Aromatic components of two ferric enterobactin binding sites in Escherichia coli FepA
Open accessAbstract
Ferric enterobactin is a catecholate siderophore that binds with high affinity (Kd ≈ 10−10 M) to the Escherichia coli outer membrane protein FepA. We studied the involvement of aromatic amino acids in its uptake by determining the binding affinities, kinetics and transport properties of site‐directed mutants. We replaced seven aromatic residues (Y260, Y272, Y285, Y289, W297, Y309 and F329) in the central part of FepA primary structure with alanine, individually and in double combinations, and determined the ability of the mutant proteins to interact with ferric enterobactin and the protein toxins colicins B and D. All the constructs showed normal expression and localization. Among single mutants, Y260A and F329A were most detrimental, reducing the affinity between FepA and ferric enterobactin 100‐ and 10‐fold respectively. Double substitutions involving Y260, Y272 and F329 impaired (100‐ to 2500‐fold) adsorption of the iron chelate more strongly. For Y260A and Y272A, the drop in adsorption affinity caused commensurate decreases in transport efficiency, suggesting that the target residues primarily act in ligand binding. F329A, like R316A, showed greater impairment of transport than binding, intimating mechanistic involvement during ligand internalization. Furthermore, immunochemical studies localized F329 in the FepA ligand binding site. The mutagenesis results suggested the existence of dual ligand binding sites in the FepA vestibule, and measurements of the rate of ferric enterobactin adsorption to fluoresceinated FepA mutant proteins confirmed this conclusion. The initial, outermost site contains aromatic residues and probably functions through hydrophobic interactions, whereas the secondary site exists deeper in the vestibule, contains both charged and aromatic residues and probably acts through hydrophobic and electrostatic bonds.
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Larger halos generally correlate with slower FeEnt uptake.
“Larger halos generally correlate with slower FeEnt uptake (32,33).”
From Siderophore-mediated iron acquisition by <i>Klebsiella pneumoniae</i> (2024) · cites this paper as [33]
The Transport section (page unavailable) reports larger halos among FepA mutants with transport deficiencies, which supports an association between halo size and impaired FeEnt uptake. It does not establish a general correlation with slower uptake rates: the same section says that only R316A reproducibly reduced the maximum transport rate, and only modestly. (LLM, conf=0.83)
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Cites
Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157 · see citing works
Rapid and efficient site-specific mutagenesis without phenotypic selection.
Kunkel, T. A. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.2.488 · Cited by 7,309 · see citing works
Culture Medium for Enterobacteria
Neidhardt, F. C., Bloch, P. L., & Smith, D. F. (1974) · Journal of Bacteriology · doi:10.1128/jb.119.3.736-747.1974 · Cited by 2,977 · see citing works
Principles of protein-protein interactions
Jones, S., & Thornton, J. M. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.1.13 · Cited by 2,684 · see citing works
The maximal affinity of ligands
Kuntz, I. D., Chen, K., Sharp, K. A., & Kollman, P. A. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.18.9997 · Cited by 859 · see citing works
Siderophore-Mediated Iron Transport: Crystal Structure of FhuA with Bound Lipopolysaccharide
Ferguson, A. D., Hofmann, E., Coulton, J. W., Diederichs, K., & Welte, W. (1998) · Science · doi:10.1126/science.282.5397.2215 · Cited by 787 · see citing works
Aromatic rings act as hydrogen bond acceptors
Levitt, M., & Perutz, M. F. (1988) · Journal of Molecular Biology · doi:10.1016/0022-2836(88)90471-8 · Cited by 697 · see citing works
Crystal structure of the outer membrane active transporter FepA from Escherichia coli.
Buchanan (1999) · Nature Structural Biology · doi:10.1038/4931 · Cited by 591 · see citing works
Transmembrane Signaling across the Ligand-Gated FhuA Receptor
Locher, K. P., Rees, B., Koebnik, R., Mitschler, A., Moulinier, L., Rosenbusch, J. P., & Moras, D. (1998) · Cell · doi:10.1016/s0092-8674(00)81700-6 · Cited by 540 · see citing works
Amino/Aromatic Interactions in Proteins: Is the Evidence Stacked Against Hydrogen Bonding?
