The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Empirical evaluation of preservation methods for faecal DNA
Abstract
We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free‐ranging baboons showed that amplification success was dependent on preservation method, PCR‐product size, and whether nuclear or mitochondrial DNA was assayed. Storage in a DMSO/EDTA/Tris/salt solution (DETs) was most effective for preserving nuclear DNA, but storage in 70% ethanol, freezing at –20°C and drying performed approximately equally well for mitochondrial DNA and short (<200 bp) nuclear DNA fragments. Because faecal DNA is diluted and degraded, repeated extractions from faeces may be necessary and short nuclear markers should be employed for genotyping. A review of molecular scatology studies further suggests that three to six faeces per individual should be collected.
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Cites
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Noninvasive genetic sampling: look before you leap
Taberlet, P., Waits, L. P., & Luikart, G. (1999) · Trends in Ecology & Evolution · doi:10.1016/s0169-5347(99)01637-7 · Cited by 1,078
Human Gut Microbiome and Risk for Colorectal Cancer
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WAITS, L. P., & PAETKAU, D. (2005) · Journal of Wildlife Management · doi:10.2193/0022-541x(2005)69[1419:ngstfw]2.0.co;2 · Cited by 720
INVITED REVIEW: Molecular analysis of predation: a review of best practice for DNA‐based approaches
KING, R. A., READ, D. S., TRAUGOTT, M., & SYMONDSON, W. O. C. (2008) · Molecular Ecology · doi:10.1111/j.1365-294x.2007.03613.x · Cited by 657
Noninvasive molecular tracking of colonizing wolf (Canis lupus) packs in the western Italian Alps
Lucchini, V., Fabbri, E., Marucco, F., Ricci, S., Boitani, L., & Randi, E. (2002) · Molecular Ecology · doi:10.1046/j.1365-294x.2002.01489.x · Cited by 301
Metadata
- Authors
- FRANTZEN, M. A. J., SILK, J. B., FERGUSON, J. W. H., WAYNE, R. K., & KOHN, M. H.
- Venue
- Molecular Ecology
- Publisher
- Wiley
- Published
- 1998-10
- Volume
- 7
- Issue
- 10
- Pages
- 1423-1428
- ISSN
- 0962-1083 (ISSN-L)
- 1365-294X
- OpenAlex
- W2166933228
- Cited by
- 285
- References
- 26
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- closed
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- FRANTZEN, M. A. J., SILK, J. B., FERGUSON, J. W. H., WAYNE, R. K., & KOHN, M. H. (1998). Empirical evaluation of preservation methods for faecal DNA. Molecular Ecology. https://doi.org/10.1046/j.1365-294x.1998.00449.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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