The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
I5M: 3D widefield light microscopy with better than 100 nm axial resolution
Abstract
Sevenfold improved axial resolution has been achieved in three‐dimensional widefield fluorescence microscopy, using a novel interferometric technique in which the sample is observed and/or illuminated from both sides simultaneously using two opposing objective lenses. Separate interference effects in the excitation light and the emitted light give access to higher resolution axial information about the sample than can be reached by conventional widefield or confocal microscopes. Here we report the experimental verification of this resolution performance on complex biological samples.
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Citations
Cites
Handbook Of Biological Confocal Microscopy
(2006) · Springer eBooks · doi:10.1007/978-0-387-45524-2 · Cited by 3,637
Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung
Abbe, E. (1873) · Archiv für Mikroskopische Anatomie · doi:10.1007/bf02956173 · Cited by 2,724
Chapter 13 Fluorescence Microscopy in Three Dimensions
Agard, D. A., Hiraoka, Y., Shaw, P., & Sedat, J. W. (1989) · Methods in Cell Biology · doi:10.1016/s0091-679x(08)60986-3 · Cited by 649
Properties of a 4Pi confocal fluorescence microscope
Hell, S., & Stelzer, E. H. K. (1992) · Journal of the Optical Society of America A · doi:10.1364/josaa.9.002159 · Cited by 560
Handbook of Biological Confocal Microscopy
(1990) · Springer eBooks · doi:10.1007/978-1-4615-7133-9 · Cited by 433
Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation
Bailey, B., Farkas, D. L., Taylor, D. L., & Lanni, F. (1993) · Nature · doi:10.1038/366044a0 · Cited by 324
Sevenfold improvement of axial resolution in 3D wide-field microscopy using two objective lenses
Gustafsson, M. G. L., Agard, D. A., & Sedat, J. W. (1995) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.205334 · Cited by 157
Far‐field fluorescence microscopy with three‐dimensional resolution in the 100‐nm range
Hell, S. W., Schrader, M., & van der Voort, H. T. M. (1997) · Journal of Microscopy · doi:10.1046/j.1365-2818.1997.2410797.x · Cited by 127
Three-dimensional super-resolution with a 4Pi-confocal microscope using image restoration
Schrader, M., Hell, S. W., & van der Voort, H. T. M. (1998) · Journal of Applied Physics · doi:10.1063/1.368616 · Cited by 92
Cited by
Gustafsson, M. G. L. (2000) · Journal of Microscopy · doi:10.1046/j.1365-2818.2000.00710.x · Cited by 3,786
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
The third dimension bridges the gap between cell culture and live tissue
Pampaloni, F., Reynaud, E. G., & Stelzer, E. H. K. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2236 · Cited by 2,817
Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission
Klar, T. A., Jakobs, S., Dyba, M., Egner, A., & Hell, S. W. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.15.8206 · Cited by 1,869
Super-resolution fluorescence microscopy
Huang, B., Bates, M., & Zhuang, X. (2009) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.77.061906.092014 · Cited by 1,657
Metadata
- Authors
- Gustafsson, Agard, & Sedat
- Venue
- Journal of Microscopy
- Publisher
- Wiley
- Published
- 1999-07
- Volume
- 195
- Issue
- 1
- Pages
- 10-16
- ISSN
- 0022-2720 (ISSN-L)
- 1365-2818
- OpenAlex
- W2089726895
- Cited by
- 417
- References
- 9
- FWCI
- 7.78
- Type
- journal article
- Language
- en
- Open access
- closed
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- Gustafsson, Agard, & Sedat. (1999). I5M: 3D widefield light microscopy with better than 100 nm axial resolution. Journal of Microscopy. https://doi.org/10.1046/j.1365-2818.1999.00576.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