Mitchell, J. B. O., Nandi, C. L., McDonald, I. K., Thornton, J. M., & Price, S. L. (1994) · Journal of Molecular Biology · doi:10.1006/jmbi.1994.1370 · Cited by 303 · see citing works
Planar Stacking Interactions of Arginine and Aromatic Side-Chains in Proteins
Flocco, M. M., & Mowbray, S. L. (1994) · Journal of Molecular Biology · doi:10.1006/jmbi.1994.1022 · Cited by 295 · see citing works
Molecular dynamics and free-energy calculations applied to affinity maturation in antibody 48G7
Chong, L. T., Duan, Y., Wang, L., Massova, I., & Kollman, P. A. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.25.14330 · Cited by 250 · see citing works
Lundrigan, M. D., & Kadner, R. J. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)67457-5 · Cited by 209 · see citing works
Formation of a Gated Channel by a Ligand-Specific Transport Protein in the Bacterial Outer Membrane
Rutz, J. M., Liu, J., Lyons, J. A., Goranson, J., Armstrong, S. K., McIntosh, M. A., Feix, J. B., & Klebba, P. E. (1992) · Science · doi:10.1126/science.1411544 · Cited by 165 · see citing works
Self-citationIroN, a Novel Outer Membrane Siderophore Receptor Characteristic of <i>Salmonella enterica</i>
Bäumler, A. J., Norris, T. L., Lasco, T., Voigt, W., Reissbrodt, R., Rabsch, W., & Heffron, F. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.6.1446-1453.1998 · Cited by 147 · see citing works
Killmann, H., Benz, R., & Braun, V. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb05969.x · Cited by 134 · see citing works
Surface topology of the Escherichia coli K-12 ferric enterobactin receptor
Murphy, C. K., Kalve, V. I., & Klebba, P. E. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.5.2736-2746.1990 · Cited by 115 · see citing works
Self-citationDean, C. R., & Poole, K. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.2.317-324.1993 · Cited by 112 · see citing works
Kinetics of Biosynthesis of Iron-Regulated Membrane Proteins in Escherichia coli
Klebba, P. E., McIntosh, M. A., & Neilands, J. B. (1982) · Journal of Bacteriology · doi:10.1128/jb.149.3.880-888.1982 · Cited by 108 · see citing works
Self-citationLigand-Specific Opening of a Gated-Porin Channel in the Outer Membrane of Living Bacteria
Jiang, X., Payne, M. A., Cao, Z., Foster, S. B., Feix, J. B., Newton, S. M. C., & Klebba, P. E. (1997) · Science · doi:10.1126/science.276.5316.1261 · Cited by 92 · see citing works
Self-citationEffect of loop deletions on the binding and transport of ferric enterobactin by FepA
Newton, S. M. C., Igo, J. D., Scott, D. C., & Klebba, P. E. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01424.x · Cited by 91 · see citing works
Self-citationDavis, S. J., Davies, E., Tucknott, M. G., Jones, E. Y., & van der Merwe, P. A. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.10.5490 · Cited by 81 · see citing works
Selectivity of Ferric Enterobactin Binding and Cooperativity of Transport in Gram-Negative Bacteria
Thulasiraman, P., Newton, S. M. C., Xu, J., Raymond, K. N., Mai, C., Hall, A., Montague, M. A., & Klebba, P. E. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.24.6689-6696.1998 · Cited by 74 · see citing works
Self-citationPermeability properties of a large gated channel within the ferric enterobactin receptor, FepA.
Liu, J., Rutz, J. M., Feix, J. B., & Klebba, P. E. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.22.10653 · Cited by 71 · see citing works
Self-citationBiphasic Binding Kinetics between FepA and Its Ligands
Payne, M. A., Igo, J. D., Cao, Z., Foster, S. B., Newton, S. M. C., & Klebba, P. E. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.35.21950 · Cited by 59 · see citing works
Self-citationA Bordetella pertussis fepA homologue required for utilization of exogenous ferric enterobactin
Beall, B., & Sanden, G. N. (1995) · Microbiology · doi:10.1099/13500872-141-12-3193 · Cited by 59 · see citing works
Newton, S. M. C., Allen, J. S., Cao, Z., Qi, Z., Jiang, X., Sprencel, C., Igo, J. D., Foster, S. B., Payne, M. A., & Klebba, P. E. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.9.4560 · Cited by 53 · see citing works
Self-citationMolecular analysis of the Escherichia coli ferric enterobactin receptor FepA.
Armstrong, S. K., Francis, C. L., & McIntosh, M. A. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)77336-5 · Cited by 44 · see citing works
American Society for Microbiology: Annual Meeting
Eisenstein, T. K. (1992) · Defense Technical Information Center (DTIC) · Cited by 32 · see citing works
Kuhn, S., Nardin, A., Klebba, P. E., & Taylor, R. P. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.160.10.5088 · Cited by 28 · see citing works
Self-citation
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1 of the 31 references couldn’t be matched to a known paper.
Research works printed but not linked: 1
- [5]5. Cao, Z. (1999) PhD Thesis. University of Oklahoma.
