
- FWCI
- 3.37
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Katoh, Y., Itoh, K., Yoshida, E., Miyagishi, M., Fukamizu, A., & Yamamoto, M. (2001). Two domains of Nrf2 cooperatively bind CBP, a CREB binding protein, and synergistically activate transcription. Genes to Cells. https://doi.org/10.1046/j.1365-2443.2001.00469.x
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