Cited by
Sorted by citations
Goetz, D. H., Holmes, M. A., Borregaard, N., Bluhm, M. E., Raymond, K. N., & Strong, R. K. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00708-6 · Cited by 1,355 · see citing works
Enterobactin: an archetype for microbial iron transport.
Raymond, K. N., Dertz, E. A., & Kim, S. S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0630018100 · Cited by 933 · see citing works
Klebba, P. E., Newton, S. M. C., Six, D. A., Kumar, A., Yang, T., Nairn, B. L., Munger, C., & Chakravorty, S. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01005 · Cited by 150 · see citing works
Self-citationLüscher, A., Moynié, L., Auguste, P. S., Bumann, D., Mazza, L., Pletzer, D., Naismith, J. H., & Köhler, T. (2018) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00097-18 · Cited by 126 · see citing works
Moynié, L., Lüscher, A., Rolo, D., Pletzer, D., Tortajada, A., Weingart, H., Braun, Y., Page, M. G. P., Naismith, J. H., & Köhler, T. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02531-16 · Cited by 122 · see citing works
Schalk, I. J. (2008) · Journal of Inorganic Biochemistry · doi:10.1016/j.jinorgbio.2007.11.017 · Cited by 119 · see citing works
Moynié, L., Milenkovic, S., Mislin, G. L. A., Gasser, V., Malloci, G., Baco, E., McCaughan, R. P., Page, M. G. P., Schalk, I. J., Ceccarelli, M., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-11508-y · Cited by 110 · see citing works
FepA- and TonB-Dependent Bacteriophage H8: Receptor Binding and Genomic Sequence
Rabsch, W., Ma, L. M., Wiley, G. B., Najar, F. Z., Kaserer, W. A., Schürch, D., Klebba, J. E., Roe, B. A., Gomez, J. A. L., Schallmey, M., et al. (2007) · Journal of Bacteriology · doi:10.1128/jb.00437-07 · Cited by 95 · see citing works
Self-citationMechanisms of colicin binding and transport through outer membrane porins
Cao, Z., & Klebba, P. E. (2002) · Biochimie · doi:10.1016/s0300-9084(02)01455-4 · Cited by 89 · see citing works
Self-citationEvidence of Ball-and-chain Transport of Ferric Enterobactin through FepA
Ma, L., Kaserer, W., Annamalai, R., Scott, D. C., Jin, B., Jiang, X., Xiao, Q., Maymani, H., Massis, L. M., Ferreira, L. C. S., et al. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m605333200 · Cited by 84 · see citing works
Self-citationRecognition of Ferric Catecholates by FepA
Annamalai, R., Jin, B., Cao, Z., Newton, S. M. C., & Klebba, P. E. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.11.3578-3589.2004 · Cited by 73 · see citing works
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Scott, D. C., Cao, Z., Qi, Z., Bauler, M. N., Igo, J. D., Newton, S. M. C., & Klebba, P. E. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m011282200 · Cited by 64 · see citing works
Self-citationZeng, X., Xu, F., & Lin, J. (2009) · Infection and Immunity · doi:10.1128/iai.00666-09 · Cited by 55 · see citing works
Three paradoxes of ferric enterobactin uptake
Klebba, P. E. (2003) · Frontiers in bioscience · doi:10.2741/1233 · Cited by 56 · see citing works
Self-citationQuatrini, R., Jedlicki, E., & Holmes, D. S. (2005) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-005-0233-2 · Cited by 53 · see citing works
Insight from TonB Hybrid Proteins into the Mechanism of Iron Transport through the Outer Membrane
Kaserer, W. A., Jiang, X., Xiao, Q., Scott, D. C., Bauler, M. N., Copeland, D., Newton, S. M. C., & Klebba, P. E. (2008) · Journal of Bacteriology · doi:10.1128/jb.00135-08 · Cited by 51 · see citing works
Self-citationBarnard, T. J., Watson, M. E., & McIntosh, M. A. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02473.x · Cited by 51 · see citing works
Siderophore-mediated iron acquisition by <i>Klebsiella pneumoniae</i>
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Self-citationSpectroscopic Observations of Ferric Enterobactin Transport
Cao, Z., Warfel, P., Newton, S. M. C., & Klebba, P. E. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m210360200 · Cited by 39 · see citing works
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Newton, S. M., Trinh, V., Pi, H., & Klebba, P. E. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.100206 · Cited by 38 · see citing works
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Hollander, A. M., Mercante, A. D., Shafer, W. M., & Cornelissen, C. N. (2011) · Infection and Immunity · doi:10.1128/iai.05806-11 · Cited by 38 · see citing works
Scott, D. C., Newton, S. M. C., & Klebba, P. E. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.17.4906-4911.2002 · Cited by 35 · see citing works
Self-citationde Zamaroczy, M., & Buckingham, R. H. (2002) · Biochimie · doi:10.1016/s0300-9084(02)01426-8 · Cited by 35 · see citing works
Motz, R. N., Guo, C., Sargun, A., Walker, G. T., Sassone‐Corsi, M., Raffatellu, M., & Nolan, E. M. (2024) · Journal of the American Chemical Society · doi:10.1021/jacs.3c14490 · Cited by 35 · see citing works
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Self-citationSauter, A., & Braun, V. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.16.5303-5310.2004 · Cited by 31 · see citing works
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Nairn, B. L., Eliasson, O. S., Hyder, D. R., Long, N. J., Majumdar, A., Chakravorty, S., McDonald, P. R., Roy, A., Newton, S. M. C., & Klebba, P. E. (2017) · Journal of Bacteriology · doi:10.1128/jb.00889-16 · Cited by 30 · see citing works
Self-citationZheng, Y., Kong, S., Luo, S., Chen, C., Cui, Z., Sun, X. H., Chen, T., & Wang, Z. (2022) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.1c05783 · Cited by 28 · see citing works
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Self-citationEkundayo, T. C., & Okoh, A. I. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.03077 · Cited by 24 · see citing works
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Self-citationCarswell, C. L., Rigden, M. D., & Baenziger, J. E. (2008) · Journal of Bacteriology · doi:10.1128/jb.00298-08 · Cited by 20 · see citing works
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Smallwood, C., Jordan, L. D., Trinh, V., Schürch, D., Gala, A., Hanson, M., Shipelskiy, Y., Majumdar, A., Newton, S. M. C., & Klebba, P. E. (2014) · The Journal of General Physiology · doi:10.1085/jgp.20131115907012014c · Cited by 19 · see citing works
Self-citationLópez, C. S., Alice, A. F., Chakraborty, R., & Crosa, J. H. (2007) · Microbiology · doi:10.1099/mic.0.2006/001735-0 · Cited by 18 · see citing works
Majumdar, A., Trinh, V., Moore, K. J., Smallwood, C. R., Kumar, A., Yang, T., Scott, D. C., Long, N. J., Newton, S. M., & Klebba, P. E. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.ra119.011850 · Cited by 12 · see citing works
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Luo, V. C., & Peczuh, M. W. (2024) · Molecules · doi:10.3390/molecules29163889 · Cited by 11 · see citing works
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Schalk, I. J. (2006) · Springer eBooks · doi:10.1007/0-387-28881-3_1 · Cited by 6 · see citing works
Kim, S., Park, H., Kim, D. Y., Joh, H., Oh, J. Y., Kim, D. H., Kang, M. J., Choi, C. H., & Kim, H. J. (2025) · RSC Chemical Biology · doi:10.1039/d4cb00293h · Cited by 4 · see citing works
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Crumbliss, A. L., & Siburt, C. J. P. (2012) · Wiley eBooks · doi:10.1002/9781118165850.ch13 · Cited by 2 · see citing works
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Hollander, A. M. (2011) · VCU Scholars Compass (Virginia Commonwealth University) · doi:10.25772/5jsv-wt28
influence of hydrodynamic conditions on bacteria physiology and behavior
Gonçalves, L. V. (2013) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) · doi:10.34626/ybc3-hd26
Xu, J., Fan, Y., Qu, G., Li, J., Peng, Y., Wang, M., Zhang, J., Feng, Y., Liu, X., Hu, Y., et al. (2026) · mSystems · doi:10.1128/msystems.00198-26
- Authors
- Cao, Z., Qi, Z., Sprencel, C., Newton, S. M. C., & Klebba, P. E.
- Affiliations
- University of Oklahoma (US)
- Corresponding author
- Klebba, Phillip E.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2000-09
- Volume
- 37
- Issue
- 6
- Pages
- 1306-1317
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2105974778
- Cited by
- 45
- References
- 31
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 1.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (9709418)
- Oklahoma Center for the Advancement of Science and Technology
- National Institutes of Health
- National Institute of General Medical Sciences (GM 53836, R01GM053836)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- FepA, siderophore uptake, aromatic amino acids, ligand binding sites, catecholate siderophores, outer membrane proteins, site-directed mutagenesis, hydrophobic interactions, electrostatic interactions, Escherichia coli
- MeSH terms
- Alanine, Amino Acid Sequence, Amino Acids, Cyclic, Bacterial Outer Membrane Proteins, Binding Sites, Carrier Proteins, Colicins, Enterobactin, Escherichia coli, Iron, Kinetics, Molecular Sequence Data, Receptors, Cell Surface, Mutagenesis, Site-Directed, Sequence Homology, Amino Acid, Amino Acid Substitution, Protein Transport
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